Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 236,569 C→T A168V (GCC→GTC)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5690CT98.6% 240.8 / ‑5.2 72A168V (GCC→GTC) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (28/43);  total (28/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAA  >  NC_000913/236472‑236663
                                                                                                 |                                                                                              
aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGtcc                                                                                              <  2:363949/100‑1 (MQ=255)
aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGtcc                                                                                              <  2:363921/100‑1 (MQ=255)
 aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCa                                                                                             <  1:340838/100‑1 (MQ=255)
   aTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGa                                                                                           <  1:448888/100‑1 (MQ=255)
    tGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGa                                                                                           <  1:1404701/99‑1 (MQ=255)
    tGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGAt                                                                                          <  2:127407/100‑1 (MQ=255)
        tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCtt                                                                                      >  2:744320/1‑100 (MQ=255)
        tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCtt                                                                                      >  2:399023/1‑100 (MQ=255)
         cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTg                                                                                     <  2:1542165/100‑1 (MQ=255)
         cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTg                                                                                     >  2:318816/1‑100 (MQ=255)
           cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCg                                                                                   <  2:1799895/100‑1 (MQ=255)
           cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCg                                                                                   <  2:424554/100‑1 (MQ=255)
            ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCgg                                                                                  <  2:1090450/100‑1 (MQ=255)
             gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGa                                                                                 <  2:641320/100‑1 (MQ=255)
             gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGa                                                                                 <  2:1147687/100‑1 (MQ=255)
              tAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGaa                                                                                <  2:731841/100‑1 (MQ=255)
               aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAg                                                                               <  1:1094323/100‑1 (MQ=255)
                aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGt                                                                              <  1:1594896/100‑1 (MQ=255)
                aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGt                                                                              <  2:12307/100‑1 (MQ=255)
                   gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTa                                                                           >  2:961816/1‑100 (MQ=255)
                    cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTAt                                                                          >  1:187556/1‑100 (MQ=255)
                    cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTAt                                                                          >  2:174368/1‑100 (MQ=255)
                    cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTAt                                                                          >  2:281211/1‑100 (MQ=255)
                    cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTAt                                                                          <  1:1265935/100‑1 (MQ=255)
                        aGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTg                                                                       >  1:538351/1‑99 (MQ=255)
                         gCCTCGATGCGTTATGTGTTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                     <  1:844829/100‑1 (MQ=255)
                         gCCTCGAGGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                     <  1:769633/100‑1 (MQ=255)
                          ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCg                                                                    <  1:1377772/100‑1 (MQ=255)
                            tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGa                                                                   <  1:1587504/99‑1 (MQ=255)
                            tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc                                                                     <  1:1125923/97‑1 (MQ=255)
                             cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATg                                                                 <  2:1578666/100‑1 (MQ=255)
                             cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATg                                                                 >  1:463982/1‑100 (MQ=255)
                                   gTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                                                           >  1:228887/1‑100 (MQ=255)
                                   atcttgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                                                           <  1:1157452/96‑1 (MQ=255)
                                    ttATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGTGGAAGTTTATCTGGCGATGACCggtg                                                          <  2:1108797/100‑1 (MQ=255)
                                       tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtagat                                                       >  2:1157463/1‑96 (MQ=255)
                                       tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                                                       <  1:1310095/100‑1 (MQ=255)
                                       tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                                                       <  1:1038996/100‑1 (MQ=255)
                                        gtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCa                                                      >  1:1745022/1‑100 (MQ=255)
                                             cGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt                                                 >  1:1817381/1‑100 (MQ=255)
                                             agatACTAAATAGATAACAGTAAACGAACGCTGCACGGGGGATTACTAGATGTGCAGATCCTTGCGGAAGTTTATCTGGCGATGATCGGTGATCACACGt                                                 >  1:1317753/4‑100 (MQ=255)
                                               cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                               <  1:318814/100‑1 (MQ=255)
                                                 aCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                             <  2:399470/100‑1 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                            <  1:1370043/100‑1 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                            <  1:309891/100‑1 (MQ=255)
                                                   gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                            >  1:1009898/1‑99 (MQ=255)
                                                        aGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCtttt                                      >  1:222051/1‑100 (MQ=255)
                                                         gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                     <  1:113248/100‑1 (MQ=255)
                                                          aTAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc                                    >  2:521671/1‑100 (MQ=255)
                                                          aTAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc                                    >  1:1078905/1‑100 (MQ=255)
                                                           tAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg                                   >  2:1194275/1‑100 (MQ=255)
                                                            aaCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGa                                  <  2:1533046/100‑1 (MQ=255)
                                                             aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                 <  1:983170/100‑1 (MQ=255)
                                                             aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                 <  2:828511/100‑1 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                >  1:261400/1‑100 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                >  1:1082404/1‑100 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                >  1:1301123/1‑100 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                >  1:523202/1‑100 (MQ=255)
                                                                gTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa                              <  1:742117/100‑1 (MQ=255)
                                                                gTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGATCAAACGTCGATGGCTTTTGCGATGGa                              >  2:61689/1‑100 (MQ=255)
                                                                    acgaacgCTGCACGGGGCATTTCTCTATGTCCAGATTCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAgg                           <  2:191381/99‑1 (MQ=255)
                                                                      taacgCTGCACGGGTCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGga                          <  2:1427431/97‑1 (MQ=255)
                                                                       aacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGTGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagag                       <  1:1468225/100‑1 (MQ=255)
                                                                         cgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGac                     <  2:926779/100‑1 (MQ=255)
                                                                          gCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGac                     <  2:624805/99‑1 (MQ=255)
                                                                            tGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca                  >  1:1431924/1‑100 (MQ=255)
                                                                            tGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca                  <  1:349840/100‑1 (MQ=255)
                                                                               aCGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca               >  1:13459/1‑100 (MQ=255)
                                                                               aCGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                <  1:1739537/99‑1 (MQ=255)
                                                                                cGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                <  1:474983/98‑1 (MQ=255)
                                                                                       ttACTCGATGTCCAGATCCTTGCGGAATTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAg       <  1:1135135/100‑1 (MQ=255)
                                                                                        tACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg      <  2:113949/100‑1 (MQ=255)
                                                                                            cGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGaa  >  2:44396/1‑100 (MQ=255)
                                                                                            cGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGaa  >  1:1414314/1‑100 (MQ=255)
                                                                                                 |                                                                                              
AAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAA  >  NC_000913/236472‑236663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑