Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,755,639 G→A intergenic (‑498/‑59) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,755,6390GA95.2% 261.3 / ‑0.9 83intergenic (‑498/‑59)ydhZ/pykFuncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (46/33);  total (47/36)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA  >  NC_000913/1755540‑1755735
                                                                                                   |                                                                                                
ggCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCgg                                                                                                  >  1:1250929/1‑100 (MQ=255)
   tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAg                                                                                               <  2:679883/100‑1 (MQ=255)
    gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGt                                                                                              <  2:1097744/100‑1 (MQ=255)
     agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTg                                                                                             >  2:1554790/1‑100 (MQ=255)
      gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                            >  2:114203/1‑100 (MQ=255)
      gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                            >  2:841617/1‑100 (MQ=255)
      gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                            >  2:1373487/1‑100 (MQ=255)
      gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                            <  1:902241/100‑1 (MQ=255)
        cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                          >  2:290918/1‑100 (MQ=255)
          caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCGCTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                        >  1:986121/1‑100 (MQ=255)
          caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCAATTTCATAAAACCGAATTAGCTATCAAGCAGTGCGccc                                                                                        >  2:53841/1‑100 (MQ=255)
           gaGCACACATTCCTCTGCACGCTTTTTTTATTTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                       >  1:406592/2‑100 (MQ=255)
            aGCACACATTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAGCGAGGCACCACCACTTTCGTTATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                       >  1:775281/1‑99 (MQ=255)
            aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                      >  2:799338/1‑100 (MQ=255)
            aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                      >  2:809190/1‑100 (MQ=255)
             gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGa                                                                                     <  2:115979/100‑1 (MQ=255)
                cacaTTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAAAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACCGTGCGCCCAGaaa                                                                                   >  1:1511621/1‑99 (MQ=255)
                cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCAACACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                  <  2:514913/100‑1 (MQ=255)
                  caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCa                                                                                >  1:1605743/1‑100 (MQ=255)
                    ttCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAg                                                                              <  2:305115/100‑1 (MQ=255)
                      ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGTGCCCAGAAAGCAAGtt                                                                            >  2:866719/1‑100 (MQ=255)
                      ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                            >  2:39817/1‑100 (MQ=255)
                       ctctGCACGCTTTTTTTTTTTTTTCTATCCTTAGAGCGGGGCACCACCACTTTCGTAATACCGGATTCGCTTTTCGACAGTGCGCCTAGCAATCAAGttt                                                                           >  1:35481/1‑100 (MQ=255)
                        tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtc                                                                          <  2:513157/100‑1 (MQ=255)
                        tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtc                                                                          <  2:1722233/100‑1 (MQ=255)
                         ctGCACGCTTTTTTTTTTTTTCTTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGACAGCAAGTTtc                                                                          >  1:1416150/1‑99 (MQ=255)
                           gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  2:986129/100‑1 (MQ=255)
                           gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  1:629409/100‑1 (MQ=255)
                           gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCACTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       >  2:1074708/1‑100 (MQ=255)
                            cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                      <  2:1585951/100‑1 (MQ=255)
                            cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                      >  1:1306173/1‑100 (MQ=255)
                            cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                      >  2:883391/1‑100 (MQ=255)
                            cACGCTTTTTCGAGGGTACCTATCCTTAGATCGACGAACCACAACTTTCGTAATACCGTATGCGCTTTCCGGCAGTGCGTCCAGTAAGCAAGTTTCAcc                                                                       <  1:294941/99‑1 (MQ=255)
                                cTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                  <  1:982301/100‑1 (MQ=255)
                                  ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCtt                                                                >  2:137180/1‑100 (MQ=255)
                                   tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                               <  2:1732148/100‑1 (MQ=255)
                                   tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                               <  1:839175/100‑1 (MQ=255)
                                     tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc                                                             <  1:252237/100‑1 (MQ=255)
                                        aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                          <  1:649763/100‑1 (MQ=255)
                                        aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                          <  2:597174/100‑1 (MQ=255)
                                            cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                      <  2:468789/100‑1 (MQ=255)
                                              ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    <  2:1087180/100‑1 (MQ=255)
                                              ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    >  1:1716121/1‑100 (MQ=255)
                                                tATCCTTAGAGCGATGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                  <  2:851999/100‑1 (MQ=255)
                                                tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                  >  2:390828/1‑100 (MQ=255)
                                                 aTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagact                                                 <  2:1512284/100‑1 (MQ=255)
                                                   ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTGCTCCCATCCTTCTCAACTTAaagactaa                                               >  1:692851/1‑100 (MQ=255)
                                                    cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                              >  1:1392238/1‑100 (MQ=255)
                                                    cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                              >  2:1515479/1‑100 (MQ=255)
                                                      tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                            <  2:1219892/100‑1 (MQ=255)
                                                      cagatCGAGGCTCCACCACTTTAATAATAGCGGATACGCTTTCCGACAGTGCGCCTAGAAAGCAAGTTTCTCCCTTCCTTCTCAGCGTAaagaccaag                                              >  1:342762/2‑98 (MQ=255)
                                                           cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                       >  1:1033219/1‑100 (MQ=255)
                                                           cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                       <  1:1722221/100‑1 (MQ=255)
                                                           cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                       <  2:406594/100‑1 (MQ=255)
                                                            gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                      >  1:17149/1‑100 (MQ=255)
                                                              ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                    <  2:1250940/100‑1 (MQ=255)
                                                              ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                    >  1:241608/1‑100 (MQ=255)
                                                               gCACCACCACTTTCGTAATACCGTATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaa                                   <  2:373996/100‑1 (MQ=255)
                                                                caccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAACGACTAAGACTGTCATGaaa                                  >  1:1637103/1‑100 (MQ=255)
                                                                 accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                 >  2:1551423/1‑100 (MQ=255)
                                                                     ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                             <  1:1552476/100‑1 (MQ=255)
                                                                     ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                             <  1:39817/100‑1 (MQ=255)
                                                                          ttCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaaa                        >  1:865844/1‑100 (MQ=255)
                                                                           tCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAt                       >  2:640456/1‑100 (MQ=255)
                                                                             gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAtt                      >  2:1372746/1‑99 (MQ=255)
                                                                             gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                     >  2:707147/1‑100 (MQ=255)
                                                                             gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                     <  2:670620/100‑1 (MQ=255)
                                                                             gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                     >  2:1116496/1‑100 (MQ=255)
                                                                              tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt                    >  2:1027022/1‑100 (MQ=255)
                                                                               aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                   >  1:1145070/1‑100 (MQ=255)
                                                                               aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                   <  1:290916/100‑1 (MQ=255)
                                                                               aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTATAATGAAAAAGACCAAAATTGtt                   <  1:483086/100‑1 (MQ=255)
                                                                                aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                  >  2:1155602/1‑100 (MQ=255)
                                                                                 tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                 <  1:1754035/100‑1 (MQ=255)
                                                                                    cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCCAGTTTCTCCAATCCTTCTCATCTTAAAGTCTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc                <  1:1163277/98‑1 (MQ=255)
                                                                                      gATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCa            >  2:1064636/1‑100 (MQ=255)
                                                                                          cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc          >  1:503/1‑98 (MQ=255)
                                                                                          cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg        >  1:1653571/1‑100 (MQ=255)
                                                                                          cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg        >  1:1629413/1‑100 (MQ=255)
                                                                                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      >  2:581522/1‑100 (MQ=255)
                                                                                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      <  1:1530933/100‑1 (MQ=255)
                                                                                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      >  1:133590/1‑100 (MQ=255)
                                                                                            cTTTCCGACAGTGCGCCCAAAAAGCAATTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAATACTAAAATTGTTTGCACAATCGGAc      <  1:309973/100‑1 (MQ=255)
                                                                                                ccGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGaa  >  2:1529397/1‑100 (MQ=255)
                                                                                                   |                                                                                                
GGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA  >  NC_000913/1755540‑1755735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑