| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,942,523 | T→C | T21A (ACA→GCA) | znuA ← | zinc ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,942,523 | 0 | T | C | 92.4% | 198.9 / 4.3 | 66 | T21A (ACA→GCA) | znuA | zinc ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (28/33); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGT > NC_000913/1942427‑1942619 | gTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGtgctgc < 2:720546/100‑1 (MQ=255)gTAAAACCTCCGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGATTAAGCGAAGCGACAACGGCAGCATCTGCGGCCTGTGTTGc > 2:81959/1‑100 (MQ=255) aaaaCCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGtgctgc > 1:86295/1‑98 (MQ=255) aaaaCCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCAc > 1:1081029/1‑100 (MQ=255) ctctGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTccc > 2:1072419/1‑100 (MQ=255) ctgtttctgttACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCa > 2:1265642/1‑100 (MQ=255) tgtttctgttACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCa < 2:384278/99‑1 (MQ=255) gtttctgttACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCaga > 2:1384117/1‑100 (MQ=255) gtttctgttACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCaga > 2:1567037/1‑100 (MQ=255) ttctgttACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCagaga < 2:1405729/100‑1 (MQ=255) tACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAgcggcg < 1:1116900/100‑1 (MQ=255) tACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAgcggcg < 1:846106/100‑1 (MQ=255) tACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAgcggcg < 1:1623403/100‑1 (MQ=255) ccccATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAATCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGa < 1:361413/100‑1 (MQ=255) cATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATaa > 2:295524/1‑100 (MQ=255) aTCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAAt < 2:1150240/100‑1 (MQ=255) tCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAAtg > 2:105276/1‑100 (MQ=255) aGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAAtgct < 1:564436/100‑1 (MQ=255) cAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAAtgctgc > 2:1760791/1‑100 (MQ=255) aaTTGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCg < 1:1604471/100‑1 (MQ=255) aaTGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCg < 2:224245/100‑1 (MQ=255) ggCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGaaa > 2:689338/1‑100 (MQ=255) cAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCAGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAaag > 1:1158866/1‑100 (MQ=255) aGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAgaga < 2:1819659/100‑1 (MQ=255) aaGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAaagaag < 2:558459/100‑1 (MQ=255) gtgATGAACCCAGATGTTTTAAGTGAAGCGACAACGGCGGTATCTGCGGCCTGTGCTGAACCTCCCCAGGGAGCGGCGGATAATGCTGCGAAaagaag < 2:725885/96‑1 (MQ=255) gCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCg < 2:268978/100‑1 (MQ=255) cGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCAGCCTGTGTTGCACCTCCCCAGAGAGCAACAAATAATGCAGCGAAAAGAAGCg > 2:1536669/1‑99 (MQ=255) gATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGt > 2:1542604/1‑99 (MQ=255) aTGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGttt > 1:1044417/1‑100 (MQ=255) tGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGtttt > 2:1620586/1‑100 (MQ=255) aaCCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGtttttt > 2:1148554/1‑100 (MQ=255) aCCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGATAGCGGCGGATAATGCTGCGAAAAGAAGCGttttttt > 2:1732422/1‑100 (MQ=255) aCCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGttttttt > 2:690427/1‑100 (MQ=255) aCCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCGCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGtttttt > 2:989973/1‑99 (MQ=255) cccAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCCCCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTa > 2:1681852/1‑100 (MQ=255) cccAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTa < 1:1298411/100‑1 (MQ=255) cAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATg < 2:1093095/100‑1 (MQ=255) cAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATg < 1:295522/100‑1 (MQ=255) cAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGAACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAGAGAAGCGTTTTTTTATg > 1:1566667/1‑100 (MQ=255) cAACGGGTTTAAGCGAAACGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCCGATAATGCTGCGAAAAGAAGCGTTTTTTTATg > 1:581417/1‑100 (MQ=255) gggTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAAc < 1:619476/100‑1 (MQ=255) ggTTTAAGCGAAGCGACAACGACGGCATCTGCGGCCTGTGCTGCACCTCACCAGAGAGCGGCGGAAAATGCTGCGAAAAGAAGCGTTTTTTTATGTAAc > 1:779280/1‑99 (MQ=255) gTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACAt > 2:893501/1‑100 (MQ=255) tttAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATa > 2:78407/1‑100 (MQ=255) aagcgaagcgACAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATg < 1:486272/100‑1 (MQ=255) agcgaagcgaCAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGc < 1:1713380/100‑1 (MQ=255) gcgaagcgaCAACGGCGACATCTGCGGCCTGTGCTGCACCTCCCCCGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATACg > 1:300804/1‑100 (MQ=255) cgaagcgaCAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGCAACATAATGCGa < 2:1191578/100‑1 (MQ=255) gaagcgaCAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGAc > 1:1339385/1‑100 (MQ=255) aagcgaCAACGGTGGCATCTGCGGCCTGTGCTGCACATCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGAcc < 2:365751/100‑1 (MQ=255) agcgaCAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCa > 2:1082303/1‑100 (MQ=255) cgaCAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCaat < 2:107652/100‑1 (MQ=255) gaCAACGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCaata > 2:137835/1‑100 (MQ=255) aCGGCGGCATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCg < 2:926682/100‑1 (MQ=255) ggcggcATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTa < 1:1265631/100‑1 (MQ=255) gcggcATCTGCGGCCTGTGCTGCATCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTaa < 2:1093113/100‑1 (MQ=255) ggcATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGTGGCGGATAATGCTGCGAAAAGATGCGTTTTTTTATGTAACATAATGCGACCAATAAGCGTaaaa < 1:560933/100‑3 (MQ=255) ggcATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATg < 1:818342/100‑1 (MQ=255) ggcATCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATg < 1:1107061/100‑1 (MQ=255) gcATCTGCGGCCTGTGTTGCACCGCCCAAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTCTGTAACATAATGCGACCAATCATCGTAATGa < 1:81959/100‑1 (MQ=255) aTCTGCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAat < 2:1339395/100‑1 (MQ=255) tGCGGCGTGTGTTGCATCTCCCCAGTTAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATg < 1:774374/100‑1 (MQ=255) gCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATga > 2:854567/1‑100 (MQ=255) gCGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATTATCGTAATGAata > 1:1585044/1‑97 (MQ=255) cGGCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATgag < 1:1344099/100‑1 (MQ=255) gCCTGTGCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGaa < 2:1081039/100‑1 (MQ=255) tgtgCTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAg < 1:787728/98‑1 (MQ=255) gtgcTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAgtgt > 1:1362521/1‑100 (MQ=255) | GTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGT > NC_000913/1942427‑1942619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |