| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,353,249 | T→C | H201H (CAT→CAC) | glpA → | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,353,249 | 0 | T | C | 98.2% | 185.6 / ‑4.2 | 54 | H201H (CAT→CAC) | glpA | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (33/20); total (34/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01 | |||||||||||
TACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTG > NC_000913/2353153‑2353345 | tACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCa > 2:1742060/1‑100 (MQ=255) ccGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCAcc < 2:1081211/100‑1 (MQ=255) cGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCt > 1:1229758/1‑100 (MQ=255) gCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTg > 2:410617/1‑100 (MQ=255) gCTCATGAAGTCACGGGGCCGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTg > 2:759035/1‑100 (MQ=255) tCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCATCGGCGAAACTCAGGCCCTTCACGCACCTGTc > 2:467803/1‑100 (MQ=255) cATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCg > 2:52650/1‑100 (MQ=255) tGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGACGAAACTCAGGCCCTTCACGCACCTGTCGTg > 2:1135771/1‑100 (MQ=255) aaGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGt < 1:433043/100‑1 (MQ=255) aGTCACGGAGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGtt > 2:1063623/1‑100 (MQ=255) gTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTa > 1:1387159/1‑100 (MQ=255) ggCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGc < 2:1797544/100‑1 (MQ=255) gCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCt > 1:731215/1‑100 (MQ=255) tGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCATCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGTCGCTgg < 1:467801/100‑1 (MQ=255) gATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTggg > 1:155831/1‑100 (MQ=255) ttCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCTCTTCACTCACCTGTCGTGGTTAATGCCGCTgggg < 1:1575328/99‑2 (MQ=255) ttCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGAt > 2:654837/1‑100 (MQ=255) ttCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGAt < 1:759031/100‑1 (MQ=255) aaGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTgggg < 2:246290/100‑1 (MQ=255) aaGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTggg < 2:169941/99‑1 (MQ=255) aGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTggggg < 1:1356150/100‑1 (MQ=255) gcgACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAac < 1:505818/100‑1 (MQ=255) ngACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAaca > 2:66991/2‑100 (MQ=255) ctaCGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAac < 2:628577/97‑1 (MQ=255) cgACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAaca > 1:863611/1‑100 (MQ=255) cgACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAaca > 1:863442/1‑100 (MQ=255) aCGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAAcaca < 2:1214150/100‑1 (MQ=255) ggTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACAtt < 1:410615/100‑1 (MQ=255) gtgCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGcc > 2:1003502/1‑100 (MQ=255) gCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGa > 2:953770/1‑100 (MQ=255) ggTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAat < 1:560297/100‑1 (MQ=255) cGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGAGGGCAACACATTGCCGAATATGCCg > 2:509547/1‑100 (MQ=255) gtgtACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATTCCGCTGGGATCTGGGGGCAACACATTGAAGAATATGCCGa > 2:569864/1‑100 (MQ=255) tAACCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGAAAAAAAATTCCCAAAAATGccaacctcgca > 2:1216260/1‑91 (MQ=255) aaCCATCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCa > 2:831081/1‑100 (MQ=255) aTCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCg > 1:1158151/1‑100 (MQ=255) aTCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCg < 1:252058/100‑1 (MQ=255) ctcACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCa < 1:415800/100‑1 (MQ=255) cACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATg > 1:900999/1‑100 (MQ=255) ccGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGtt > 2:1253330/1‑100 (MQ=255) ccGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGtt > 1:1604172/1‑100 (MQ=255) cGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTc > 2:1662784/1‑100 (MQ=255) gCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGTGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTcc < 1:395367/99‑1 (MQ=255) aaaCTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGc < 2:1773369/100‑1 (MQ=255) aaCTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCg > 2:986585/1‑100 (MQ=255) aaCTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCg > 2:1159667/1‑100 (MQ=255) cTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGaa > 1:754782/1‑100 (MQ=255) tCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGaaa > 2:981136/1‑100 (MQ=255) cAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGaaa < 2:450723/99‑1 (MQ=255) ggCCCTTCACGCACCTGTCGTGGTTAATGACGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTCCGCATTCGCATGTTCCCGGCGAAAGGa > 1:253600/1‑100 (MQ=255) ccTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCg > 2:824666/1‑100 (MQ=255) tCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCgctg > 2:1567578/1‑100 (MQ=255) tCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCgctg > 2:1294882/1‑100 (MQ=255) tCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCgctg < 1:96502/100‑1 (MQ=255) | TACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTG > NC_000913/2353153‑2353345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |