| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,362,984 | A→G | I217T (ATC→ACC) | yfaY ← | inactive PncC family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,362,984 | 0 | A | G | 98.1% | 173.6 / ‑4.3 | 53 | I217T (ATC→ACC) | yfaY | inactive PncC family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (27/24); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
CACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTA > NC_000913/2362893‑2363079 | cACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCAtt > 1:1704451/1‑100 (MQ=255) aCGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTg < 2:583642/100‑1 (MQ=255) tGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGc > 1:1218139/1‑100 (MQ=255) tCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGa > 1:1424534/1‑100 (MQ=255) tCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGa > 1:718727/1‑100 (MQ=255) cGGCAACACGTTTAACATCCAGCCACAGTTTTTCGATGGCCTGTTGCTCGGTTGCCGGGCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGc < 1:1432527/96‑1 (MQ=255) ccgatctgTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGc < 2:1484584/93‑1 (MQ=255) cAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGcc < 1:163600/100‑1 (MQ=255) gTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGcagatcgg > 1:1484573/1‑93 (MQ=255) gTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGt > 1:489895/1‑100 (MQ=255) gTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGt < 2:1537676/100‑1 (MQ=255) gTTTAACATCCAGCCACAGTTTTTCCATCGCCGGTGGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGt < 2:891354/100‑1 (MQ=255) tttAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGtt < 1:1027198/100‑1 (MQ=255) ttAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTa > 1:201774/1‑100 (MQ=255) tAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTAc < 1:1643119/100‑1 (MQ=255) aaCATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCCTCCCGGTGCGGCGAGATTCAGATCGATGGTAGTCCTGGAGGAGCGATAGCCCATTGTTACg > 1:1589868/1‑100 (MQ=255) cATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGcc > 2:1411951/1‑100 (MQ=255) cATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGcc > 2:1271272/1‑100 (MQ=255) tCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCcg > 2:819836/1‑100 (MQ=255) tCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCcg > 1:305322/1‑100 (MQ=255) ccAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCcg > 1:1158631/1‑99 (MQ=255) aGCCACATTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCcggc < 2:1168907/99‑1 (MQ=255) gCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCcggcgg < 2:660794/100‑1 (MQ=255) ccACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCggcggc > 2:1563127/1‑100 (MQ=255) ccACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCggcggc < 2:1218150/100‑1 (MQ=255) ccACAGTGTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTGCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCggcggc < 2:1798797/100‑1 (MQ=255) gTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGTGGAGCGAAAGCCTATTGTTATGCCCGGCGGCAGt > 1:1062502/1‑98 (MQ=255) tttttCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGt < 2:1344236/100‑1 (MQ=255) tCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTagag < 2:1424545/100‑1 (MQ=255) gCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCa > 2:1180289/1‑100 (MQ=255) gCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTtg > 1:1002198/1‑100 (MQ=255) cTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGAGTCCAGGCTTtgt > 2:993770/1‑100 (MQ=255) tCGCTTGCCTGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTtgtg < 2:625276/100‑1 (MQ=255) gCTTGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGcc < 1:723687/100‑1 (MQ=255) ttGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAg < 2:1442154/100‑1 (MQ=255) ttGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAg < 2:718731/100‑1 (MQ=255) tGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGGCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGa > 1:437181/1‑100 (MQ=255) tGCCGGTCCGGTGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCTCGACGGCAGTTGTAGAATGTCCAGGCTTTGTGCCAGa > 2:1498470/1‑100 (MQ=255) ccggtccggtGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATc > 1:1363078/1‑100 (MQ=255) ggtccggtGAGTTTCAGTTCGATGGTAGGCATTGATGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGc < 2:497321/100‑1 (MQ=255) gtccggtGAGTTTCATTTCGATTTTATGCATTGAGTATCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGc < 2:419988/99‑1 (MQ=255) ccggtGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAATGTCCAGGCTTTGAGCAAGATCGCtct > 2:142342/1‑98 (MQ=255) tgtgtgtTTCAGTTCGATTGTAGGTATTGAGGAGCGATAGCCCATTGTAACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTcc < 2:1132587/99‑1 (MQ=255) ggtGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTcc < 2:608548/100‑1 (MQ=255) ggtGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTcc < 1:399432/100‑1 (MQ=255) ggtGAGTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTc < 2:295304/99‑1 (MQ=255) gTTTCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACg > 2:1086692/1‑100 (MQ=255) gTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACg > 2:638102/1‑100 (MQ=255) ttCAGTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGACTTTGTGCCAGATCGCTTTCCGAACGAc > 2:599611/1‑100 (MQ=255) gTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCaaa > 2:1460300/1‑100 (MQ=255) gTTCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCaaa > 2:1417082/1‑100 (MQ=255) tCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAag < 2:656440/100‑1 (MQ=255) tCGATGGTAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAag > 1:7426/1‑100 (MQ=255) gatggtAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAagta < 1:1615326/100‑1 (MQ=255) | CACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTA > NC_000913/2362893‑2363079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |