| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,553,210 | A→G | K287E (AAA→GAA) | amiA → | N‑acetylmuramoyl‑l‑alanine amidase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,553,210 | 0 | A | G | 97.2% | 243.2 / NA | 72 | K287E (AAA→GAA) | amiA | N‑acetylmuramoyl‑l‑alanine amidase I |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (22/47); minor base T (0/2); total (23/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATG > NC_000913/2553115‑2553306 | tAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGgaaaa > 1:674008/1‑100 (MQ=255)tAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGgaaaa < 2:1399955/100‑1 (MQ=255) ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCTACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGc < 2:1282355/100‑1 (MQ=255) ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGc < 1:259206/100‑1 (MQ=255) gCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTGCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCg < 1:1121259/100‑1 (MQ=255) gCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCg > 2:934616/1‑100 (MQ=255) cggcggCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATa > 2:1150008/1‑100 (MQ=255) gcggcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAg > 2:1753191/1‑100 (MQ=255) cggcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGt < 2:1428292/100‑1 (MQ=255) gcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTa < 1:1007677/100‑1 (MQ=255) cgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTAt < 1:113210/100‑1 (MQ=255) gTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACGACCAGAAAGCACATTCGGAAAAGCGATAAGTTATg < 1:1439565/100‑1 (MQ=255) gTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATg > 2:385467/1‑100 (MQ=255) ttCGTCAGAAAATCGCCACAGCGATTGCTGAAGACGTGATCAGTTATTTCCACTGGTTCGACAACCAGAACGCACATTCGGAAAAGCGATAAGTTATGaa > 1:1092957/1‑100 (MQ=255) gTCAGAAAATCGCCACAGCGATTTCTGAAGGCTTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCATATTCGTTAAAGCGATAAGCTATGaaa < 1:440420/98‑1 (MQ=255) gTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAAcc < 2:1251315/100‑1 (MQ=255) cAGAAAATCGCCACATCGATTGCGGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAAcc < 2:1665873/98‑1 (MQ=255) aTCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGcac < 1:1395297/100‑1 (MQ=255) tCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGcaca < 1:929586/100‑1 (MQ=255) gCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACAcc < 2:501520/100‑1 (MQ=255) ccACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCa < 1:385465/100‑1 (MQ=255) cacaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAg < 2:1374137/100‑1 (MQ=255) cacaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAg > 1:766177/1‑100 (MQ=255) caGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGt < 2:1173226/100‑1 (MQ=255) aGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGtt < 2:46588/100‑1 (MQ=255) gCGATTTCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGAAAAACAGAAAGCACATTAGGAAAGGAGTTAAGTTATGAAACCCGACGCACGCCAgatga > 1:1197873/1‑96 (MQ=255) gCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGtt > 1:1785479/1‑99 (MQ=255) cGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTaa < 1:847938/100‑1 (MQ=255) cGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTaa > 2:695111/1‑100 (MQ=255) ttGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTaa < 2:1606132/97‑1 (MQ=255) cTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAg > 1:566101/1‑98 (MQ=255) tGAAGGCGTGATCAGTTATTTCCACTGTTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGttt < 1:1422781/100‑1 (MQ=255) gAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTc > 1:742855/1‑100 (MQ=255) gAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTc > 1:742773/1‑100 (MQ=255) aaGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCt < 2:941930/100‑1 (MQ=255) ggCGTGATCAGTTATTTCCACTGGTTCGACTACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGc < 2:1455313/100‑1 (MQ=255) gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt < 1:381123/100‑1 (MQ=255) gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt < 1:334867/100‑1 (MQ=255) gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt < 1:187958/100‑1 (MQ=255) tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa < 1:1336463/100‑1 (MQ=255) tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa < 1:799738/100‑1 (MQ=255) tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa < 1:63220/100‑1 (MQ=255) gATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAAc > 1:216284/1‑100 (MQ=255) tCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCt < 1:847310/100‑1 (MQ=255) aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCtt < 1:1213073/99‑1 (MQ=255) aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTc > 1:1257461/1‑100 (MQ=255) tATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAgg < 2:149808/100‑1 (MQ=255) tATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAgg < 1:934610/100‑1 (MQ=255) aTTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGa > 2:503048/1‑100 (MQ=255) tttCCACTGGTTCGACAACCAGAAAGCACACTCGGAAAAGCGCTAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCATCAGGAt > 2:194815/1‑100 (MQ=255) tCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATAc < 1:1758603/100‑1 (MQ=255) tCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATAc < 1:1037555/100‑1 (MQ=255) ccACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACg > 2:1539583/1‑100 (MQ=255) ccACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACg > 1:1136441/1‑100 (MQ=255) ggTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg < 2:1279872/100‑1 (MQ=255) ggTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg > 1:575289/1‑100 (MQ=255) ggTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg < 1:1320696/100‑1 (MQ=255) ttCGACAACCAGAAAGCACATTCGTATAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc < 2:33029/100‑1 (MQ=255) ttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc < 1:136774/100‑1 (MQ=255) ttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc < 1:565840/100‑1 (MQ=255) tCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc < 1:733053/99‑1 (MQ=255) gACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagc > 1:1409495/1‑100 (MQ=255) cAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAGACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcaggag > 1:1116983/1‑100 (MQ=255) aaCCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCagcagc < 1:908224/100‑1 (MQ=255) aCCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:314974/100‑1 (MQ=255) ccAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg < 1:188216/100‑1 (MQ=255) ccAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg > 2:862182/1‑100 (MQ=255) ccAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg < 2:89885/100‑1 (MQ=255) aGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAc > 1:1310867/1‑100 (MQ=255) aGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAc < 1:1694258/100‑1 (MQ=255) gAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCTGACGCACACCAGGTTAATCAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAcc < 1:1000932/100‑1 (MQ=255) aaGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccgc < 2:766182/100‑1 (MQ=255) aaGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccgc < 1:800300/100‑1 (MQ=255) tCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATg < 1:1586797/100‑1 (MQ=255) | TAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATG > NC_000913/2553115‑2553306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |