| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,643,753 | G→A | G177G (GGC→GGT) | rlmN ← | dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,643,753 | 0 | G | A | 98.5% | 216.4 / ‑5.0 | 66 | G177G (GGC→GGT) | rlmN | dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (31/33); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGAC > NC_000913/2643656‑2643849 | gCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGTTTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccatacc < 2:189807/100‑1 (MQ=255)gCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccatacc < 2:920878/100‑1 (MQ=255) cGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccataccc > 2:285601/1‑100 (MQ=255) gACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCcatc < 1:1278568/100‑1 (MQ=255) cgcgACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCcatcat > 1:805139/1‑100 (MQ=255) gcgACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCatcatc < 2:992246/100‑1 (MQ=255) gACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCAc < 2:981196/100‑1 (MQ=255) ttAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGt > 2:642428/1‑100 (MQ=255) tAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGATGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGtt > 2:146012/1‑100 (MQ=255) gACAGGTCAAAATCTAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAg < 2:1576992/100‑1 (MQ=255) gACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAg > 2:1667186/1‑100 (MQ=255) gACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAg > 1:815770/1‑100 (MQ=255) aGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGa > 2:181709/1‑100 (MQ=255) ggCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGAt > 2:651993/1‑100 (MQ=255) ccAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCg > 1:349091/1‑100 (MQ=255) ccAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCg < 2:273143/100‑1 (MQ=255) cAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCgg < 2:1218195/100‑1 (MQ=255) aaaCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCCTCATCACTACGTTAGTGATCGGAc > 1:468096/1‑100 (MQ=255) aaaCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGAc < 1:285599/100‑1 (MQ=255) aaaCCGAAATCATCAAGCATGATTTCCATCGCAGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGAc > 1:745144/1‑100 (MQ=255) aCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGc < 1:598865/100‑1 (MQ=255) cGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTg < 2:295288/100‑1 (MQ=255) cGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTg > 1:610922/1‑100 (MQ=255) aaTCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGAcc < 2:874702/100‑1 (MQ=255) aaTCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGAcc < 2:1487800/100‑1 (MQ=255) atcatcAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCg < 1:330531/100‑1 (MQ=255) catcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGt > 1:1410220/1‑100 (MQ=255) catcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGt > 1:1802450/1‑100 (MQ=255) atcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTg > 1:6988/1‑100 (MQ=255) ataaatcATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCAGACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTg < 2:326354/94‑1 (MQ=255) agcaccaTTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATTCCCACCCTCACTACGTTAGTGCTCGGAcgctgaccgctgac < 1:989677/92‑1 (MQ=255) aGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACtt < 1:394874/100‑1 (MQ=255) aTGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAATGATCGGACGCTGACCGGTGACtttt > 2:1153643/1‑99 (MQ=255) tGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgc < 1:20425/100‑1 (MQ=255) aTTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgctg < 2:951700/100‑1 (MQ=255) aTTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgctg < 1:1735953/100‑1 (MQ=255) aTTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgctg > 2:132008/1‑100 (MQ=255) tCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTgcgc > 1:1531964/1‑100 (MQ=255) tCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTgcgc > 2:1506608/1‑100 (MQ=255) cATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcg > 2:480608/1‑100 (MQ=255) aTCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcga > 1:917746/1‑100 (MQ=255) tCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGTCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgac < 2:456351/100‑1 (MQ=255) tCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgac < 2:1079243/100‑1 (MQ=255) gAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtttt > 1:1598939/1‑100 (MQ=255) gAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtttt > 2:546440/1‑100 (MQ=255) acgacgTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcg < 1:314699/100‑1 (MQ=255) acgacgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcg < 2:823610/100‑1 (MQ=255) cgacgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgc > 1:1679730/1‑100 (MQ=255) cgacgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgc > 2:404812/1‑100 (MQ=255) gacgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgcc > 1:151924/1‑100 (MQ=255) acgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgccg < 1:651989/100‑1 (MQ=255) cgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgccgc < 1:213455/100‑1 (MQ=255) cgTTGTTCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgccgc > 2:665644/1‑100 (MQ=255) ttgttCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCAc < 1:483240/100‑1 (MQ=255) tgttCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACg < 1:592109/100‑1 (MQ=255) ttCAGGTTGAGCAGCTGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGcc < 1:380802/100‑1 (MQ=255) ttCAGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGcc < 2:5087/100‑1 (MQ=255) aGGTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCcaca < 2:1817084/100‑1 (MQ=255) ggTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCacac > 1:1171660/1‑100 (MQ=255) ggTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCacac > 1:868990/1‑100 (MQ=255) gTTGAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACAcc < 1:352586/100‑1 (MQ=255) gAGCAGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTgg < 1:26765/100‑1 (MQ=255) agcagcGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGc > 2:88530/1‑100 (MQ=255) agcagcGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCAACGATTTTCGCCGCACGCCACACCTg > 2:262859/1‑98 (MQ=255) gcagcGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGcc < 2:1794234/100‑1 (MQ=255) gcagcGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGcc < 2:917752/100‑1 (MQ=255) agcGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGa < 1:1012429/100‑1 (MQ=255) gCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAAt > 2:1740243/1‑100 (MQ=255) cTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAAtt < 2:851994/100‑1 (MQ=255) gctcatacccatCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTcc < 2:1753065/97‑1 (MQ=255) gcccatacccatCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTcc > 1:477027/1‑100 (MQ=255) cccatacccatCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCg > 2:734098/1‑100 (MQ=255) cccatacccatCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCg > 2:734361/1‑100 (MQ=255) cccatacccatCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCg > 1:986462/1‑100 (MQ=255) catacccatCATCACTACGTTAGTGATCGTACGCTGACCGGTTACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGac < 2:488400/100‑1 (MQ=255) | GCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGAC > NC_000913/2643656‑2643849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |