Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,676,572	G→A	P85L (CCG→CTG)	yphD ←	putative sugar ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676,572	0	G	A	100.0%	186.9 / NA	56	P85L (CCG→CTG)	yphD	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/19);  total (37/19)

AGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCA  >  NC_000913/2676476‑2676665                                                                                                 |                                                                                              aGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCTGCCAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGcc                                                                                            >  1:1279285/1‑100 (MQ=255)    gcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCaa                                                                                         >  2:379756/1‑100 (MQ=255)      gcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACg                                                                                       >  1:69060/1‑100 (MQ=255)       ccTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAACATGCCAGGCTCACCGAGACAAAAGCCACCATCAGcacac                                                                                         >  1:1428043/1‑96 (MQ=255)         tAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTg                                                                                    <  1:424758/100‑1 (MQ=255)         tAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGACACACAGAGACAAAAGCAACCATCAGCCCAACGCTg                                                                                    >  2:572312/1‑100 (MQ=255)         tAACAGCAACACCAGCAGGCACGCCACCGCCAGCAGAACTTCAAATTGCAGCAAACATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCa                                                                                          >  1:1257547/1‑94 (MQ=255)           aCAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGAc                                                                                  <  1:1318881/100‑1 (MQ=255)             aGCAACACCCGCAGGCACGCAACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACa                                                                                 >  1:1779376/1‑99 (MQ=255)              ncAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGACACAAAAGCCACCATCAGCCCAACGCTGACATc                                                                               >  2:379101/2‑100 (MQ=255)               cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCa                                                                              >  2:759772/1‑100 (MQ=255)                  cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAAtt                                                                           <  1:379754/100‑1 (MQ=255)                  cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAAtt                                                                           <  1:323817/100‑1 (MQ=255)                      agcagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCAc                                                                       >  1:651955/1‑100 (MQ=255)                      agcagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGAACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCAc                                                                       <  2:12499/100‑1 (MQ=255)                         agGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTacac                                                                       >  1:1657610/1‑97 (MQ=255)                         agGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCgg                                                                    <  2:856748/100‑1 (MQ=255)                          gccACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGTCCAGCCCGGACAGCAATTTCACCGga                                                                   >  1:458661/3‑100 (MQ=255)                             aCGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGat                                                                >  2:559632/1‑100 (MQ=255)                              cGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGCCATCAATTTCACCGgagaca                                                               >  1:1017348/1‑98 (MQ=255)                              cGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGata                                                               <  1:1482305/100‑1 (MQ=255)                               gCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGAAGataa                                                              >  1:1497498/1‑100 (MQ=255)                                ccACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGataat                                                             >  2:1641574/1‑100 (MQ=255)                                ccACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATAAGACCAACGCTGACATCAATTTCACCGGAGataat                                                             >  2:953182/1‑100 (MQ=255)                                 cACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGataata                                                            <  1:709421/100‑1 (MQ=255)                                  aCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGAtaataa                                                           >  2:115954/1‑100 (MQ=255)                                       cAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGc                                                      >  2:1550794/1‑100 (MQ=255)                                        aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCg                                                     >  2:855549/1‑100 (MQ=255)                                           ggAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCa                                                  >  2:1545302/1‑100 (MQ=255)                                              aCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCg                                               <  1:839478/100‑1 (MQ=255)                                                 tCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGccc                                            <  2:730908/100‑1 (MQ=255)                                                 tCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGccc                                            <  2:700325/100‑1 (MQ=255)                                                        gcagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAggcgg                                      <  2:1279296/99‑1 (MQ=255)                                                         cagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCa                                    >  1:1045354/1‑100 (MQ=255)                                                         cagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAAGTTCACCAGAGAAAATAATCAGCGTCAGCGCCCAgccggc                                     >  1:618521/1‑99 (MQ=255)                                                          agcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCaa                                   >  2:913163/1‑100 (MQ=255)                                                                  tGCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAtg                           <  1:559628/100‑1 (MQ=255)                                                                   gCCAGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAtgg                          >  1:1579966/1‑100 (MQ=255)                                                                    cgaGGCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAtggt                         >  2:1228191/3‑100 (MQ=255)                                                                       ggCACACCGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTggc                      <  2:490352/100‑1 (MQ=255)                                                                       ggCACACCGAGACAAAAGCCACCAACAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAACGTCAACGCACCGGCGACAATCCCaaacg                          >  1:1445859/1‑93 (MQ=255)                                                                         cacaCCGAGACAAAAGCCACCATCAGCCCAGCGCTGACAGCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTggcgn                    <  1:379099/100‑2 (MQ=255)                                                                              ctataCAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATcg               <  2:1710785/96‑1 (MQ=255)                                                                              cGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATcg               <  2:1779389/100‑1 (MQ=255)                                                                              cGAGACAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATcg               >  1:1680400/1‑100 (MQ=255)                                                                               gagaCAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATcgc              >  1:1744362/1‑100 (MQ=255)                                                                               gagaCAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATcgc              >  1:1739136/1‑100 (MQ=255)                                                                               gagaCAAAAGACACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATcgc              >  1:1739122/1‑100 (MQ=255)                                                                                 gaCAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCgcgc            >  2:1017445/1‑100 (MQ=255)                                                                                 gaCAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCgcgc            >  2:1412142/1‑100 (MQ=255)                                                                                  aCAAAAGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgca           <  1:452235/100‑1 (MQ=255)                                                                                     aaaGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAACGGTGGCGGCATCGCgcagca        <  1:84232/100‑1 (MQ=255)                                                                                      aaGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcagc         >  1:837854/1‑98 (MQ=255)                                                                                       aGCCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGAAGCGGCATAGCGCAGCACg      >  2:453754/1‑100 (MQ=255)                                                                                         ccaccaTCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt    >  2:1100233/1‑100 (MQ=255)                                                                                           accaTCAGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCa  >  2:1579134/1‑100 (MQ=255)                                                                                                 |                                                                                              AGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCA  >  NC_000913/2676476‑2676665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑