| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,917,114 | G→A | L686L (TTG→TTA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,114 | 0 | G | A | 95.7% | 218.8 / ‑2.5 | 70 | L686L (TTG→TTA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (30/36); total (32/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGAT > NC_000913/2917020‑2917210 | ncacGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGa > 2:1587560/2‑100 (MQ=255)acacGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCAACTAACCTGAAACTTATCGGCGCATTACTGGa > 2:573849/1‑100 (MQ=255) cacGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGaa < 1:982587/100‑1 (MQ=255) cacGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGaa > 1:1723915/1‑100 (MQ=255) cTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGata < 2:274418/100‑1 (MQ=255) cTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGata > 1:731754/1‑100 (MQ=255) tGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGt > 1:1489006/1‑100 (MQ=255) gCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTg > 1:1368430/1‑100 (MQ=255) gTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAAc < 2:1344488/100‑1 (MQ=255) tAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCTCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACa < 2:502554/100‑1 (MQ=255) tAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACa > 2:212248/1‑100 (MQ=255) tAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACa < 2:319727/100‑1 (MQ=255) atcGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACAtg > 2:1139669/3‑100 (MQ=255) aCCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACAtg < 1:1167905/100‑1 (MQ=255) cggATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACAtgt > 2:1681639/3‑100 (MQ=255) ccGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACAtgt < 1:48502/100‑1 (MQ=255) cGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACAtgtg < 1:669605/100‑1 (MQ=255) gATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTTCAACATGTgg > 1:1704980/1‑100 (MQ=255) gATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTgg < 1:525244/100‑1 (MQ=255) aTGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGTTGATAACTCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGa < 2:610823/100‑1 (MQ=255) tGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCTGCGCATTACTGGAAGATATGGTGCAACAtgtg < 2:1724612/97‑1 (MQ=255) tAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGc < 2:1783304/100‑1 (MQ=255) aaGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCg < 1:704892/100‑1 (MQ=255) cTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATa > 2:1566794/1‑100 (MQ=255) tGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAg > 2:1081148/1‑100 (MQ=255) gCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCg > 2:281756/1‑100 (MQ=255) cAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCgg > 1:870607/1‑100 (MQ=255) cAATGACAGTCATGGCGATTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCgg > 2:1081966/1‑100 (MQ=255) aaTGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCggg < 1:8589/100‑1 (MQ=255) aGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAg > 1:467989/1‑100 (MQ=255) tCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAgg > 2:1125647/1‑99 (MQ=255) tGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGt < 1:943052/100‑1 (MQ=255) gCGGTTGATGACATCCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTg < 2:1735161/100‑1 (MQ=255) gCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTg < 1:1104692/100‑1 (MQ=255) gCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTg > 1:1442119/1‑100 (MQ=255) cGGTTGATGACAATCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGa < 2:723191/100‑1 (MQ=255) ggTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGaa > 1:926830/1‑100 (MQ=255) gTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAAc < 2:861756/100‑1 (MQ=255) ttGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACg > 1:230584/1‑100 (MQ=255) tgatgaCAACCCCGCTAACCTTAAACTTTTCGGCGCATTACTTGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACgg < 1:371402/100‑1 (MQ=255) tgatgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACgg > 1:190917/1‑100 (MQ=255) tgatgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACgg > 2:921125/1‑100 (MQ=255) gatgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACggg > 1:266951/1‑100 (MQ=255) atgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGc < 1:1309692/100‑1 (MQ=255) atgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTAGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGc < 1:1118403/100‑1 (MQ=255) tgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCg < 2:1363216/100‑1 (MQ=255) tgaCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCg > 1:400275/1‑100 (MQ=255) taactaCCCCGATATCCTGAACCTTTTCGTCGTTTAGATGGAAGACATTGTGCATTATGTGAATTTTTATGTTAGCTGGCATCAGGCGGTTGACCgggac < 1:1548154/95‑3 (MQ=255) aCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGaa < 2:1368440/100‑1 (MQ=255) aCAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGaa > 1:262089/1‑100 (MQ=255) cAACCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGaaa < 2:1398214/100‑1 (MQ=255) aaCCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAAc > 1:1424898/1‑100 (MQ=255) aaCCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAAc < 1:1673513/100‑1 (MQ=255) aCCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACa < 1:650978/100‑1 (MQ=255) aCCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACa > 1:1806923/1‑100 (MQ=255) aCCCCGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGACAGCGGGCATCAGGCGGTTGAACGGGCGAAACa < 2:1723928/100‑1 (MQ=255) cccGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGa < 2:927862/100‑1 (MQ=255) cGCTAACCTGAAACTTATCGTTGTATTTTTGGTAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGAt < 2:504025/99‑1 (MQ=255) cGCTAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATg < 1:1590569/100‑1 (MQ=255) tAACCTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCg < 2:1698101/100‑1 (MQ=255) cTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTc < 1:1435680/99‑1 (MQ=255) cTGAAACTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCg < 1:212247/100‑1 (MQ=255) cTTATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGAATCAGGCGGTTGAACGGGAGAAACAGATGCCGTTCGATTTGa > 1:261456/1‑100 (MQ=255) ttATCGGCGCATTACTGGAAGATAGGGTGCAACATGTGGAACTTTGCGATAGCGGGTATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGAt < 1:377717/100‑1 (MQ=255) tATCGGCGCATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATc < 2:1616024/100‑1 (MQ=255) gcgcATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGTGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAAt > 2:1331475/1‑100 (MQ=255) gcgcATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTTAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAAt < 1:1399432/100‑1 (MQ=255) gcgcATTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTCGATCTTaag > 2:1721171/1‑99 (MQ=255) cATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGACGATTGAACAGGCGAAACAGATGACGTTCGATTTGATCTTAATGGa > 2:491979/1‑100 (MQ=255) aTTACTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGat > 2:991232/1‑100 (MQ=255) | ACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGAT > NC_000913/2917020‑2917210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |