| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,008,075 | A→G | D189G (GAC→GGC) | ygeX → | 2,3‑diaminopropionate ammonia lyase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,008,075 | 0 | A | G | 98.0% | 170.1 / ‑4.1 | 53 | D189G (GAC→GGC) | ygeX | 2,3‑diaminopropionate ammonia lyase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (29/21); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01 | |||||||||||
GCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATG > NC_000913/3007984‑3008169 | gCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAACAGCACGGCTGGGAAGTGGTACAGGGCACGGCAt > 2:1708/1‑100 (MQ=255) ccGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATg < 1:223271/100‑1 (MQ=255) cGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATgg > 1:1505131/1‑100 (MQ=255) gTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGaa < 1:455603/100‑1 (MQ=255) gATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATAGGAAGGTTACACCaaaa > 2:992916/1‑100 (MQ=255) tatGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATc > 1:111093/1‑100 (MQ=255) atGAACTATTACGATACTGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc < 2:761032/100‑1 (MQ=255) tGAACTATGACGATACCGTTAGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATccc > 1:1111319/1‑100 (MQ=255) gAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCa < 1:1429534/100‑1 (MQ=255) aCTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCaa > 2:1174906/1‑99 (MQ=255) cTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAAACCCAAcc > 2:875841/1‑100 (MQ=255) tATGACGATACCGTTCGCCTTACCATGCAACTCGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGGACGGCATGGGAAGGTTACACCAAAATCCCAAc < 2:99192/98‑1 (MQ=255) tATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCt > 1:1389332/1‑100 (MQ=255) tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTgg > 2:384602/1‑100 (MQ=255) tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGCCATGGGAAGGTTACACCAAAATCCCAACCTgg > 1:578941/1‑100 (MQ=255) tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTgg < 2:1152080/100‑1 (MQ=255) gACAATAACGTTCGACTGACCATGCAACACGCGCAGCAGCACGGCAGGACAGAGGTAAAGGCCGCGGCATGGGAAGGTTACACCAAAATCCCAAc > 1:1081848/1‑95 (MQ=255) cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc < 2:1603201/100‑1 (MQ=255) cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc > 2:1530787/1‑100 (MQ=255) gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa < 1:1463563/100‑1 (MQ=255) ccGTTCGCCTGACCATGCAACACGCGCAGCAGCCCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAATATAACACAAAAATCCAAACATGGATCATTCa > 2:573645/1‑100 (MQ=255) ccGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCa > 2:893601/1‑100 (MQ=255) cTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACg < 2:1389342/100‑1 (MQ=255) tGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGc < 1:424465/100‑1 (MQ=255) tGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGc < 2:1137516/100‑1 (MQ=255) aTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc < 1:64782/100‑1 (MQ=255) tGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCt > 2:1474824/1‑100 (MQ=255) cacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAg > 2:34012/1‑100 (MQ=255) gcgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATg < 1:1104295/100‑1 (MQ=255) cagctgcaCGGATGGGAAGTGGTACAGGTCACGGTATGGGAAGGTTACACCAAAATCTCAACCTTGTTCATGCAAGGCTACGCAACCCTGGCAGATGAAg < 1:385466/100‑1 (MQ=255) gcagcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGcc > 2:112544/1‑100 (MQ=255) agcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGt < 1:1621521/100‑1 (MQ=255) gcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCg > 2:225471/1‑98 (MQ=255) gcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTc < 2:651977/100‑1 (MQ=255) cGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg > 1:1185556/1‑100 (MQ=255) ggaggggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCGAACCTGGAGCATGCAAGGCTACGCAACCCTGGCAGATGAATCCGTCGa < 1:77393/94‑1 (MQ=255) ggCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGc < 1:1737326/100‑1 (MQ=255) ggCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGc > 1:1816858/1‑100 (MQ=255) gCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa > 2:290428/1‑100 (MQ=255) gCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa < 1:1010031/100‑1 (MQ=255) gggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaaa > 2:1586943/1‑99 (MQ=255) gggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAAt > 2:1439099/1‑100 (MQ=255) gggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAAt < 1:1708/100‑1 (MQ=255) gggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAAt > 2:935088/1‑100 (MQ=255) gAAGTGGTACAGGGCACGGCATAGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATg > 1:719849/1‑99 (MQ=255) aaGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCg > 1:502163/1‑100 (MQ=255) gTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGTAAATGCGTg < 1:1505721/100‑1 (MQ=255) gTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTg < 2:73370/100‑1 (MQ=255) ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa > 1:1368938/1‑100 (MQ=255) ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa > 1:1359715/1‑100 (MQ=255) tACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGAGAAAt > 2:352575/1‑100 (MQ=255) aCAGGGCACGGCATGGGAAGGTTAAACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATg < 1:1711086/100‑1 (MQ=255) aGGGCACGGCATGGGAAGGTTAAACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa > 1:6739/1‑95 (MQ=255) | GCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATG > NC_000913/3007984‑3008169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |