| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 349,708 | C→T | intergenic (+136/‑304) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 349,708 | 0 | C | T | 86.8% | 65.2 / 8.8 | 54 | intergenic (+136/‑304) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base T (15/31); total (21/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCT > NC_000913/349612‑349805 | gCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGAATGGCGACGCATACGACAACCGATGCCTGATGcgtcgc > 2:1515179/1‑100 (MQ=14) gACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGa < 2:1021703/100‑1 (MQ=14) aCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGAc < 2:841814/100‑1 (MQ=14) aCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGAc < 2:1647024/100‑1 (MQ=14) aCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGAAAGCCGATAACTGATGCGTCGCTGACgcca > 1:1647181/1‑96 (MQ=14) cgcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGCTGCCTGATGCCTCGCTGACgccact > 2:796470/1‑95 (MQ=11) cgcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACt > 2:1012326/1‑100 (MQ=255) cgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTa < 1:1442956/100‑1 (MQ=11) gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTAt < 2:1411585/100‑1 (MQ=14) ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAg < 1:964608/100‑1 (MQ=11) tATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAgg < 2:1293722/100‑1 (MQ=14) tATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAgg > 2:1382304/1‑100 (MQ=255) tATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAgg > 1:367751/1‑100 (MQ=255) aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGc < 2:1597830/100‑1 (MQ=14) aTCAGCCCTAGGAGGTGCACCAAACTTTACGTCTGATAATACGGATGGCATCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCTACTTATCAgg < 1:144873/99‑1 (MQ=2) tCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGcc < 2:946263/100‑1 (MQ=11) tCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCAGCTTATCAg > 1:1498545/1‑97 (MQ=14) cAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCt > 1:118131/1‑100 (MQ=255) cAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCt > 1:564036/1‑100 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTa > 1:1353040/1‑100 (MQ=14) ggCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTAc > 2:1535483/1‑100 (MQ=255) ggCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTAc > 2:1809378/1‑100 (MQ=14) cTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACgg < 2:901109/99‑1 (MQ=11) cTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg > 2:1749451/1‑99 (MQ=255) tACTAGGTTCACTGAACTTTAGTTCGGATAATACGTATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACgg < 2:785067/98‑1 (MQ=11) tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACgggg < 2:209550/100‑1 (MQ=11) tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACgggg < 2:701500/100‑1 (MQ=11) tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACgggg < 1:427088/100‑1 (MQ=11) cGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTg < 1:218130/100‑1 (MQ=255) cGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTg < 2:1539576/100‑1 (MQ=11) gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGc < 2:935050/100‑1 (MQ=11) gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGc < 2:1353050/100‑1 (MQ=11) gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGc < 1:1012316/100‑1 (MQ=11) aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCa < 1:179621/100‑1 (MQ=11) ggTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCAc < 1:1594639/100‑1 (MQ=11) ccgatcTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACt < 2:989029/95‑1 (MQ=255) gCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGa > 1:373194/1‑100 (MQ=255) aCTGAACTGTATGTCGGTTAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAc < 1:624695/100‑1 (MQ=11) aCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAc > 1:487018/1‑100 (MQ=255) aCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAc < 2:1410113/100‑1 (MQ=11) aCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAc < 2:962958/100‑1 (MQ=11) aCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAc < 2:1365498/100‑1 (MQ=11) gAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGt > 1:1297937/1‑100 (MQ=11) gAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACtagatcgg > 1:989021/1‑93 (MQ=255) ggTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATTCGGCGGTGACGCGACTTATCAAACCTACGGGGAGCACTGAACTgtcggtcg > 1:166085/1‑98 (MQ=11) ggTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACAGGGTGCACTAAACAGTAAGTCGGa > 2:1652519/1‑100 (MQ=14) cGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa < 1:1071019/100‑1 (MQ=11) gATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAGTGTAGGTCGGaagaga > 1:1062353/1‑95 (MQ=11) gATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa < 2:1694419/100‑1 (MQ=11) aGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGGACTGTAGGTCGGATAAGACGCa > 2:1566399/1‑100 (MQ=11) aGACGGATGGCGACGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGATGCACTGAACTGTAGGTCGGATAAGACGCa > 2:1191873/1‑100 (MQ=11) gACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCAt < 1:35519/100‑1 (MQ=11) cGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAg < 2:1352740/100‑1 (MQ=11) cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc < 2:1683534/100‑1 (MQ=32) gttgtcTGATGCGTCGCTGACGTGACTCACCAGGCCTACGGGGTGCTCGGAACTGTAGGTCGCATAATTTGCATTGTTTCGCATTCGACAAGCGaaac < 1:860384/93‑4 (MQ=2) cgccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCt < 2:564040/100‑1 (MQ=33) | GCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCT > NC_000913/349612‑349805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |