Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,542,093 | G→A | V563M (GTG→ATG) | feoB → | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,542,093 | 0 | G | A | 98.4% | 207.0 / ‑4.1 | 63 | V563M (GTG→ATG) | feoB | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (26/36); total (27/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 |
CAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGC > NC_000913/3541996‑3542187 | cAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTg > 1:153321/1‑100 (MQ=255) gCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCa < 1:430887/100‑1 (MQ=255) gCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCa < 1:1666824/100‑1 (MQ=255) gCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGCCGCTGGCCTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGACATGCa > 2:325977/1‑100 (MQ=255) ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCTCCCCGTTCTTCAAGCCAATTGGcatgcatg < 1:1082398/100‑1 (MQ=255) ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGcatgcatg < 2:1180406/100‑1 (MQ=255) ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGcatgcatg < 2:1513393/100‑1 (MQ=255) tctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGa > 2:1308087/1‑100 (MQ=255) tctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGa < 1:1291755/100‑1 (MQ=255) cGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGa < 1:641149/100‑1 (MQ=255) cGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGa < 1:803165/100‑1 (MQ=255) gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCTATTGGCATGCATGAAGATAACt < 1:1442712/99‑1 (MQ=255) gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTg < 2:181653/100‑1 (MQ=255) gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTg < 2:179514/100‑1 (MQ=255) gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTagc < 2:1625768/100‑1 (MQ=255) gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcag > 1:1108541/1‑100 (MQ=255) gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcag > 1:1036524/1‑100 (MQ=255) aaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGGCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGTATGAAGATAACTggcaggc < 1:1164076/100‑1 (MQ=255) aaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACaggcaggc > 2:1759003/1‑100 (MQ=255) aaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcaggca > 2:1438834/1‑100 (MQ=255) gATAACATCAACGGCTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTg < 2:585723/100‑1 (MQ=255) gATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTg < 2:1271877/100‑1 (MQ=255) aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGTTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTgg < 1:67264/100‑1 (MQ=255) aaCATCAACGTCTCGGCGCTGGCGCCCGTCAGTCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGcc < 1:903971/100‑1 (MQ=255) cATCAACGACTCGGCGCTGGCGTCCGTCAGCTGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg < 2:991462/100‑1 (MQ=255) ncAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGtt > 1:281284/2‑100 (MQ=255) cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATTACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGTTTGGCCTGttt > 1:1152042/1‑100 (MQ=255) cTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAgg < 1:1061223/99‑1 (MQ=255) cGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc > 2:1550470/1‑100 (MQ=255) cGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc > 2:1368726/1‑100 (MQ=255) ggCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc > 2:1136664/1‑100 (MQ=255) tGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc < 2:291692/100‑1 (MQ=255) tGGCGTCCGCCAGCCGGGTGATCACTCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc < 2:1141863/100‑1 (MQ=255) ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg > 1:263049/1‑100 (MQ=255) cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa > 2:1205757/1‑100 (MQ=255) cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGAATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa < 2:130022/100‑1 (MQ=255) tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaag < 1:846624/100‑1 (MQ=255) ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga > 1:1229043/1‑100 (MQ=255) cgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa > 2:1417750/1‑100 (MQ=255) gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt < 1:1683655/100‑1 (MQ=255) ccGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTa > 2:135654/1‑100 (MQ=255) ccGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTa > 2:761682/1‑100 (MQ=255) cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg < 2:1351284/100‑1 (MQ=255) cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg > 1:1798182/1‑100 (MQ=255) ggttgtCACCCCGGTCTTCAAGCCAATTGGCAGGTATGAAGATAAGTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCACGGCGAAAGAAGTGGTAGTg < 2:877517/95‑1 (MQ=255) ggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg < 1:529993/100‑1 (MQ=255) ggTGATCACCCCGGTCTTCAAGCCAACTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg < 1:759976/100‑1 (MQ=255) gTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg < 1:325975/99‑1 (MQ=255) tGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTggg < 2:300988/100‑1 (MQ=255) gATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGt > 1:539663/1‑100 (MQ=255) aTCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa < 2:1817740/100‑1 (MQ=255) ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTaag > 1:1810178/1‑96 (MQ=255) ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGc > 1:742991/1‑99 (MQ=255) cccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTc > 1:1173789/1‑100 (MQ=255) cccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTc > 1:259019/1‑100 (MQ=255) cGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa < 1:1136653/100‑1 (MQ=255) tCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacac < 2:1609764/100‑1 (MQ=255) ttCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc < 2:263050/100‑1 (MQ=255) ttCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc < 1:462523/100‑1 (MQ=255) tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct < 1:1661779/100‑1 (MQ=255) aaGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct < 1:1401897/100‑1 (MQ=255) aGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa > 2:1215751/1‑100 (MQ=255) cAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac > 1:39231/1‑100 (MQ=255) cAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac > 1:1734360/1‑100 (MQ=255) aTTGGCATGTATGAAGATGACTGGCAGGTAACGGTTGGACTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg < 2:1204527/100‑1 (MQ=255) ttGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGc < 1:90519/100‑1 (MQ=255) | CAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGC > NC_000913/3541996‑3542187 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |