Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,886 A→G Y364H (TAT→CAT)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,8860AG95.7% 228.3 / ‑2.0 70Y364H (TAT→CAT) cpxAsensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (35/32);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATAACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACTGTCAA  >  NC_000913/4103789‑4103982
                                                                                                 |                                                                                                
cgATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGAc                                                                                                <  1:1478454/100‑1 (MQ=255)
    cTTCCGGTCTAACGCCAGGACCATAGACGTCCACCGTAATGGAAATACCGTCTTTATCCACGGCAAAGCGCACTGCAATCTTCGCATGGGAATAACGCag                                                                                            >  1:626982/1‑100 (MQ=255)
    cTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCag                                                                                            >  1:643297/1‑100 (MQ=255)
    cTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCag                                                                                            <  2:1315602/100‑1 (MQ=255)
     ttCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCag                                                                                            <  2:1315459/99‑1 (MQ=255)
           gCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTa                                                                                     <  2:1213728/100‑1 (MQ=255)
           gCTAACGCCAGGACCATCGTCGGCCACCGTAATGGTGATACCGTGTTTATCTACCGCAACGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTa                                                                                     <  1:606265/100‑1 (MQ=255)
              aaCGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTAc                                                                                    >  2:427101/1‑98 (MQ=255)
              aaCGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGa                                                                                  >  2:718900/1‑100 (MQ=255)
               aCGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGaa                                                                                 <  2:867573/100‑1 (MQ=255)
                  ccAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACaa                                                                              >  1:1751090/1‑100 (MQ=255)
                   cAGGACCATCTTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAAt                                                                             <  2:1398171/100‑1 (MQ=255)
                    aGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATg                                                                            <  1:192530/100‑1 (MQ=255)
                    aGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATg                                                                            >  1:203126/1‑100 (MQ=255)
                     ggACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGt                                                                           <  2:1131648/100‑1 (MQ=255)
                      gACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGtt                                                                          >  2:726551/1‑100 (MQ=255)
                        ccATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGtttt                                                                        >  1:65675/1‑100 (MQ=255)
                          aTCGTCGTCCACCGTAATGGTGATACTGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTcc                                                                      <  2:1471653/100‑1 (MQ=255)
                          aTCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATAACGCAGAGCATTACGAACAATGTTTTcc                                                                      >  2:1362688/1‑100 (MQ=255)
                           tcgtcgTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCa                                                                     >  1:516240/1‑100 (MQ=255)
                            cgtcgtCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAg                                                                    <  1:1118110/100‑1 (MQ=255)
                              tcgtcCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCTCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAgcg                                                                  <  2:484373/100‑1 (MQ=255)
                              tcgtcCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAgcg                                                                  <  1:1762967/100‑1 (MQ=255)
                                 tcCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGTATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCAc                                                               <  1:1121831/100‑1 (MQ=255)
                                 tcCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCAc                                                               >  2:1697201/1‑100 (MQ=255)
                                 tcCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCAc                                                               <  1:715035/100‑1 (MQ=255)
                                  ccACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGACCAATGTTTTCCAGCGCACt                                                              >  1:153929/1‑100 (MQ=255)
                                   cACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAACATTACGAACAATGTTTTCCAGCGCACtt                                                             >  1:286064/1‑100 (MQ=255)
                                    aCCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACttt                                                            >  1:247448/1‑100 (MQ=255)
                                    aCCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACttt                                                            <  1:1745057/100‑1 (MQ=255)
                                    aCCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACttt                                                            >  2:1434264/1‑100 (MQ=255)
                                     ccGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTc                                                           >  1:209006/1‑100 (MQ=255)
                                     ccGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTc                                                           >  2:617210/1‑100 (MQ=255)
                                       gTAATGGTGATACCGTTTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTcc                                                          <  2:121419/99‑1 (MQ=255)
                                       gTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCa                                                         <  1:1248569/100‑1 (MQ=255)
                                          aTGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAggg                                                      <  2:1772647/100‑1 (MQ=255)
                                           tGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGc                                                     <  1:109198/100‑1 (MQ=255)
                                             gTGATACCGTCTTTATCTACCTCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGctt                                                   >  1:723865/1‑98 (MQ=255)
                                              tGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGtt                                                  >  2:588737/1‑100 (MQ=255)
                                                  aCCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCg                                                >  2:1004207/1‑98 (MQ=255)
                                                  aCCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGa                                              >  1:1649832/1‑100 (MQ=255)
                                                          tATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTa                                      >  1:848625/1‑100 (MQ=255)
                                                           aTCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTAc                                     >  2:186758/1‑100 (MQ=255)
                                                                ccGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCgg                                <  1:1697188/100‑1 (MQ=255)
                                                                ccGCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCgg                                >  2:1292138/1‑100 (MQ=255)
                                                                  gCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGcc                              <  2:848631/100‑1 (MQ=255)
                                                                  gCAAAGCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGcc                              >  2:1472646/1‑100 (MQ=255)
                                                                       gCCCACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGc                         <  2:203126/100‑1 (MQ=255)
                                                                        cccACTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGcc                        <  1:617206/100‑1 (MQ=255)
                                                                           aCTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAg                     >  1:434192/1‑100 (MQ=255)
                                                                            cTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTAGGATTGCCGTACAACGGCAACGGCCCAgg                    >  2:580901/1‑100 (MQ=255)
                                                                            cTTCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGCGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAgg                    >  2:1123043/1‑100 (MQ=255)
                                                                             ttCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGccc                       <  1:1761474/96‑1 (MQ=255)
                                                                             ttCAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGc                   <  2:643301/100‑1 (MQ=255)
                                                                               cAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCgg                 >  2:1377472/1‑100 (MQ=255)
                                                                               cAATCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCgg                 >  1:196187/1‑100 (MQ=255)
                                                                                aaTCTTCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGTACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCggg                <  2:482597/100‑1 (MQ=255)
                                                                                   cTTCGTATGGGAATAACGCAGGGCATTACGAACAATGTTTTCCAGCGCACTCTCCAGGGCGTTCGGGTTGCCGTACAGCGGCCACGGCCCAGGCGGGAAg             <  2:672789/100‑1 (MQ=255)
                                                                                    ttCGTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGt            >  1:1172647/1‑100 (MQ=255)
                                                                                     ttgtaggcgAATGACGCAGAGTATTACGAAAAATGTCTTCCAGCGCACTTTCCAGGGCGTTCGGATTGACGTACAGTGGCCACGGCCCAGGCGGGAAGtt           <  1:1272669/92‑1 (MQ=255)
                                                                                      cGTATGGGAATGACGCAGAGCATTACGATCAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTa          <  2:65675/100‑1 (MQ=255)
                                                                                       gTATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTaa         >  1:217395/1‑100 (MQ=255)
                                                                                        tATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAAc        >  1:1483557/1‑100 (MQ=255)
                                                                                        tATGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAAc        <  1:588733/100‑1 (MQ=255)
                                                                                         aTGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACt       <  2:516244/100‑1 (MQ=255)
                                                                                          tGGGAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACTg      >  1:1152945/1‑100 (MQ=255)
                                                                                            ggAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACTGTc    >  2:1122721/1‑100 (MQ=255)
                                                                                            ggAATGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAAATGTc    >  2:1295159/1‑100 (MQ=255)
                                                                                              aaTGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACTGTCaa  <  1:1734813/100‑1 (MQ=255)
                                                                                              aaTGACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCAAGGACGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACTGTCaa  >  1:1594373/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCCACTTCAATCTTCGTATGGGAATAACGCAGAGCATTACGAACAATGTTTTCCAGCGCACTTTCCAGGGCGTTCGGATTGCCGTACAGCGGCCACGGCCCAGGCGGGAAGTTAACTGTCAA  >  NC_000913/4103789‑4103982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑