Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 751,737:1 (G)6→7 100% coding (433/2448 nt) ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,7311.G64.4% 192.5 / 125.4 87coding (439/2448 nt)ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (16/15);  new base G (33/23);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTC  >  NC_000913/751632‑751827
                                                                                                    |                                                                                                    
cATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTaga                                                                                                       >  1:552733/1‑100 (MQ=255)
    cAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑gggg                                                                                               <  2:964055/100‑1 (MQ=255)
       gTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑ggggggg                                                                                            <  1:307412/100‑2 (MQ=255)
        ttAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGGTGCTGGCAAACAAGTTGGAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑ggggggga                                                                                           <  1:906395/100‑3 (MQ=255)
         tAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGc                                                                                         <  1:794090/100‑1 (MQ=255)
          aGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGcc                                                                                        <  1:83873/100‑1 (MQ=255)
             gatgctgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCa                                                                                      >  1:1512985/3‑100 (MQ=255)
              ctgctgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAg                                                                                     >  2:1642976/1‑100 (MQ=255)
               ttctgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAg                                                                                     <  1:1308523/97‑1 (MQ=255)
               tgctgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGt                                                                                    >  2:1858358/1‑100 (MQ=255)
                  tgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGttt                                                                                  >  1:18066/1‑99 (MQ=255)
                  tgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTc                                                                                <  2:1183383/100‑1 (MQ=255)
                  tgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTc                                                                                <  1:2160643/100‑1 (MQ=255)
                  tgctgcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTc                                                                                >  2:1635358/1‑100 (MQ=255)
                  tgctgcCGTCCTGAGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGtctt                                                                                 >  2:1384659/1‑100 (MQ=255)
                      gcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAg                                                                            <  2:609281/100‑1 (MQ=255)
                      gcCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAg                                                                            >  1:1052367/1‑100 (MQ=255)
                        cGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTg                                                                          <  1:167145/100‑1 (MQ=255)
                        cGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCAAGTTTTCTGAGTg                                                                          >  2:872707/1‑100 (MQ=255)
                           ccTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGcc                                                                        <  1:1931448/100‑1 (MQ=255)
                           ccTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCa                                                                       >  1:637204/1‑100 (MQ=255)
                            cTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCa                                                                       >  1:1079279/1‑100 (MQ=255)
                               tgGGCGGTAGCTCCTCGCAAACAAGTTGTAATCCATCACGACACCCGCAACACCTTCTTCCCATGTAGA‑‑GGCGCGCACCCCAGCTTTCTGAGCCCCACgc                                                                    >  2:1066872/1‑100 (MQ=255)
                                gggCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACgcc                                                                   <  1:1202248/100‑1 (MQ=255)
                                 ggCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACgcca                                                                  >  1:1167085/1‑100 (MQ=255)
                                    ggTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACgccagcc                                                               >  1:2000008/1‑100 (MQ=255)
                                     gTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACgccagcca                                                              >  1:497381/1‑100 (MQ=255)
                                      tAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGccagccag                                                             >  2:672285/1‑100 (MQ=255)
                                       aGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAgg                                                            <  2:1036708/100‑1 (MQ=255)
                                        gctgctGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCt                                                          <  1:2220273/100‑1 (MQ=255)
                                         ctgctgGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCt                                                          >  2:1745503/1‑100 (MQ=255)
                                          tgctgGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCtt                                                         >  2:951489/1‑100 (MQ=255)
                                            ctgGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGTGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGc                                                       >  1:1573878/1‑100 (MQ=255)
                                            ctgGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGc                                                       <  2:1436055/100‑1 (MQ=255)
                                            ctgGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCg                                                      >  1:308186/1‑100 (MQ=255)
                                             tgGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCg                                                      <  2:2207694/100‑1 (MQ=255)
                                              ggCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCgg                                                     <  2:130980/100‑1 (MQ=255)
                                              ggCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGATTGCGGa                                                    >  1:85765/1‑100 (MQ=255)
                                               gCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGa                                                    <  1:910452/100‑1 (MQ=255)
                                                cAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAAt                                                  >  1:485314/1‑100 (MQ=255)
                                                 aaaCAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGa                                                    <  1:307235/98‑1 (MQ=255)
                                                 aaaCAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAAt                                                  <  2:119538/100‑1 (MQ=255)
                                                  aaCAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATa                                                 >  1:396831/1‑100 (MQ=255)
                                                  aaCAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATAc                                                <  1:318074/100‑1 (MQ=255)
                                                   aCAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATAc                                                <  1:1811473/100‑1 (MQ=255)
                                                    cAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACt                                               <  1:1205645/100‑1 (MQ=255)
                                                    cAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACt                                               >  2:592216/1‑100 (MQ=255)
                                                     aaGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTa                                              <  2:806170/100‑1 (MQ=255)
                                                        ttGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAata                                           >  1:727857/1‑100 (MQ=255)
                                                        ttGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAata                                           <  1:1779259/100‑1 (MQ=255)
                                                         tGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAatat                                          >  2:702640/1‑100 (MQ=255)
                                                          gTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATAtt                                         >  2:1084985/1‑100 (MQ=255)
                                                          gTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTc                                        <  1:1190495/100‑1 (MQ=255)
                                                            aaTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCa                                       >  1:708545/1‑100 (MQ=255)
                                                             aTCCATCAGGACACCGGCAACACCTTCTTTTCAGGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATAATAATATTCAg                                      >  2:1476535/1‑100 (MQ=255)
                                                             aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAg                                      >  2:580841/1‑100 (MQ=255)
                                                             aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAg                                      <  2:66328/100‑1 (MQ=255)
                                                             aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGt                                     <  1:1635369/100‑1 (MQ=255)
                                                             aTCCATCAGGACACCGGCAACACCTTCTTTACATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAg                                      >  2:383247/1‑100 (MQ=255)
                                                              tCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGt                                     >  1:511542/1‑100 (MQ=255)
                                                               ccATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGtt                                    >  1:2219272/1‑100 (MQ=255)
                                                                cATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGc                                  >  1:849015/1‑100 (MQ=255)
                                                                cATCACGACACTGGCAACACCTTCTTTCCATGTAGAGGGGGGGCGCCAAGATTTCTGCGTGCCACGCCAGCCAGGATTGCGGAATCCAAATATCCa                                       >  2:1559993/1‑96 (MQ=255)
                                                                  tCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCt                                 <  2:220069/100‑1 (MQ=255)
                                                                  tCAGGACACCGGCAACACCTTCTTTCCAGGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCt                                 <  1:1364563/100‑1 (MQ=255)
                                                                   cAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTg                                <  1:1419424/100‑1 (MQ=255)
                                                                   cAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGa                               >  1:1307476/1‑100 (MQ=255)
                                                                    aGGACACCGGCAACACCTTCTTTCCATGTATAGGGGGGGAGCCCAGTTGTCTTAGTGCCAAGCCAGATAGGCTGGCGGCATACTAATATTAAGTTGCtc                                >  2:1966925/1‑98 (MQ=255)
                                                                    aGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGa                               <  1:2107478/99‑1 (MQ=255)
                                                                         acCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGc                          >  2:2146846/1‑100 (MQ=255)
                                                                              cAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGAt                     >  1:177146/1‑100 (MQ=255)
                                                                              cAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATc                    <  2:1052365/100‑1 (MQ=255)
                                                                               aaCACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATc                    <  1:1669117/100‑1 (MQ=255)
                                                                               aaCACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATc                    <  1:951491/100‑1 (MQ=255)
                                                                                 cacCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGaa                 >  2:1689750/1‑100 (MQ=255)
                                                                                   cattcttTCCATGTAAA‑‑GGAAGAGAGCCGAGTTTTCTGAGTGCCACGAGAGCCAGGCTTGCGGAATTCTAATATGCAGGTGCTGATTTGCTTGATCGaaa                >  2:967329/3‑100 (MQ=255)
                                                                                      tcttTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTg             <  2:2029382/100‑1 (MQ=255)
                                                                                      tcttTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTg             <  2:186330/100‑1 (MQ=255)
                                                                                      tcttTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGa            <  2:836960/100‑1 (MQ=255)
                                                                                       cttTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGa            >  1:490988/1‑100 (MQ=255)
                                                                                        tttCCATGTAGA‑‑GGGAGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGaa           >  2:1031540/1‑100 (MQ=255)
                                                                                        tttCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAag          >  2:533186/1‑100 (MQ=255)
                                                                                         tttcATGTAGA‑‑GGGGGGTAGCACAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAag          <  1:86121/97‑1 (MQ=255)
                                                                                         ttCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAag          >  2:378284/1‑100 (MQ=255)
                                                                                           ccATGTAGAGGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAaga         >  2:1945465/1‑100 (MQ=255)
                                                                                             aTGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCAt     <  2:828481/100‑1 (MQ=255)
                                                                                              tGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCAt     >  2:1036138/1‑100 (MQ=255)
                                                                                              tGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCAt     >  2:903866/1‑100 (MQ=255)
                                                                                              tGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCAt     <  1:725554/100‑1 (MQ=255)
                                                                                               gTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCAttt   >  2:122167/1‑100 (MQ=255)
                                                                                                tAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTc  >  1:1775986/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
CATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTC  >  NC_000913/751632‑751827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: