Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,652,855:1 (G)6→7 100% coding (362/846 nt) sseA → 3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,652,8491.G95.1% 268.4 / 7.5 81coding (356/846 nt)sseA3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base G (42/34);  total (46/35)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGA  >  NC_000913/2652750‑2652948
                                                                                                    |                                                                                                    
aGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCt                                                                                                       >  1:121436/1‑100 (MQ=255)
aGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCt                                                                                                       >  2:646009/1‑100 (MQ=255)
   aTAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTggg                                                                                                   <  2:1669558/100‑1 (MQ=255)
   aTAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCGCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTggg                                                                                                   <  2:1215336/100‑1 (MQ=255)
     aaGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTggggg                                                                                                <  2:165558/100‑1 (MQ=255)
      aGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTgggggg                                                                                               >  1:278769/1‑100 (MQ=255)
       gCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTggggggg                                                                                              <  1:2242364/100‑2 (MQ=255)
          ccTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTgggggggtgg                                                                                           <  2:585395/100‑5 (MQ=255)
           cTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTgggggggtgga                                                                                          <  1:197185/100‑6 (MQ=255)
            tGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGAc                                                                                          >  2:1649007/1‑100 (MQ=255)
            tGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTgggggggtgaac                                                                                         >  1:1204718/1‑94 (MQ=255)
            tGATTGTCTATGACGAAGGTAATATTTTGTCAGAACCACGAGCATGGTGGATGCTGCGCATCTTTGGTGTAGAGAAAGTGTCGATTTTGGGGGGGTGGAc                                                                                          >  2:1332984/1‑100 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTg                                                                                       <  2:795641/99‑1 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                     >  1:1742837/1‑100 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                     >  1:109520/1‑100 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                     >  1:1889034/1‑100 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                     <  1:407851/100‑1 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                     >  1:1348713/1‑100 (MQ=255)
                  tCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAg                                                                                    <  1:396112/100‑1 (MQ=255)
                  tCTATGACGAAGGTAATATTTTCTCAGAAACAAGAGAATGGTGGATGATGAGAACCTTTGGTGTCGCGGCGGTGTAGCTTATGGGGGGTGGAATTGCAgg                                                                                   >  1:1539452/1‑100 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAgg                                                                                   <  1:2221377/100‑1 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                                  >  1:290801/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                                  >  1:1946763/1‑100 (MQ=255)
                      tGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTg                                                                                >  2:1359876/1‑100 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCATGCTg                                                                                >  1:640789/1‑99 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                               <  1:1452317/100‑1 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                               <  2:1584422/100‑1 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGGCGATTCTAGGAGGGAAGACTTGCAGGCTgg                                                                               >  1:1717411/1‑100 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                              <  1:646009/100‑1 (MQ=255)
                          gAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAg                                                                            <  2:473453/100‑1 (MQ=255)
                              gTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGcgcg                                                                        <  2:1946750/100‑1 (MQ=255)
                              gTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGCGGGTGGACTTGCAGGCTGGCAGcgcg                                                                        <  1:1364426/100‑1 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatg                                                                     <  1:1193548/100‑1 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatg                                                                     >  2:2206351/1‑100 (MQ=255)
                                  tCTTTTCTCAGCCCGACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAAAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatga                                                                    >  2:1964404/1‑100 (MQ=255)
                                  tCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatga                                                                    <  2:1809932/100‑1 (MQ=255)
                                   cTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatgat                                                                   >  2:2121196/1‑100 (MQ=255)
                                   cTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatgat                                                                   >  1:598436/1‑100 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                                  <  2:1345136/100‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                                  <  2:91801/100‑1 (MQ=255)
                                       tctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgc                                                               >  1:437598/1‑100 (MQ=255)
                                        ctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgct                                                              >  2:2164449/1‑100 (MQ=255)
                                          cAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGt                                                            >  1:664962/1‑100 (MQ=255)
                                           aGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGtt                                                           <  1:2185493/100‑1 (MQ=255)
                                             ccccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTag                                                         <  2:1598939/100‑1 (MQ=255)
                                              cccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTaga                                                        <  1:2122848/100‑1 (MQ=255)
                                               ccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaa                                                       <  1:860564/100‑1 (MQ=255)
                                                cACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaag                                                      <  2:415019/100‑1 (MQ=255)
                                                cACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaag                                                      >  2:139688/1‑100 (MQ=255)
                                                 aCGAGCATGGTGGATGCTAAGCAACATTGGTATAGAGAAAGTGTAGATTATGGGAGGGTGAACTAGGAGGCTGGAATATAGATGATCTGCAGTTataaga                                                     >  2:762012/1‑100 (MQ=255)
                                                  cGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGaagaag                                                   >  1:1446548/1‑100 (MQ=255)
                                                    aGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                                  <  2:2104102/100‑1 (MQ=255)
                                                     gCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGt                                                 <  2:1141518/100‑1 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                             >  2:737523/1‑100 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                             >  1:2051095/1‑100 (MQ=255)
                                                          gtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTTCAGt                                            >  1:696687/1‑100 (MQ=255)
                                                          gtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGt                                            <  2:1753295/100‑1 (MQ=255)
                                                            ggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTag                                          >  1:821478/1‑100 (MQ=255)
                                                             gATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTaga                                         <  1:574050/100‑1 (MQ=255)
                                                              tatctgcGCACCTTTGGTGTAGGGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                        <  1:1154503/97‑1 (MQ=255)
                                                               tgctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGTAGAAGGTGCAGTAGAGc                                       <  2:89018/100‑1 (MQ=255)
                                                               tgctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGc                                       >  2:595130/1‑100 (MQ=255)
                                                               tgctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGc                                       <  2:2127745/100‑1 (MQ=255)
                                                                gctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCt                                      >  1:1142063/1‑100 (MQ=255)
                                                                 ctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagagaga                                     >  2:1154499/1‑97 (MQ=255)
                                                                  tgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGc                                    >  1:1495520/1‑100 (MQ=255)
                                                                  tgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGc                                    <  1:275690/100‑1 (MQ=255)
                                                                      cACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGa                                >  2:1490386/1‑100 (MQ=255)
                                                                      cACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGa                                <  1:1237859/100‑1 (MQ=255)
                                                                      cACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGa                                >  1:327678/1‑100 (MQ=255)
                                                                      cACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGAGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGa                                >  2:1196704/1‑100 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                             <  2:1188286/100‑1 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                             >  1:1815741/1‑100 (MQ=255)
                                                                             ggTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGagag                         >  2:1717828/1‑100 (MQ=255)
                                                                              gtgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGgaga                          >  2:503410/1‑98 (MQ=255)
                                                                              gtgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGt                        >  2:316859/1‑100 (MQ=255)
                                                                                gtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGttt                      <  1:1465171/100‑1 (MQ=255)
                                                                                gtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGttc                      >  2:790932/1‑99 (MQ=255)
                                                                                  agagAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTaa                    >  2:1728563/1‑100 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTaa                    >  2:2182996/1‑99 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAc                   >  1:126771/1‑100 (MQ=255)
                                                                                         gtgtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCcgcg             <  2:1204714/100‑1 (MQ=255)
                                                                                          tgtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGt            <  2:437599/100‑1 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt           >  1:181677/1‑100 (MQ=255)
                                                                                            tCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGttt          <  2:2086699/100‑1 (MQ=255)
                                                                                             cGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTa         >  1:561366/1‑100 (MQ=255)
                                                                                             cGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAATTTAACGCCGCGTTTa         >  1:145592/1‑100 (MQ=255)
                                                                                             cGATTCTGGGGGGGAGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTa         >  1:1214842/1‑100 (MQ=255)
                                                                                              gATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTaa        >  2:1555053/1‑100 (MQ=255)
                                                                                               aTTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAt       <  1:771556/100‑1 (MQ=255)
                                                                                               aTTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAt       <  1:2121210/100‑1 (MQ=255)
                                                                                                   tGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGa  <  2:1972048/100‑1 (MQ=255)
                                                                                                    |                                                                                                    
AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGA  >  NC_000913/2652750‑2652948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: