| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,809,774:1 | (T)7→8 | 100% | coding (158/738 nt) | ygaZ → | putative L‑valine exporter, norvaline resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,809,767 | 1 | . | T | 95.7% | 214.4 / 3.5 | 69 | coding (151/738 nt) | ygaZ | putative L‑valine exporter, norvaline resistance protein |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base T (27/38); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAC > NC_000913/2809669‑2809861 | gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t > 2:923571/1‑100 (MQ=255) naaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑tt‑t‑tt > 2:2036730/2‑100 (MQ=255) cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑tt‑t‑tt > 1:1462180/1‑100 (MQ=255) gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑tt‑t‑tttttc < 2:129820/100‑3 (MQ=255) gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑tt‑t‑tttttc > 1:1550295/1‑98 (MQ=255) gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCt > 1:1192859/1‑100 (MQ=255) gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCt > 1:676641/1‑100 (MQ=255) ttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGc > 2:751255/1‑100 (MQ=255) ttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGc > 2:751234/1‑100 (MQ=255) aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGcat > 2:1942639/1‑100 (MQ=255) ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGcatc > 1:1268547/1‑100 (MQ=255) gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGcatcat > 2:1807816/1‑100 (MQ=255) tGTTATTAGTTATATTCCGGTGGCCTGTGCGTGCGGTCTGAATACTACCCGTCTGGGATTCTCTCCTCTCGAAATC‑GT‑TT‑T‑TTTTTTCCTGCATCAttt < 2:676933/99‑1 (MQ=255) tGTTATTAGTTATATTACGATGGCCTTTGCATTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TTGT‑TTTTCTCCTGCATCATTTa > 2:863731/1‑100 (MQ=255) ccgatctaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTa < 1:846911/94‑1 (MQ=255) gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTAt > 1:284940/1‑100 (MQ=255) gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTAt < 2:1647450/100‑1 (MQ=255) gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCAACCCGTCTGGGATTCTCTCCTCTCGAAAGCGG‑‑TT‑T‑TTTTCTCCTGCATCATTTAt > 2:1567208/1‑100 (MQ=255) attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCa < 1:1938917/100‑1 (MQ=255) attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCa > 1:34971/1‑100 (MQ=255) ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAg < 1:731918/100‑1 (MQ=255) taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATgaa > 1:726580/1‑96 (MQ=255) taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATAAATGATGCAgg < 1:1554647/100‑1 (MQ=255) aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTaagatcgg > 2:846911/1‑93 (MQ=255) aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAgg > 1:1202486/1‑99 (MQ=255) aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGgc > 1:1204099/1‑100 (MQ=255) gTTATATTCCTGTGGTCTTTGCGTTCGTTCTGAATGCTACCCGTCTGTGATTCTCTCCTCTCGAAAGC‑CT‑TT‑T‑TTTTCTCCTGCATAAATGATGCAggag < 2:569623/100‑3 (MQ=255) gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTGTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TT‑TGTTTTCTCCTGCATCATTTATGCAGacg > 2:1328643/1‑100 (MQ=255) gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGgcg > 2:1147249/1‑100 (MQ=255) gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGgc > 2:1322344/1‑99 (MQ=255) ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATAAATGATGCAggaga < 1:984122/100‑4 (MQ=255) tatTCCGGTGTCTTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAg < 1:869256/100‑1 (MQ=255) atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGc > 2:1584682/1‑100 (MQ=255) atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGAAgga > 1:2091388/1‑93 (MQ=255) ttCCGGTGGCCTTTGCTTTCTGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGcc < 1:103549/100‑1 (MQ=255) ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGcc < 2:1608968/100‑1 (MQ=255) tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGTCCCGTCTGGGATTCTCTCCTCTCGAAGGC‑GTTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGcc < 2:1041435/100‑1 (MQ=255) tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGAATTATTTATGAAggaacaaacca > 1:1199087/1‑91 (MQ=255) ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa < 2:1720745/100‑1 (MQ=255) cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATGCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa < 1:1244725/99‑1 (MQ=255) tttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt < 1:1872713/100‑1 (MQ=255) ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCCTTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg > 1:167162/1‑100 (MQ=255) ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAcc < 1:977306/97‑1 (MQ=255) ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg < 1:1811966/100‑1 (MQ=255) ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg > 2:1799189/1‑100 (MQ=255) gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc < 2:124405/100‑1 (MQ=255) gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc < 2:369058/100‑1 (MQ=255) gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc < 2:2136129/100‑1 (MQ=255) tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGAt > 2:1921908/1‑97 (MQ=255) cttaaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg < 1:264017/97‑1 (MQ=255) cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg < 1:1288099/100‑1 (MQ=255) tGAATGCTTCCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg < 1:545909/100‑1 (MQ=255) tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg < 2:1232284/100‑1 (MQ=255) gAATGCGTCCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGTCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc < 2:1480710/100‑1 (MQ=255) gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc < 1:2070279/100‑1 (MQ=255) aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg > 1:1624657/1‑100 (MQ=255) cGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GTTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGcc > 1:228737/1‑100 (MQ=255) ccGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag < 2:1924702/100‑1 (MQ=255) gTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCGTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg < 2:44619/96‑1 (MQ=255) tGGGATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt < 2:293546/100‑1 (MQ=255) ggATTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtg < 1:1942652/100‑1 (MQ=255) aTTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg < 1:768598/100‑1 (MQ=255) aTTCTCTCCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg < 1:1718181/100‑1 (MQ=255) tctctcCTCTCGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGa < 1:1981739/100‑1 (MQ=255) ctctcctctcGAAAGC‑GA‑TT‑T‑TTTTCTCCTGCATCATTTATGCATGCTCGAGCCAGTTCGTCATTACCGCGATGCTGCCAGCCGGGAGTATTTTGTGGAt > 2:893747/1‑100 (MQ=255) tctcctctcGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt < 1:2154327/100‑1 (MQ=255) ctcctctcGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg > 1:824806/1‑100 (MQ=255) ctcctctcGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCAAGCCACTTCGTCATTACCGCGATGCTGGCAGCCGGGAATAGTTTCTGGATtg > 2:1026974/1‑100 (MQ=255) gatctgAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg < 1:1361906/95‑1 (MQ=255) ctctcGAAAGC‑GT‑TT‑T‑TTTTCTCCTTCATCATTTATGCAGGCGCGAGACAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGGAATTTGTGGATttct > 2:1130507/1‑99 (MQ=255) tcGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc < 1:2201755/100‑1 (MQ=255) tcGAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc < 1:142665/100‑1 (MQ=255) gAAAGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgcttagatc > 2:1361900/1‑94 (MQ=255) aaGC‑GT‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAc < 2:2043447/100‑1 (MQ=255) | GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAC > NC_000913/2809669‑2809861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |