BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,379,266:1	(C)_7→8	100%	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	91.6%	294.8 / 22.6	95	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (6/2); new base C (38/49); total (44/51)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAG  >  NC_000913/3379160‑3379355

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTGTGGTTCTCGATGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑Gttt                                                                                                        >  1:1515479/1‑100 (MQ=255)

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑Gttt                                                                                                        >  1:1119866/1‑100 (MQ=255)

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑Gttt                                                                                                        >  1:1955826/1‑100 (MQ=255)

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTGCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑Gttt                                                                                                        >  1:1577766/1‑100 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGGTGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc                                                                                                    >  2:2053618/1‑100 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c                                                                                                     <  1:1360458/99‑1 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc                                                                                                 >  2:1231238/1‑100 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc                                                                                                 <  2:2214706/100‑1 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑c                                                                                                  <  1:2088606/99‑1 (MQ=255)

     agtgtagtaGAAGAGACCGCTAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc                                                                                                 <  2:2060835/96‑1 (MQ=255)

     aGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc                                                                                                 >  2:320518/1‑99 (MQ=255)

       cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cccc‑c                                                                                             <  2:462353/100‑1 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cccc‑ccgcc                                                                                         >  1:1931006/1‑96 (MQ=255)

              aaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAgc                                                                                     >  2:1657886/1‑100 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAgcgc                                                                                   <  2:1182897/100‑1 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCaa                                                                                >  2:1585618/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCaa                                                                                >  2:1444974/1‑100 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAg                                                                          <  1:722006/99‑1 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGt                                                                         <  1:2068089/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGtt                                                                        <  2:1085416/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGtt                                                                        <  2:1084870/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGtt                                                                        <  1:473481/100‑1 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTAt                                                                     >  1:1299205/1‑100 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTTTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATc                                                                    <  2:1193882/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATc                                                                    <  1:1448532/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATc                                                                    <  1:1303686/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATc                                                                    <  1:698800/100‑1 (MQ=255)

                                 gtgcgtcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCa                                                                  <  2:1690625/95‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCa                                                                  >  2:17729/1‑100 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCa                                                                  <  2:416017/100‑1 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCAt                                                                 >  1:932654/1‑100 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCAtt                                                                <  1:2194866/100‑1 (MQ=255)

                                    tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCAttt                                                               >  2:1436020/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTg                                                              <  2:478700/100‑1 (MQ=255)

                                       ngACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGGTTC‑‑CC‑CCCC‑C‑‑TCCAGCGCCAACCCTAGTTTATCCCTTTg                                                              >  2:2079243/2‑98 (MQ=255)

                                       ctgcGCCCGTTGTCGCCAATCGCCGCTGGTGGCTTGCCCACCGCGGTCAATTTTATC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGtt                                                                        <  2:2002188/85‑1 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTgcg                                                            >  1:353807/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTgc                                                             >  1:1705799/1‑99 (MQ=255)

                                       cGACGCACATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCTAACCATAGTTTATCCATTTgcg                                                            <  2:952446/100‑1 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                         >  1:336063/1‑100 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGAGCCAACCATAGTTTATCCATTTGCGccc                                                         >  2:274980/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                         >  1:816019/1‑98 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                       >  2:518206/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCg                                                        >  1:1220093/1‑99 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                     >  2:897579/1‑100 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTCACGTCGGGGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCGTAGTTTGTCCATTTTCCCCCGGGTTTc                                                  >  2:2103574/1‑99 (MQ=255)

                                                     gctaggggCCGATGGTGGATTTCTAACGTCGTTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCAGGGTGTCATCg                                              <  2:736266/94‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                              >  1:1939374/1‑100 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                              <  1:2048740/100‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                            <  2:1540808/99‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           <  2:1930993/100‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           <  2:1119863/100‑1 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                         >  1:549768/1‑100 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CC‑CCCC‑CT‑TCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           >  2:1229089/1‑98 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           <  2:112323/97‑1 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                        <  1:1616897/100‑1 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                      <  2:745806/100‑1 (MQ=255)

                                                                aTGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                   <  2:1702974/100‑1 (MQ=255)

                                                                 tGGTTGATTTCTAACGTCGGTGAATTCTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                  >  2:2156515/1‑100 (MQ=255)

                                                                   tttGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                                <  1:763707/99‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                               <  1:518205/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                              <  2:2096307/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CCGCCCC‑C‑‑GCCAGCGCCAACCATATTCTATCCATTTGCGCCCGGGTTTCATCGTGGAGTGGCGAAAggc                              <  1:1516458/100‑3 (MQ=255)

                                                                      gATTTCTAACGTGGGTGAATTTTCTC‑GTTT‑‑CCACCCCAC‑‑GCCTGCGCCAACCATATTTTATCCATTTGCGCCTGGGGTTCATTGTGAAGTGGCGAAAGct                              <  2:396376/100‑2 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                             >  1:2070155/1‑100 (MQ=255)

                                                                      gATTGCAAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                              <  2:2151516/99‑1 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTTACGTGGCGAAAGCca                             >  1:626186/1‑99 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                            <  1:1011312/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                           >  2:2127414/1‑100 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                          >  1:567883/1‑100 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                          >  1:2205755/1‑100 (MQ=255)

                                                                           gagcgggcGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTAGCCATGTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                           <  1:184818/90‑1 (MQ=255)

                                                                           cTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                        >  1:1939210/1‑100 (MQ=255)

                                                                            tAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                       <  2:832466/100‑1 (MQ=255)

                                                                              aCGTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                     <  1:549078/100‑1 (MQ=255)

                                                                                gTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   >  2:840335/1‑100 (MQ=255)

                                                                                gTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTGTATCCATCTGCCCCCGGGGTTCAACGTGAAGTGGCGAAAGCcacaagagcgga                   >  2:184818/1‑92 (MQ=255)

                                                                                gTCGGTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATACTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   <  1:1080222/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga                <  2:1999554/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga                <  1:1959925/100‑1 (MQ=255)

                                                                                    gTGAATTTTCGC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCACCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag               <  2:1472267/100‑1 (MQ=255)

                                                                                     tGAATTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt              >  1:1421505/1‑100 (MQ=255)

                                                                                        aTTTTCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag           <  1:2159050/100‑1 (MQ=255)

                                                                                         ttttCTC‑GTTTCCCC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag           <  1:1348446/100‑1 (MQ=255)

                                                                                         ttttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt          >  1:2163053/1‑100 (MQ=255)

                                                                                         ttttCTC‑GTTT‑‑CC‑CCCC‑CCAAACAAAGCCAACCATAGTTTATCCATTTGACCCCGGGTTTCATCGTGAAGTAGAGAAAGCCACAAATGCACCtgagt              >  2:253202/1‑97 (MQ=255)

                                                                                          tttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga            >  2:2086671/1‑97 (MQ=255)

                                                                                          tttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg         <  2:644719/100‑1 (MQ=255)

                                                                                          tttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg         <  1:2009750/100‑1 (MQ=255)

                                                                                           ttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt        >  1:973672/1‑100 (MQ=255)

                                                                                           ttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt        <  1:758010/100‑1 (MQ=255)

                                                                                           ttCTC‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt        >  1:1542534/1‑100 (MQ=255)

                                                                                             ctc‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc      >  2:898188/1‑100 (MQ=255)

                                                                                             ctc‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAATGAGTGTAc      >  1:605875/1‑100 (MQ=255)

                                                                                               cttttt‑‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    <  1:1817587/97‑1 (MQ=255)

                                                                                               c‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    <  1:1444984/100‑1 (MQ=255)

                                                                                               c‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    <  1:1853208/100‑1 (MQ=255)

                                                                                               c‑GTTTC‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGGTTATCCATTTGCCCCCGGGTTGCATCGTGAAATGGCGAGAGCCACAAATGCGCCTGAGTGAGTGTACGc    >  2:1510576/1‑100 (MQ=255)

                                                                                               c‑GTTT‑‑CG‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTTGAGGGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    >  2:1109735/1‑99 (MQ=255)

                                                                                                 gTTT‑‑CC‑CCAC‑C‑‑GCCAGCGCCAACCATAGTTTATACATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAg  >  2:759606/1‑100 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CC‑CCCC‑C‑‑GCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAG  >  NC_000913/3379160‑3379355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`