Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503–1979269	1979278	10–776	74 [0]	[0] 74	insB1–insA	insB1, insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	59 (0.800)	44/162	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCG  >  NC_000913/1978403‑1978601                                                                                                    |                                                                                                    cTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATG                                                                                                     <  2:1768445‑M1/100‑1 (MQ=255)      cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                >  1:2159687‑M1/1‑95 (MQ=255)         gTCACTACACGCACATCCAACGGAGTGGGGCTGCGATTTTCAATACTGCGTGATGCAGATCACACAAAACTCTCATTTACTTAACATAAATgtata                                                                                                 <  2:851996/96‑5 (MQ=255)              tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatact                                                                                        >  2:208106‑M1/1‑87 (MQ=255)              tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                         <  1:979711‑M1/99‑13 (MQ=255)                   gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                   <  2:1088847‑M1/100‑19 (MQ=255)                   gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                   <  1:494589‑M1/100‑19 (MQ=255)                   gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                   <  2:404242‑M1/100‑19 (MQ=255)                    cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                  <  2:375802‑M1/100‑20 (MQ=255)                     acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                  <  2:454084‑M1/99‑20 (MQ=255)                       aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtaatacttttgttttg                                                                               >  2:1742851‑M1/1‑78 (MQ=255)                        tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                              >  1:1636054‑M1/1‑77 (MQ=255)                         aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                             <  1:1036906‑M1/100‑25 (MQ=255)                          cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                            >  1:1907740‑M1/1‑75 (MQ=255)                          cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                            >  1:1176751‑M1/1‑75 (MQ=255)                             cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                         >  1:1095658‑M1/1‑72 (MQ=255)                              ggAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtat                                                                        <  2:482610‑M1/100‑30 (MQ=255)                                agaggggctgCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCATTTACTTAACATAAATGtataagtcatacttttgttttgggtgtattt                                                                      <  2:1915514‑M1/97‑32 (MQ=255)                                 ggggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattt                                                                      >  2:496666‑M1/1‑68 (MQ=255)                                   ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaa                                                                   >  2:407214‑M1/1‑66 (MQ=255)                                      gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatct                                                                >  2:1428417‑M1/1‑63 (MQ=255)                                      gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                  >  1:1276736‑M1/1‑63 (MQ=255)                                        tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                              <  1:1837355‑M1/100‑40 (MQ=255)                                        tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                              <  1:893445‑M1/100‑40 (MQ=255)                                         gCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                             <  2:1588906‑M1/100‑41 (MQ=255)                                            aTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                          >  1:1177390‑M1/1‑57 (MQ=255)                                            aTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                          >  1:58988‑M1/1‑57 (MQ=255)                                             ttttCAATAATGCTTGAGGCAGATCACACAAAACACTTATTTTCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                             <  2:990700‑M1/96‑41 (MQ=255)                                                tCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                      <  1:1726287‑M1/100‑48 (MQ=255)                                                     aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                 <  2:655728‑M1/100‑53 (MQ=255)                                                     aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                 <  2:1374554‑M1/100‑53 (MQ=255)                                                      atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                >  2:1387‑M1/1‑47 (MQ=255)                                                      atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                 >  2:1963304‑M1/1‑47 (MQ=255)                                                         cGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttccttttgttaaacagtttttcgct                                              <  2:1303780‑M1/99‑56 (MQ=255)                                                         cGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcta                                             >  1:1121035‑M1/1‑44 (MQ=255)                                                           tGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatgtgttaaaaagtttttcgctacg                                           <  2:1302128‑M1/100‑59 (MQ=255)                                                           tGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                 >  2:567155‑M1/1‑42 (MQ=255)                                                             aTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgct                                         <  1:1424182‑M1/100‑61 (MQ=255)                                                              tGCAGATCACACAAAACACTCAATTTCTTAACATAAATGtatatgtaatacttttgttttgggtgtatttcaatctgttaaaaaattattcgctacact                                         <  2:967197‑M1/99‑61 (MQ=255)                                                              tGCAGATCACACAAAACACTAAATTACTTAACATAAATGtataagtcatacttttgttattggtgtattacaatctgttaaaaaattattcgttacgcta                                        >  2:1563709‑M1/1‑39 (MQ=255)                                                               gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctag                                       <  2:1988414‑M1/100‑63 (MQ=255)                                                                 aGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatatgttaaaaagtttttcgctacgctagca                                     >  2:790222‑M1/1‑36 (MQ=255)                                                                 aGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgcatttcaatctgttaaaaagtttttcgctacgctagca                                     <  1:150012‑M1/100‑65 (MQ=255)                                                                   aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                   <  1:380495‑M1/100‑67 (MQ=255)                                                                     cacacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagca                                 <  2:360731‑M1/100‑69 (MQ=255)                                                                      acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                <  1:1524882‑M1/100‑70 (MQ=255)                                                                      acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgataaaaagtttttcgctacgctggcaagcaa                                >  1:1276113‑M1/1‑31 (MQ=255)                                                                       cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaa                               >  1:566269‑M1/1‑30 (MQ=255)                                                                       cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaa                               >  1:1139340‑M1/1‑30 (MQ=255)                                                                       cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaa                               <  2:1992961‑M1/100‑71 (MQ=255)                                                                        acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                              >  1:1776509‑M1/1‑29 (MQ=255)                                                                          aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                            >  2:717823‑M1/1‑27 (MQ=255)                                                                          aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                            <  2:1342518‑M1/100‑74 (MQ=255)                                                                           aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatg                           >  2:594442‑M1/1‑26 (MQ=255)                                                                           aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatg                           >  2:1930634‑M1/1‑26 (MQ=255)                                                                           aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                             >  2:1082562‑M1/1‑26 (MQ=255)                                                                             acacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaa                         <  1:1428427‑M1/100‑77 (MQ=255)                                                                              cacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                        >  1:1300499‑M1/1‑23 (MQ=255)                                                                              cacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtctttcgctacgctagcaagcaaaaatgaaa                        >  2:1346068‑M1/1‑23 (MQ=255)                                                                                cTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtaaaaaagtttttcgatacgctagcaagcaaaaatgaaac                       <  2:306234‑M1/99‑79 (MQ=255)                                                                                  cAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtctttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagg                    >  2:1245952‑M1/1‑19 (MQ=255)                                                                                  cAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagg                    <  1:1798329‑M1/100‑82 (MQ=255)                                                                                        cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat              >  2:528302‑M1/1‑13 (MQ=255)                                                                                          tAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcg            <  2:2159672‑M1/100‑90 (MQ=255)                                                                                          tAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcg            <  1:1205743‑M1/100‑90 (MQ=255)                                                                                           aaCATAAATGtataagtcatattttttttttgggtgtatttaaatctattaaaaagtttttcgttacgctagcaagcaaaaatgaaacaggaataatcga           <  2:1078549‑M1/100‑91 (MQ=255)                                                                                           aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcga           <  1:2054304‑M1/100‑91 (MQ=255)                                                                                             cATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa         >  1:1489057‑M1/1‑8 (MQ=255)                                                                                             cATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa         >  1:956410‑M1/1‑8 (MQ=255)                                                                                               tAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat        >  1:153316‑M1/1‑6 (MQ=255)                                                                                                 aaTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggg     >  2:1816726‑M1/1‑4 (MQ=255)                                                                                                   tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat   >  2:1139153‑M1/1‑2 (MQ=255)                                                                                                   tGtataagtcatacttttgttttgggtgtatttcaagctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggga    >  1:1579480‑M1/1‑2 (MQ=255)                                                                                                    gtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatg  >  2:1729961‑M1/1‑1 (MQ=255)                                                                                                    |                                                                                                    CTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCG  >  NC_000913/1978403‑1978601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑