Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 98 [0] [0] 96 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)83 (1.120) 52/162 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

ACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGC  >  NC_000913/258588‑258782
                                                                                                |                                                                                                  
cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                 >  2:2066674‑M2/97‑100 (MQ=255)
cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaagataAGTg                                                                                                 >  2:1606275‑M2/97‑100 (MQ=255)
cacaacaggagatagcaatgacgttaccgagtggacacccgaagagaaaaaaaaaaaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                 <  2:2225843‑M2/4‑1 (MQ=255)
 acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                >  2:40377‑M2/96‑100 (MQ=255)
 acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                <  1:1958291‑M2/5‑1 (MQ=255)
       ggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                          <  1:801844‑M2/11‑1 (MQ=255)
          gatagcaatgacggtaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcaataggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                       <  2:398448‑M2/14‑1 (MQ=255)
            tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                     >  1:1113893‑M2/85‑100 (MQ=255)
            tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                     <  1:2091853‑M2/16‑1 (MQ=255)
              gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                   >  1:523913‑M2/83‑100 (MQ=255)
              gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                   <  1:1852782‑M2/18‑1 (MQ=255)
               caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                  >  2:344602‑M2/82‑100 (MQ=255)
                aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                 >  1:1853889‑M2/81‑100 (MQ=255)
                aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                 <  1:2066688‑M2/20‑1 (MQ=255)
                aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                 >  1:1473803‑M2/81‑100 (MQ=255)
                 atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                >  1:1393253‑M2/80‑100 (MQ=255)
                    acgttaccgagtggacaccccaagagcagattgatcaaaaaatttaccgcactaggcccgaatactcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                             >  1:420236‑M2/77‑100 (MQ=255)
                     cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                            >  2:879746‑M2/76‑100 (MQ=255)
                       ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                          <  2:1989511‑M2/27‑1 (MQ=255)
                       ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                          <  2:2098781‑M2/27‑1 (MQ=255)
                       ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatactcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                          <  2:1382506‑M2/27‑1 (MQ=255)
                        taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                         <  2:1957320‑M2/28‑1 (MQ=255)
                        taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                         <  1:1721899‑M2/28‑1 (MQ=255)
                        taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                         >  1:1989525‑M2/73‑100 (MQ=255)
                          ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAATATAATCGATTCTTTTTCGAtt                                                                       >  2:465707‑M2/71‑100 (MQ=255)
                          ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                       >  1:2052960‑M2/71‑100 (MQ=255)
                           cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactagtcccgtatattcgtgaaggcaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                        <  2:1721241‑M2/29‑1 (MQ=255)
                           cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                      >  1:1299199‑M2/70‑100 (MQ=255)
                            gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                     >  2:1021078‑M2/69‑100 (MQ=255)
                             agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                    <  1:130910‑M2/33‑1 (MQ=255)
                                ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                 >  2:120766‑M2/65‑100 (MQ=255)
                                 gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                <  2:1708794‑M2/37‑1 (MQ=255)
                                 gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                <  2:1834361‑M2/37‑1 (MQ=255)
                                 gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                <  2:701223‑M2/37‑1 (MQ=255)
                                   cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                              <  2:1514710‑M2/39‑1 (MQ=255)
                                   cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                              <  2:307046‑M2/39‑1 (MQ=255)
                                     cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  2:697690‑M2/60‑100 (MQ=255)
                                     cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  1:1209493‑M2/60‑100 (MQ=255)
                                     cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            <  1:493120‑M2/41‑1 (MQ=255)
                                      ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            <  1:614921‑M2/41‑1 (MQ=255)
                                      ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                           >  2:1635735‑M2/59‑100 (MQ=255)
                                        gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            <  2:1803354‑M2/41‑1 (MQ=255)
                                        gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                         <  2:2233057‑M2/44‑1 (MQ=255)
                                         aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                        <  1:2137241‑M2/45‑1 (MQ=255)
                                         aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                        <  1:1453105‑M2/45‑1 (MQ=255)
                                             gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                    >  2:289731‑M2/52‑100 (MQ=255)
                                             gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                    >  1:2060736‑M2/52‑100 (MQ=255)
                                                gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                 >  1:610915‑M2/49‑100 (MQ=255)
                                                gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                 >  2:1753113‑M2/49‑100 (MQ=255)
                                                    gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                             <  2:610915‑M2/56‑1 (MQ=255)
                                                     atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                            >  1:941548‑M2/44‑100 (MQ=255)
                                                     atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                            >  1:719739‑M2/44‑100 (MQ=255)
                                                      tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCCACGTGAAAcc                                           >  1:1913374‑M2/43‑100 (MQ=255)
                                                      tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                           >  1:853100‑M2/43‑100 (MQ=255)
                                                       caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                           >  1:1710539‑M2/42‑99 (MQ=255)
                                                       caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:1947298‑M2/42‑100 (MQ=255)
                                                       caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:2194716‑M2/42‑100 (MQ=255)
                                                       caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:2165891‑M2/42‑100 (MQ=255)
                                                       caaaaaatttaccgcactagacccgtatattcgagaaggtaAGTGCAAAGATAATCGATTCTTTTTTGATTATCTGGCTGTATTCGTCAACGTTaaa                                             >  2:250842‑M2/42‑97 (MQ=255)
                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                           >  1:643673‑M2/41‑98 (MQ=255)
                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  2:151182‑M2/41‑100 (MQ=255)
                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:209989‑M2/41‑100 (MQ=255)
                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:1742550‑M2/41‑100 (MQ=255)
                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:687278‑M2/41‑100 (MQ=255)
                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:1202876‑M2/41‑99 (MQ=255)
                                                         aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                        >  2:1089451‑M2/40‑100 (MQ=255)
                                                           aaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                      <  2:1809832‑M2/63‑1 (MQ=255)
                                                           aaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                      <  2:331896‑M2/63‑1 (MQ=255)
                                                             atttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                    <  1:1793765‑M2/65‑1 (MQ=255)
                                                                  ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                               >  1:2235974‑M2/31‑100 (MQ=255)
                                                                  ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                               >  1:1244887‑M2/31‑100 (MQ=255)
                                                                   cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                              >  2:2153495‑M2/30‑100 (MQ=255)
                                                                   cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                              <  1:763551‑M2/71‑1 (MQ=255)
                                                                    gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                               >  2:1492268‑M2/29‑98 (MQ=255)
                                                                    gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCAGGCTTTATGCGTTAACGTGAAACCGGCACAGGCAATGc                             >  2:32324‑M2/29‑100 (MQ=255)
                                                                    gcactaggcccgtatattcgagaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGAGTCAACCTGGAAACAGCACCGGAAGTGc                             >  2:1086613‑M2/29‑100 (MQ=255)
                                                                     cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                              >  1:62125‑M2/28‑98 (MQ=255)
                                                                      actaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                           <  2:34249‑M2/74‑1 (MQ=255)
                                                                       ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                          <  2:44362‑M2/75‑1 (MQ=255)
                                                                         aggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGaa                        >  1:357439‑M2/24‑100 (MQ=255)
                                                                          ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCAGCACCGGAAGTGCGTGAAt                       >  2:947765‑M2/23‑100 (MQ=255)
                                                                           gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAtt                      >  2:2245427‑M2/22‑100 (MQ=255)
                                                                           gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAtt                      >  1:2154995‑M2/22‑100 (MQ=255)
                                                                            cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                     >  1:1216857‑M2/21‑100 (MQ=255)
                                                                                tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGATTTCTggg                 >  2:1120263‑M2/17‑100 (MQ=255)
                                                                                 atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                <  1:120766‑M2/85‑1 (MQ=255)
                                                                                  tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc               <  2:2052946‑M2/86‑1 (MQ=255)
                                                                                  tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc               <  1:571503‑M2/86‑1 (MQ=255)
                                                                                    ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg             >  1:1960632‑M2/13‑100 (MQ=255)
                                                                                      cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGGAGTGCGTGAATTCTGGGGCtggt           <  1:879221‑M2/90‑1 (MQ=255)
                                                                                       gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg          <  2:219353‑M2/91‑1 (MQ=255)
                                                                                       gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg          <  2:1393246‑M2/91‑1 (MQ=255)
                                                                                          aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat       <  1:344601‑M2/94‑1 (MQ=255)
                                                                                           aggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg      >  1:1029740‑M2/6‑100 (MQ=255)
                                                                                              taAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg   >  2:1455245‑M2/3‑100 (MQ=255)
                                                                                               aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc  <  2:1931931‑M2/99‑1 (MQ=255)
                                                                                                |                                                                                                  
ACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGC  >  NC_000913/258588‑258782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: