| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,243,370 | C→T | 55.5% | L49L (CTG→CTA) | malG ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,243,370 | 0 | C | T | 55.5% | 19.0 / 91.2 | 75 | L49L (CTG→CTA) | malG | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (14/19); new base T (19/22); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
AGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTG > NC_000913/4243272‑4243468 | agTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCa < 2:2142343/100‑1 (MQ=255) gTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAg < 1:856126/100‑1 (MQ=255) aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACTCCAGTTTCCATTGATCCCAGGAGATTTGTTCCGGGATCAgcc < 1:1512976/100‑3 (MQ=255) aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGc < 1:437663/100‑1 (MQ=255) gggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGc < 1:1242928/100‑1 (MQ=255) gggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGc > 1:1978393/1‑100 (MQ=255) ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGcc < 1:1347004/100‑1 (MQ=255) ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGcc < 1:1397795/100‑1 (MQ=255) ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGcc < 1:760907/100‑1 (MQ=255) ggCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCg < 2:1611234/100‑1 (MQ=255) ggCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCg < 1:1907119/100‑1 (MQ=255) gCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGc > 1:664410/1‑100 (MQ=255) cGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCa < 2:677438/100‑1 (MQ=255) gCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGtt < 1:293907/100‑1 (MQ=255) cGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGttt > 2:1955510/1‑100 (MQ=255) gTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTc < 2:145038/100‑1 (MQ=255) tAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCTGGATCAGGCTGCCGGTCGCAAAGTTTcc < 1:847552/100‑1 (MQ=255) aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTccc > 2:895803/1‑100 (MQ=255) aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTccc > 2:685291/1‑100 (MQ=255) aTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCt < 1:1095687/100‑1 (MQ=255) gCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGa < 1:871926/100‑1 (MQ=255) gCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGa > 2:1280476/1‑100 (MQ=255) cGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGAc > 1:2213633/1‑100 (MQ=255) cATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAg > 2:1344748/1‑100 (MQ=255) gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga > 1:162773/1‑100 (MQ=255) gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgag < 2:310449/99‑1 (MQ=255) gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga > 2:493480/1‑100 (MQ=255) cTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATa > 2:1885298/1‑100 (MQ=255) tGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGAt < 2:765230/98‑1 (MQ=255) gtacAACGCTAAAGCCTACCGCCAGTTTCCAGTGATCCCAGGAGATGTGCTCGGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAg < 2:208592/96‑1 (MQ=255) gTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGACTTGCTCCGGGATGAGGCTGCCGGCCGCAACGTTTCCCTGACGCAGCGAGATa > 2:13979/1‑98 (MQ=255) tCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcga > 1:572263/1‑100 (MQ=255) cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGTAGATTTGCTCCGGGATCAGGCTGCCGGTCACAAAGTTTCCCTGACGCAGCGAGATAGcgac < 2:243789/100‑1 (MQ=255) cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac > 2:58565/1‑100 (MQ=255) cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac > 2:535148/1‑100 (MQ=255) gCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGAcc < 2:716829/100‑1 (MQ=255) aaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc > 1:286492/1‑100 (MQ=255) aaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc > 1:195964/1‑100 (MQ=255) aaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc < 2:1350931/100‑1 (MQ=255) agcTAACGCCGGTTTCCAGTGATTCCAGGAGATTTGCTCCAGGATCATGCTGCCTGTCGCAAAGTTTCCCTGACGCAGTGATATAGCGACGACCATcag < 2:852756/97‑1 (MQ=255) ccTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcagca > 1:1258148/1‑100 (MQ=255) ccTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcagca > 1:2241844/1‑100 (MQ=255) cTAACGCCAGTTTCCAGTGATCGAAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCGGCGAGATAGCGACGACCATcagcag < 1:1205253/100‑1 (MQ=255) tAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCagcagc < 2:396538/100‑1 (MQ=255) aaCGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCATCg < 2:1016488/100‑1 (MQ=255) gCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGa > 1:1299057/1‑100 (MQ=255) gCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGa < 2:650275/100‑1 (MQ=255) aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCATCGAGATAGCGACGACCATCAGCAGCGGGAAc < 1:1388241/99‑1 (MQ=255) aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACa > 1:2247018/1‑100 (MQ=255) ccAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATc < 2:96992/100‑1 (MQ=255) naGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCg > 1:723528/2‑100 (MQ=255) cAGTGATCCCAGGAGATTTGCTCCGGGATTCGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCg > 1:1385977/1‑100 (MQ=255) cAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCg > 2:1148407/1‑100 (MQ=255) aGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc > 1:687137/1‑100 (MQ=255) aGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc > 2:1994001/1‑100 (MQ=255) aTCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg > 1:430761/1‑100 (MQ=255) aTCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg < 1:1980510/100‑1 (MQ=255) ttccAGTAGATTTGCTCCGGGATTAGTCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg < 1:92814/97‑1 (MQ=255) cccAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGAt > 1:49272/1‑100 (MQ=255) cccAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGAt < 1:183822/100‑1 (MQ=255) ccAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATa > 1:224772/1‑100 (MQ=255) ggAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa < 2:49272/100‑1 (MQ=255) ggAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa > 1:1606329/1‑100 (MQ=255) gagaTTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaaa < 2:1268232/100‑1 (MQ=255) gaTTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagt < 1:67486/100‑1 (MQ=255) gaTTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagt < 2:28311/100‑1 (MQ=255) gaTTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagt > 2:1422819/1‑100 (MQ=255) tGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagca > 2:1323322/1‑100 (MQ=255) cTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagt > 2:1152939/1‑100 (MQ=255) cTCCGGGATCAGGCTGCCGGTCGCAAAGTTTGCCTGACGCAGCGAGATAGCGACGACAATCAGCAGCGGGAACATAATCGCTGCGAGAAAAag > 2:1894634/1‑93 (MQ=255) cTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagt < 1:1721400/100‑1 (MQ=255) cGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtagc < 2:1960623/100‑1 (MQ=255) cGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtagc < 1:422531/100‑1 (MQ=255) ggATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGtagcagtagcag < 1:895804/100‑1 (MQ=255) tCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTg < 1:501662/100‑1 (MQ=255) | AGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTG > NC_000913/4243272‑4243468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |