Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 715,849 A→G 29.7% L146L (TTA→TTG)  ybfP → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913715,8490AG29.7% 114.3 / 67.6 91L146L (TTA→TTG) ybfPlipoprotein
Reads supporting (aligned to +/- strand):  ref base A (36/28);  new base G (13/14);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTT  >  NC_000913/715750‑715948
                                                                                                   |                                                                                                   
ttGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTa                                                                                                     <  2:1797155/100‑1 (MQ=255)
 tGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTGTTGAGTTac                                                                                                    >  2:170855/1‑100 (MQ=255)
 tGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTac                                                                                                    >  1:507236/1‑100 (MQ=255)
 tGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGACGGGATTATTGAGTTac                                                                                                    >  2:1337810/1‑100 (MQ=255)
  gCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacg                                                                                                   >  1:1601167/1‑100 (MQ=255)
   ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacga                                                                                                  >  2:2110760/1‑100 (MQ=255)
   ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGCGATTATTGAGTTacga                                                                                                  >  2:1312823/1‑100 (MQ=255)
   ccAGGGCAAGCCCAGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacga                                                                                                  >  2:1388344/1‑100 (MQ=255)
    cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacgac                                                                                                 <  2:299763/100‑1 (MQ=255)
     aGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacgacg                                                                                                >  1:2026022/1‑100 (MQ=255)
      gggCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGc                                                                                               >  1:434800/1‑100 (MQ=255)
       ggCAAGCCCGGCACTCGACAGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCt                                                                                              >  2:90183/1‑100 (MQ=255)
        gCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTg                                                                                             >  1:1072693/1‑100 (MQ=255)
         cAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGc                                                                                            >  1:1130895/1‑100 (MQ=255)
          aaGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGcc                                                                                           <  2:470622/100‑1 (MQ=255)
           aGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCa                                                                                          >  1:1942530/1‑100 (MQ=255)
            gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                         <  2:820370/100‑1 (MQ=255)
            gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAg                                                                                         <  1:1920523/100‑1 (MQ=255)
              ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTc                                                                                       <  1:125407/100‑1 (MQ=255)
              ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTc                                                                                       <  2:50071/100‑1 (MQ=255)
              ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTc                                                                                       <  2:1229612/100‑1 (MQ=255)
               cGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCa                                                                                      <  1:808558/100‑1 (MQ=255)
                ggCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAg                                                                                     <  1:345187/100‑1 (MQ=255)
                  cACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGaa                                                                                   >  1:573711/1‑100 (MQ=255)
                      cGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAAtt                                                                               <  1:1388350/100‑1 (MQ=255)
                      cGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGA‑TTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTg                                                                              <  1:164629/100‑1 (MQ=255)
                        aCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGc                                                                             >  2:1203241/1‑100 (MQ=255)
                         ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGcc                                                                            <  2:175797/100‑1 (MQ=255)
                         ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGcc                                                                            <  2:2025335/100‑1 (MQ=255)
                         ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTAGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGcc                                                                            >  2:1243817/1‑100 (MQ=255)
                           gATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTTTTTTTTTTTGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAg                                                                          >  2:1954478/1‑100 (MQ=255)
                               ntCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAg                                                                      >  1:1601404/2‑100 (MQ=255)
                                   aTGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                                                                  <  2:295027/100‑1 (MQ=255)
                                   aTGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                                                                  <  1:1551608/100‑1 (MQ=255)
                                    tGAGGGTTGTGTCAGCGATAGCGGATTTTTTGGCTCGGTGATGCATGGCGGGATTATTGAGTGACGACGCTGCCAGTCAGAAAATGGCCAGCAAGAATTg                                                                 <  2:1043604/100‑1 (MQ=255)
                                    tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                                 <  1:717948/100‑1 (MQ=255)
                                    tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                                 >  1:1074307/1‑100 (MQ=255)
                                       ggATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                                                              >  1:2187015/1‑100 (MQ=255)
                                       ggATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                                                              >  1:186881/1‑100 (MQ=255)
                                         aTTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGc                                                            <  1:1082892/100‑1 (MQ=255)
                                          ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                           <  1:537408/100‑1 (MQ=255)
                                          ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                           <  1:693867/100‑1 (MQ=255)
                                           tATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGa                                                          <  2:195139/100‑1 (MQ=255)
                                            aTGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGAt                                                         <  2:1436968/100‑1 (MQ=255)
                                            aTGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGAt                                                         <  1:480393/100‑1 (MQ=255)
                                            aTGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGAt                                                         <  1:2142409/100‑1 (MQ=255)
                                              gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                       >  1:2198090/1‑100 (MQ=255)
                                              gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                       >  1:1387132/1‑100 (MQ=255)
                                              gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                       >  1:1996863/1‑100 (MQ=255)
                                               tCAGCGAAAGCGGAGTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaaa                                                      >  2:2105809/1‑100 (MQ=255)
                                                cAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAAt                                                     <  1:392388/100‑1 (MQ=255)
                                                 atcGAAAGCGGATTTTTTGTCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAAt                                                     <  2:1417271/97‑1 (MQ=255)
                                                 aGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                    <  2:1389690/100‑1 (MQ=255)
                                                  gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                                   >  1:2182578/1‑100 (MQ=255)
                                                  gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                                   >  1:233722/1‑100 (MQ=255)
                                                   cGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                                  >  2:608180/1‑100 (MQ=255)
                                                    gAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac                                                 >  2:794128/1‑100 (MQ=255)
                                                           gATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaaaa                                          <  2:1477164/100‑1 (MQ=255)
                                                           gATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaaaa                                          >  1:1616418/1‑100 (MQ=255)
                                                           gATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaaaa                                          >  2:70120/1‑100 (MQ=255)
                                                             ttttttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATa                                        >  1:745866/1‑100 (MQ=255)
                                                               ttttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGt                                      <  2:1003719/100‑1 (MQ=255)
                                                                tttGGCTGGATGATGCATGTCGTGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:1974506/100‑1 (MQ=255)
                                                                tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:1012772/100‑1 (MQ=255)
                                                                tttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  1:902909/100‑1 (MQ=255)
                                                                tttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:1072009/100‑1 (MQ=255)
                                                                tttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:1979967/100‑1 (MQ=255)
                                                                  tGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAg                                   >  1:2222593/1‑100 (MQ=255)
                                                                   ggCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGt                                  >  1:1495212/1‑100 (MQ=255)
                                                                     cTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCCGTCAGAAAATTACCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGta                                 >  2:1908366/1‑98 (MQ=255)
                                                                     cTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                                >  1:1138256/1‑100 (MQ=255)
                                                                        gatgatGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCggg                             >  1:1863908/1‑100 (MQ=255)
                                                                          tgatgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGaa                           >  2:505025/1‑100 (MQ=255)
                                                                            atgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATg                         >  2:1109138/1‑100 (MQ=255)
                                                                             tgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                        >  1:1528907/1‑100 (MQ=255)
                                                                             tgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                        >  1:1857008/1‑100 (MQ=255)
                                                                             tgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                        >  1:1462402/1‑100 (MQ=255)
                                                                               cATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGaaa                      >  1:1016883/1‑100 (MQ=255)
                                                                                 tGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                    >  2:353984/1‑100 (MQ=255)
                                                                                   tCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCg                  >  1:1978125/1‑100 (MQ=255)
                                                                                    cGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCgg                 >  1:667915/1‑100 (MQ=255)
                                                                                     gggATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGa                <  2:1942517/100‑1 (MQ=255)
                                                                                      ggATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGa                >  2:863992/1‑99 (MQ=255)
                                                                                      ggATGATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAc                     >  2:1164432/1‑94 (MQ=255)
                                                                                       gATTATTGAGTTACGACGCTGCCAGTCATAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc              <  2:737469/100‑1 (MQ=255)
                                                                                       gATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc              <  2:1635444/100‑1 (MQ=255)
                                                                                         ttattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACACGGACCTg            >  2:1330716/1‑100 (MQ=255)
                                                                                          tattGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGa           >  1:1484113/1‑100 (MQ=255)
                                                                                          tattGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGa           >  2:1508354/1‑100 (MQ=255)
                                                                                          atctGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGa           <  2:602219/97‑1 (MQ=255)
                                                                                           attGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGaa          >  1:1872420/1‑100 (MQ=255)
                                                                                           attGAGTTGCGACGCTGCCAGTCAAAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACATGaa          >  2:1854577/1‑100 (MQ=255)
                                                                                           attGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGaa          <  1:2105823/100‑1 (MQ=255)
                                                                                            ttGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGCAc         >  1:443479/1‑100 (MQ=255)
                                                                                              gAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGaagat       >  1:602219/1‑97 (MQ=255)
                                                                                               aGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATg      <  1:170855/100‑1 (MQ=255)
                                                                                                gTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCAGGGAATGAAACCCAGACCTAAAc         >  2:87495/1‑96 (MQ=255)
                                                                                                  tACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGt     >  2:2175732/1‑98 (MQ=255)
                                                                                                  tACGAAGCTGCTAGTAAGAAAATTGCCAGCAAGAATTGAACGAGTTTAATACACATTAAAATAATGTGTTCTGAAATGATGCCCGGACCTGAAAATGTc    <  2:2077772/99‑1 (MQ=255)
                                                                                                   acgacgCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCtt  <  2:1830806/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTT  >  NC_000913/715750‑715948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑