| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,282,577 | C→T | 24.9% | P905L (CCG→CTG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,282,577 | 0 | C | T | 24.9% | 137.8 / 43.8 | 82 | P905L (CCG→CTG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (30/30); new base T (10/10); total (41/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGG > NC_000913/1282480‑1282672 | ggCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGccctc > 2:1357619/1‑98 (MQ=255)ggCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGc > 2:1971266/1‑100 (MQ=255) gCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt < 2:1108399/100‑1 (MQ=255) gCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt < 2:1805790/100‑1 (MQ=255) gTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATg < 2:2128741/100‑1 (MQ=255) tGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATgg < 1:1752267/100‑1 (MQ=255) gCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATgg < 2:868141/99‑1 (MQ=255) cGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGgag < 1:1641472/100‑1 (MQ=255) cGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGACGACTTACGAACGCTTTACCTCTATCGGCCAGCTGATGgag > 1:1693952/1‑100 (MQ=255) gACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGgaga > 1:980237/1‑100 (MQ=255) gACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGgaga > 1:1576706/1‑100 (MQ=255) gACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGgaga > 1:2108381/1‑100 (MQ=255) aCCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGaa > 1:366896/1‑100 (MQ=255) cTGATCCAAGGTAAAACCGCGAAACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGgag < 1:1371682/96‑1 (MQ=255) tGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGaaaa > 2:2069695/1‑99 (MQ=255) gATCCCAGTTAAAACCGCTACACACATTCTGGTCGTAGAGCGCGCTTATCCGACGACTTACGAACGCTTTACCTCCATCAGCGGGCCGATagaggtaaag < 1:1568433/100‑7 (MQ=16) gATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGaaaa > 1:24690/1‑98 (MQ=255) tCCCAGGTAAACCCGCGCCACACATTATGGTCGGAGAGCGCGATTAGCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:2071863/100‑1 (MQ=255) tCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:329785/100‑1 (MQ=255) cccAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt > 2:2062688/1‑100 (MQ=255) ccAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTa > 2:2062259/1‑100 (MQ=255) ccAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGCAAATCgg > 2:1212186/1‑98 (MQ=255) cAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 2:203130/1‑100 (MQ=255) ggTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg > 1:403767/1‑100 (MQ=255) tAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGc > 1:1725280/1‑100 (MQ=255) aaaCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCgg < 1:1990872/100‑1 (MQ=255) aaCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt < 1:533622/100‑1 (MQ=255) aCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTa < 1:144548/100‑1 (MQ=255) aCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTa > 1:309647/1‑100 (MQ=255) aCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAAGCGGTAATTTCGGTa > 1:1218391/1‑100 (MQ=255) ccGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTa < 2:1595611/99‑1 (MQ=255) cgcCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg < 2:1208720/100‑1 (MQ=255) cgcCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTaaatg < 1:1520460/100‑3 (MQ=255) ccACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGG‑TGTAAAggg > 2:1424071/1‑98 (MQ=255) ccACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCGTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGa < 2:662168/100‑1 (MQ=255) cacaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTg < 1:1200428/100‑1 (MQ=255) cacaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTg > 1:1877857/1‑100 (MQ=255) caTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGAtt > 1:1820575/1‑97 (MQ=255) caTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGcc > 1:1819206/1‑100 (MQ=255) tATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 1:1357625/100‑1 (MQ=255) tATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 2:1818305/100‑1 (MQ=255) aTGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGa < 1:1925403/100‑1 (MQ=255) gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAaca > 1:328209/1‑100 (MQ=255) gTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACAccc < 2:2051885/100‑1 (MQ=255) tAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCa > 2:1397618/1‑100 (MQ=255) gagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCaga > 1:647654/1‑100 (MQ=255) gagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCaga < 2:1100616/100‑1 (MQ=255) gcgcGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGc > 1:2002759/1‑100 (MQ=255) gcgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:1499744/100‑1 (MQ=255) cgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCgag > 2:1394289/1‑100 (MQ=255) cgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCgag > 1:581571/1‑100 (MQ=255) aTTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGCTTGCCTGGAACACCCAGAGCGAAAt > 1:33239/1‑100 (MQ=255) aTTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGAt < 1:1686193/100‑1 (MQ=255) ttATCCGGCGATTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATg < 1:709299/100‑1 (MQ=255) tATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATTGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGAt > 2:903009/1‑98 (MQ=255) ccGGCGACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATc > 1:759930/1‑100 (MQ=255) ccGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATTGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACATCCAGAGCGAGATGGATc < 2:573827/100‑1 (MQ=255) ccGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATc < 2:573757/100‑1 (MQ=255) gACTTACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATctgctg < 1:205031/100‑1 (MQ=255) gACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATctgctg < 1:1481129/100‑1 (MQ=255) aCTTACGAACGCTTTACCTCGATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCtgctgc < 1:990073/100‑1 (MQ=255) tACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTa > 1:1039516/1‑100 (MQ=255) tACGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTa > 2:1544925/1‑100 (MQ=255) tACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTa > 1:1297964/1‑100 (MQ=255) tACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTa < 1:482305/100‑1 (MQ=255) cGAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAg > 1:42501/1‑100 (MQ=255) gAACGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAg < 1:343604/99‑1 (MQ=255) gAACGCTTTACCTCTATCGGCCCGCTTATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGc < 2:1986840/100‑1 (MQ=255) gAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGc < 1:354223/100‑1 (MQ=255) aaCGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCt > 1:630610/1‑100 (MQ=255) aCGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTc > 1:1878288/1‑100 (MQ=255) cGCTTTACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCa < 2:257021/100‑1 (MQ=255) tttACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACt < 1:530176/100‑1 (MQ=255) tACCTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTac > 1:1767752/1‑100 (MQ=255) aCCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCATAGCGAGATGTATCTGCTGCGTAAGCTCAAc < 2:1259740/96‑1 (MQ=255) aCCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTaca < 2:309648/100‑1 (MQ=255) cgatcttCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTacac < 2:528063/94‑1 (MQ=255) ccTCTATCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTacac < 1:1015412/100‑1 (MQ=255) ccTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTacac > 1:2097707/1‑100 (MQ=255) ctctATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACAcc > 1:421384/1‑100 (MQ=255) tCGGCCTGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTacacagatcg > 1:528062/1‑94 (MQ=255) tCGGCCCGCTGATGGAGAAAAACGGAAATGGCGGAAAAGGGATTGCCTGGAACACAAAGAACGAGATGGATCTGCTCCGCAAGCTCAACAACACCaa > 2:729739/1‑97 (MQ=255) ggCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCgg > 1:1456965/1‑100 (MQ=255) | GGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGG > NC_000913/1282480‑1282672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |