Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,489,197	C→T	100%	D322D (GAC→GAT)	aldA →	aldehyde dehydrogenase A, NAD‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,489,197	0	C	T	100.0%	250.7 / ‑6.7	79	D322D (GAC→GAT)	aldA	aldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (40/38);  total (40/39)

TGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGT  >  NC_000913/1489101‑1489293                                                                                                 |                                                                                                 tgtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATAtt                                                                                               >  1:1666368/1‑100 (MQ=255) tgtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATAtt                                                                                               >  1:1203196/1‑100 (MQ=255) ngtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATAtt                                                                                               >  1:1200295/2‑100 (MQ=255)  gtTTATGTACAGAAAGGCAAATATGATCAGTTCGTCAATCGGCTGGTTGAAGCGATGCAAGAGTTTCAATTTCGTAACTCCGCTGAACGCAACGATATTg                                                                                              >  2:796872/1‑100 (MQ=255)    ttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCg                                                                                            >  2:1084960/1‑100 (MQ=255)     tATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCgn                                                                                           <  1:1012091/100‑2 (MQ=255)      aTGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGAt                                                                                          <  1:476134/100‑1 (MQ=255)       tGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTTACCCCGCTGAACGCAACGATATTGCGATg                                                                                         >  2:2197039/1‑100 (MQ=255)       tGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCg                                                                                            >  2:1169635/1‑97 (MQ=255)       tGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGAt                                                                                          >  1:640254/1‑99 (MQ=255)        gTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATgg                                                                                        >  2:2157117/1‑100 (MQ=255)         tACAGAAAGGCATTTATGGTCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATggg                                                                                       >  1:1369386/1‑100 (MQ=255)         tACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATggg                                                                                       <  2:1525282/100‑1 (MQ=255)         tACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATgga                                                                                       >  2:2070893/1‑99 (MQ=255)           caaaaaGGGATTTGTGTTCAGTGCGTCTATCGGGTGGGTTTAGCGATGCTGTCGGTTCAATTTTGTAACCCCGCTGAACGCAACGATATTGCGATgggg                                                                                      <  2:1294977/96‑1 (MQ=255)           cAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGc                                                                                     >  2:48838/1‑100 (MQ=255)             gAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCg                                                                                   >  1:1099518/1‑100 (MQ=255)                aGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTg                                                                                <  1:550151/100‑1 (MQ=255)                 ggCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGa                                                                               >  1:2237132/1‑100 (MQ=255)                   cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt                                                                             >  2:1705289/1‑100 (MQ=255)                   cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt                                                                             <  1:1268104/100‑1 (MQ=255)                   cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt                                                                             >  1:2098281/1‑100 (MQ=255)                   cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGAtt                                                                             <  2:376637/100‑1 (MQ=255)                     tttATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGAGGGGGCCGTTGATTaa                                                                           >  1:841027/1‑100 (MQ=255)                       tattatCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcg                                                                         <  1:17299/100‑1 (MQ=255)                         tGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgac                                                                       >  2:227038/1‑98 (MQ=255)                             cAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcgg                                                                   >  1:1763583/1‑100 (MQ=255)                             cAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTAGATTAACGCCgcgg                                                                   >  2:1864728/1‑100 (MQ=255)                               gTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcggcg                                                                 >  2:709285/1‑100 (MQ=255)                                 tCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcggc                                                                  >  2:1465403/1‑97 (MQ=255)                                    tCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGa                                                            >  2:2106864/1‑100 (MQ=255)                                     cattCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCCCTGGaa                                                           >  2:2105560/4‑100 (MQ=255)                                        tCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAg                                                         <  1:796352/99‑1 (MQ=255)                                          ggCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGt                                                      >  1:1497989/1‑100 (MQ=255)                                          ggCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGt                                                      <  2:1586935/100‑1 (MQ=255)                                          ggCTGGGTGAAGCGATGCAGGCGGTTAAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGt                                                      >  2:321267/1‑100 (MQ=255)                                            cTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCg                                                    >  2:795372/1‑100 (MQ=255)                                             tGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCg                                                    <  1:114309/99‑1 (MQ=255)                                             tGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCg                                                    <  1:114261/99‑1 (MQ=255)                                              gggTGAAGCTATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAg                                                  <  1:579367/100‑1 (MQ=255)                                              gggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAg                                                  <  2:2213699/100‑1 (MQ=255)                                               ggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGc                                                 <  1:2001925/100‑1 (MQ=255)                                               ggTGAAGCGATGCAGGCGCTTCAATTTGGCATCCCCGCTGAACGCAACGATATTGCGATGGGGCCGCTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGc                                                 <  2:1346737/100‑1 (MQ=255)                                                   aaGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCaaaa                                             <  1:1861891/100‑1 (MQ=255)                                                     gCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAg                                           <  2:1929973/100‑1 (MQ=255)                                                      cGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGt                                          <  2:1099516/100‑1 (MQ=255)                                                       gATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTg                                         <  1:45453/100‑1 (MQ=255)                                                       gATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTg                                         <  1:680002/100‑1 (MQ=255)                                                        aTGCAGGCTATTTAATTTGTTAATGGCGATACACGATACGATATTGCGATGGGGCCGTTGATTAACGCCGCTGCGCTGGAAAGGATCGAACAAAAAGTgg                                        <  2:2225976/100‑1 (MQ=255)                                                         tGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGGGCTGGAAAGGGTCGAGCAAAAAGTgg                                        <  1:1366455/99‑1 (MQ=255)                                                           cAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGGCGAGCAAAAAGTGgcg                                      >  1:65810/1‑99 (MQ=255)                                                            aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTACCGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcg                                    <  1:2249495/100‑1 (MQ=255)                                                            aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcg                                    <  1:2153211/100‑1 (MQ=255)                                                            aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgc                                     >  1:1305598/1‑99 (MQ=255)                                                             gtcGGTTCAATTTAGTAACCCCGCTGAACGCGACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcgc                                   >  1:1509458/3‑100 (MQ=255)                                                                ggTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCa                                >  2:1634161/1‑100 (MQ=255)                                                                  ttCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGt                              >  2:277743/1‑100 (MQ=255)                                                                  ttCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGt                              <  1:706352/100‑1 (MQ=255)                                                                  ttCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGt                              <  1:2093415/100‑1 (MQ=255)                                                                   ctaaTTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTa                             <  1:1552280/98‑1 (MQ=255)                                                                    cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGGGCAGTag                            >  1:1748866/1‑100 (MQ=255)                                                                     aaTTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTaga                           >  2:2223091/1‑100 (MQ=255)                                                                     aaTTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTaag                           >  2:1552269/1‑98 (MQ=255)                                                                      agttGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTagaa                          <  1:79795/98‑1 (MQ=255)                                                                        ttGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTagaaga                        <  1:1421071/100‑1 (MQ=255)                                                                           gTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgg                     >  1:1760340/1‑100 (MQ=255)                                                                            tAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAggg                    >  1:1450294/1‑100 (MQ=255)                                                                             aaCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTAGAAAAAAAAAAAAAAAAAGTGGCGCGCGCAGTAGAAGAAgg                     <  2:481310/98‑1 (MQ=255)                                                                               ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg                 <  1:1248518/100‑1 (MQ=255)                                                                                  cGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCgaga              <  1:718745/100‑1 (MQ=255)                                                                                  cGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCgaga              >  1:926535/1‑100 (MQ=255)                                                                                      gAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTgg          <  1:535059/100‑1 (MQ=255)                                                                                       aaCGCAACTATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAAAAAAAAAAAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTgg          <  2:274909/99‑1 (MQ=255)                                                                                       aaCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGc         <  1:637617/100‑1 (MQ=255)                                                                                       aaCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGc         <  2:1450284/100‑1 (MQ=255)                                                                                         cGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGt       <  1:1937444/100‑1 (MQ=255)                                                                                           cAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGt       >  2:2233851/1‑98 (MQ=255)                                                                                             aCGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGACGGGGCGAGAGTGGCGTTCgg   <  2:1362565/100‑1 (MQ=255)                                                                                              cGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGt  >  2:397685/1‑100 (MQ=255)                                                                                                 |                                                                                                 TGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGT  >  NC_000913/1489101‑1489293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑