| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,755,639 | G→A | 100% | intergenic (‑498/‑59) | ydhZ ← / → pykF | uncharacterized protein/pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,755,639 | 0 | G | A | 96.0% | 226.5 / 0.4 | 74 | intergenic (‑498/‑59) | ydhZ/pykF | uncharacterized protein/pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (39/32); total (40/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
GACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA > NC_000913/1755546‑1755735 | gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc > 2:595260/1‑100 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATTTCACCTATCCTGAGAGCGAGGCACCACCACTTTCTTAATACCGGATTCGCTTTCCGACAGTgcgc > 2:1246743/1‑100 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc > 1:1238306/1‑100 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc < 2:1548947/100‑1 (MQ=255) aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa < 2:539711/100‑1 (MQ=255) aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg < 1:171293/100‑1 (MQ=255) cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGaa > 1:1978730/1‑100 (MQ=255) cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg < 1:78913/100‑1 (MQ=255) ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt > 1:1721470/1‑100 (MQ=255) ggtgcaaacaattttggtctttttcatgacagtcttagtctttaagttgagaaggatgggagaaacttGCTTTCCGACAGTGCGCCCAGAAAGCAAGttt > 2:223725‑M1/69‑100 (MQ=255) tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCTCCACTTTCGTAATACCGGATTCGCTTTCCGACAGTTCGCCCAGAAAGCAAGTTtc > 1:100117/1‑100 (MQ=255) tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtc < 2:1977434/100‑1 (MQ=255) gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc < 2:2233617/100‑1 (MQ=255) cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCTCCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc < 2:1083855/100‑1 (MQ=255) aacaattttggtctttttcatgacagtcttagtctttaagttgagaaggatgggagaaacttGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc > 1:406362‑M1/63‑98 (MQ=255) aCGCTTTTTCGATGTCACCTATCCTTATAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc < 2:1959972/98‑1 (MQ=255) caattttggtctttttcatgacagtcttagtctttaagttgagaaggatgggagaaacttGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATc > 1:1347733‑M1/61‑100 (MQ=255) cTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATcc > 2:563913/1‑100 (MQ=255) tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc < 1:1653563/100‑1 (MQ=255) tgtCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc < 2:1359326/98‑1 (MQ=255) tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc < 2:1721459/100‑1 (MQ=255) tCGATGCCACCTATACATAAAGCGAGGCACCACCACTTTCGTAATACCGGATTCCCTTTCCGACAGTGCACCCAGAAAGCAAGTTTCTCCCATCCtt > 2:47404/1‑97 (MQ=255) cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa > 2:1288926/1‑100 (MQ=255) cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCCGATTCGCTTTCCGACAGTGCGCTCAGAAAGCAAGTTTCTCCCATCCTtctt < 2:325390/99‑2 (MQ=255) gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa > 1:488181/1‑100 (MQ=255) aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc < 2:1569290/100‑1 (MQ=255) tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGTATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt < 1:464630/100‑1 (MQ=255) tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt > 2:881434/1‑100 (MQ=255) tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa > 1:2156508/1‑100 (MQ=255) tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa > 1:1092007/1‑100 (MQ=255) tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa > 1:1216109/1‑100 (MQ=255) cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa > 2:2077039/1‑100 (MQ=255) cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCATAAAGCAAGTTTCTCTCATCCTTCTCAACTTaa < 2:385545/100‑1 (MQ=255) cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa > 2:792939/1‑100 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag < 1:1969162/100‑1 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag > 2:1281203/1‑100 (MQ=255) cTATCCTTAGAGCGAGGTACCATCTCTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaaga < 2:2052236/100‑1 (MQ=255) tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac > 2:1963259/1‑100 (MQ=255) tCCTTAGAGCGAGGCACCACCCCTTTCTTAATACCGGATTCCCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagacta < 2:1746812/100‑1 (MQ=255) cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag > 2:1939754/1‑100 (MQ=255) cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag > 1:1180820/1‑100 (MQ=255) ttAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga < 1:792939/100‑1 (MQ=255) gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagact > 1:85408/1‑99 (MQ=255) gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg < 1:2109933/100‑1 (MQ=255) gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt > 2:126530/1‑100 (MQ=255) aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg > 1:2005269/1‑100 (MQ=255) ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa > 1:2190039/1‑100 (MQ=255) accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa < 1:648628/100‑1 (MQ=255) caccacTTTCGTAATACCGGATTCGCTTTCGGAAAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGGCATGAAAAAg > 2:1475501/1‑100 (MQ=255) caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg < 1:414096/100‑1 (MQ=255) caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg > 1:1315328/1‑100 (MQ=255) caccacTTTCGTAATACCGGAATCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATACTTCTCAACTTAAAGACTAAGACTGTCATGaa > 2:1327875/1‑96 (MQ=255) accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa > 2:244426/1‑100 (MQ=255) accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa > 1:1790976/1‑100 (MQ=255) tttCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa < 1:2077053/100‑1 (MQ=255) tCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTAAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAt > 2:1190987/1‑100 (MQ=255) cGTAATTCCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTACCACTGTCATGAAAAAGACCAAAAtt < 2:168628/100‑1 (MQ=255) ntAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg > 1:843663/2‑100 (MQ=255) tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt < 1:1708987/100‑1 (MQ=255) tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg < 2:1525744/100‑1 (MQ=255) aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc > 2:1244208/1‑100 (MQ=255) ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc > 1:1904526/1‑99 (MQ=255) ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa < 1:1674880/100‑1 (MQ=255) ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa < 2:1040169/100‑1 (MQ=255) cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc < 2:1230425/100‑1 (MQ=255) cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc < 1:1356915/100‑1 (MQ=255) ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATTAAAAAGACCAAAATTGTTTGCAcc > 2:305706/1‑100 (MQ=255) ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATAGTTTGCACCATc > 1:1706558/1‑100 (MQ=255) CTTTCCGACCGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACGGTCATAAAAAAGACCAAAATTGTTGGCACCATCGGAc < 2:1514238‑M1/100‑1 (MQ=255) CTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc < 2:994605‑M1/100‑1 (MQ=255) TTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc > 2:1843449‑M1/1‑100 (MQ=255) TTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc > 2:1185914‑M1/1‑100 (MQ=255) TTCCGACAGTGCGCCCAAAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGa < 2:432109‑M1/97‑1 (MQ=255) CCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAACAGACCAAAATTGTTTGCACCATCGGACAGaa < 2:1027353‑M1/100‑1 (MQ=255) | GACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA > NC_000913/1755546‑1755735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |