Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,250 A→G 100% Y448C (TAC→TGC)  yeaG → protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,2500AG97.9% 315.5 / ‑2.7 94Y448C (TAC→TGC) yeaGprotein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (41/50);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

ATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATAT  >  NC_000913/1868153‑1868344
                                                                                                 |                                                                                              
aTGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCg                                                                                              <  1:2175441/100‑1 (MQ=255)
   aaCGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTcc                                                                                           >  2:1561033/1‑100 (MQ=255)
     cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTcccg                                                                                         <  2:708858/100‑3 (MQ=255)
      ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                        >  2:1098940/1‑100 (MQ=255)
      ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                        <  1:1774218/100‑1 (MQ=255)
      ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                        <  1:1470905/100‑1 (MQ=255)
      ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                        >  1:129323/1‑100 (MQ=255)
      ggTCTGTCGACGCGTTTTACGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                        >  2:474063/1‑100 (MQ=255)
       gTCTGTTGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATTTATAAGTGGCAGCAAACCCGTTCCATCTGTTCTGCGTCCTGGa                                                                                       <  2:717749/100‑1 (MQ=255)
       gTCTGTCGACGCGTTTTGCGTTTAAGATGCTCTCCGGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGa                                                                                       >  2:1004222/1‑100 (MQ=255)
       gTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                         >  1:272372/1‑98 (MQ=255)
       gTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGa                                                                                       >  1:1218000/1‑100 (MQ=255)
          tGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAca                                                                                    <  2:178202/100‑1 (MQ=255)
           gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGTGTCCTGGAAcag                                                                                   >  1:1210989/1‑100 (MQ=255)
           gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcag                                                                                   <  1:2209665/100‑1 (MQ=255)
            tCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagc                                                                                  <  1:1189279/100‑1 (MQ=255)
               aCGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGa                                                                               >  1:1263807/1‑100 (MQ=255)
                cgcgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAt                                                                              <  2:607574/100‑1 (MQ=255)
                cgcgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAt                                                                              <  2:86947/100‑1 (MQ=255)
                    ttttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAg                                                                          <  1:1495481/100‑1 (MQ=255)
                     tttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgc                                                                         >  1:1464945/1‑100 (MQ=255)
                         cGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGa                                                                     <  2:1771675/100‑1 (MQ=255)
                          gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATTTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                    <  2:889145/100‑1 (MQ=255)
                          gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGa                                                                     >  2:1229421/1‑99 (MQ=255)
                          gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                    <  1:432403/100‑1 (MQ=255)
                          gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                    <  1:243958/100‑1 (MQ=255)
                           tttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGc                                                                   >  2:2115833/1‑100 (MQ=255)
                            ttAAGATTCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCa                                                                  <  1:994404/100‑1 (MQ=255)
                            ttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCa                                                                  <  2:95248/100‑1 (MQ=255)
                            ttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCa                                                                  <  2:1647205/100‑1 (MQ=255)
                               aGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGtt                                                               >  1:2197834/1‑100 (MQ=255)
                               aGATCCTCGCCCGCGTGTCCAACATCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTTCGCCCTGGAACAGCAGATTGATCGCGAGCAGtt                                                               >  2:668281/1‑100 (MQ=255)
                                gATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTc                                                              >  1:1136065/1‑100 (MQ=255)
                                 aTCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTcc                                                             <  1:183765/100‑1 (MQ=255)
                                 aTCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTcc                                                             <  1:1251486/100‑1 (MQ=255)
                                  tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGttac                                                             >  1:1873365/1‑97 (MQ=255)
                                  tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc                                                            >  1:912240/1‑100 (MQ=255)
                                  tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc                                                            <  1:1049971/100‑1 (MQ=255)
                                  tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc                                                            <  1:634661/100‑1 (MQ=255)
                                  tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAACTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc                                                            <  2:470440/100‑1 (MQ=255)
                                    ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGTAGATTGAGCGCGAGCAGTTCCcac                                                          >  1:2071582/1‑100 (MQ=255)
                                    ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCGGTTcgc                                                            >  2:1522674/1‑96 (MQ=255)
                                    ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcac                                                          >  1:272789/1‑100 (MQ=255)
                                    ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGGCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCca                                                           <  2:2143496/99‑1 (MQ=255)
                                       tcCCGCGTTTTCAACTTCTATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag                                                       <  1:2101634/100‑1 (MQ=255)
                                       tcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag                                                       <  1:1121905/100‑1 (MQ=255)
                                         ccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                     <  1:493126/100‑1 (MQ=255)
                                         ccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                     >  2:1802374/1‑100 (MQ=255)
                                         ccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                     <  2:348582/100‑1 (MQ=255)
                                          cgcgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                     >  2:261619/1‑99 (MQ=255)
                                          cgcgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgc                                                    <  1:941277/100‑1 (MQ=255)
                                              tgtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggc                                                <  1:2006920/100‑1 (MQ=255)
                                              tgtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggc                                                <  2:617416/100‑1 (MQ=255)
                                              tgctCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGCTTGAGCGCGAGCAGTCCCCACAAGAgcaggc                                                <  2:101515/97‑1 (MQ=255)
                                               gtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggca                                               >  2:339155/1‑100 (MQ=255)
                                                 tCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggcag                                              >  1:672124/1‑99 (MQ=255)
                                                 tCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGa                                             >  2:1027070/1‑100 (MQ=255)
                                                   aaCTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAAc                                           >  1:1536433/1‑100 (MQ=255)
                                                    aCTTCGATCATGTAGAAGTGACAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGTCAGAACg                                          >  2:210496/1‑100 (MQ=255)
                                                     cTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGc                                         >  1:1280908/1‑100 (MQ=255)
                                                     cTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCACACAAGAGCAGGCAGAACGc                                         >  2:2135302/1‑100 (MQ=255)
                                                          aTCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCt                                    <  2:1944976/100‑1 (MQ=255)
                                                           tCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTg                                   <  2:247236/100‑1 (MQ=255)
                                                              tGTAGAAGTGGCAGCAAACCAGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                >  2:465351/1‑100 (MQ=255)
                                                                tAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGtt                              <  1:2019644/100‑1 (MQ=255)
                                                                   aaGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCATAAGAGCAGTCAGAACGCTATCTGGAGTTCCt                           <  2:827999/100‑1 (MQ=255)
                                                                    aGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTg                          <  2:1722242/100‑1 (MQ=255)
                                                                    aGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTg                          <  1:92338/100‑1 (MQ=255)
                                                                      tGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaa                        <  2:366845/100‑1 (MQ=255)
                                                                       ggCAGCATACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa                       >  2:409266/1‑100 (MQ=255)
                                                                       ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa                       >  2:1865521/1‑100 (MQ=255)
                                                                       ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa                       >  2:2248570/1‑100 (MQ=255)
                                                                       ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAAAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa                       >  2:804297/1‑100 (MQ=255)
                                                                        gcagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAg                      <  2:1204813/100‑1 (MQ=255)
                                                                         cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                     >  1:1899275/1‑100 (MQ=255)
                                                                         cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                     >  2:2134398/1‑100 (MQ=255)
                                                                         cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                     >  2:1495625/1‑100 (MQ=255)
                                                                          agcaAATCCGGTCCAGCTGTTCGGCGTCCTGGAACAGCAGTTTGGGCGCGAGCAGTTCCTACTAGAGTATGCAGATCGCTATCTGGAGTTCCTGAAAg                      <  2:212344/98‑1 (MQ=255)
                                                                          agcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGt                    <  1:927804/100‑1 (MQ=255)
                                                                            caAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTa                  <  1:330007/100‑1 (MQ=255)
                                                                             aaaCCCGGTCCATTTGTTCTGCGTCCTGGAACAGCAGAGTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGCAAGGTTAt                 >  2:1380032/1‑100 (MQ=255)
                                                                              aaCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATc                >  1:1066195/1‑100 (MQ=255)
                                                                              aaCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATc                <  1:1257438/100‑1 (MQ=255)
                                                                              aaCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATc                <  1:1366683/100‑1 (MQ=255)
                                                                                 ccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGa             <  1:1004224/100‑1 (MQ=255)
                                                                                 ccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGa             <  1:1679302/100‑1 (MQ=255)
                                                                                   ggTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATc           >  1:485131/1‑100 (MQ=255)
                                                                                    gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATcc          <  2:899551/100‑1 (MQ=255)
                                                                                      ccATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCg        >  2:1188123/1‑100 (MQ=255)
                                                                                         tCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaaa     <  1:1894435/100‑1 (MQ=255)
                                                                                         tCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaaa     <  1:402479/100‑1 (MQ=255)
                                                                                          cTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAat    <  1:2078662/100‑1 (MQ=255)
                                                                                            gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAatat  >  2:1148050/1‑100 (MQ=255)
                                                                                            gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAatat  >  2:1550799/1‑100 (MQ=255)
                                                                                                 |                                                                                              
ATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATAT  >  NC_000913/1868153‑1868344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: