| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,674,851 | T→C | 26.5% | H304R (CAT→CGT) | yphC ← | putative Zn‑dependent NAD(P)‑binding oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,674,851 | 0 | T | C | 26.5% | 117.2 / 48.6 | 79 | H304R (CAT→CGT) | yphC | putative Zn‑dependent NAD(P)‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (32/26); new base C (4/17); total (36/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 | |||||||||||
CATAGGCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTA > NC_000913/2674753‑2674948 | cATAGGCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATg > 1:367945/1‑100 (MQ=255) aTAGGCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATgg < 1:223049/100‑1 (MQ=255) aTAGGCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATgg > 2:142246/1‑100 (MQ=255) ggCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAAc > 1:500495/1‑100 (MQ=255) ggCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAAc > 1:1371908/1‑100 (MQ=255) cATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAg > 2:1548405/1‑100 (MQ=255) aTCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGa > 2:557050/1‑100 (MQ=255) tCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGAc > 2:879322/1‑100 (MQ=255) cACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACt > 2:180192/1‑100 (MQ=255) cctgcctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTgg < 1:89899/100‑1 (MQ=255) cctgcctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTgg > 2:1587176/1‑100 (MQ=255) tgcctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTc < 2:2059382/100‑1 (MQ=255) gcctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCa > 2:1206221/1‑100 (MQ=255) gcctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCa > 1:2135905/1‑100 (MQ=255) ctgTTCCAGCGAGAAGCGATGGGTAATGGTGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCAcc < 2:1890422/100‑1 (MQ=255) tgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCAccc < 1:25597/100‑1 (MQ=255) tgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCACATTTTTCCATACGGAACAGACTGGTCAccc < 2:912403/100‑1 (MQ=255) tgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTCCCAGTCCGTCAGACCATGGGCGCATTTTTCCATATGGAACAGACTGGacacgc > 1:977701/1‑98 (MQ=255) gTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCa > 2:395855/1‑100 (MQ=255) cAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCTGAACATGGGCGCATTTTTCGTTATGGAACACACTGGTCACGCAgg > 2:1881975/1‑98 (MQ=255) gCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGcc < 2:615515/100‑1 (MQ=255) aaGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATaa < 2:500496/100‑1 (MQ=255) gCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGACAATc > 1:1893772/1‑100 (MQ=255) cGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATcc > 1:138572/1‑100 (MQ=255) gggTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATccgccg < 1:1266179/100‑1 (MQ=255) gTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGtt > 1:1112875/1‑100 (MQ=255) tGGCGTTACTCGGCCAATGCTTCCCGTCCGTCAGATCATGGACGCTTTTTTCCATACGGAACAGACTGGTCACCCAGTAGCCGATAATCCGCCGTTGa < 2:1437654/98‑1 (MQ=255) ggCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCTTATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGACGTTGATgg > 2:1068836/1‑100 (MQ=255) ggCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATgg < 2:465473/100‑1 (MQ=255) ggCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATgg < 2:2237638/100‑1 (MQ=255) gCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGt > 2:1275571/1‑100 (MQ=255) cGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTg < 2:1800860/100‑1 (MQ=255) cGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTg < 2:1163844/100‑1 (MQ=255) gTTACGGGGCGACAGCTTCCAGTCCGTCAGATCGGGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGc < 1:2124818/100‑1 (MQ=255) gTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGc < 1:1941412/100‑1 (MQ=255) ttACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCa < 1:1420292/100‑1 (MQ=255) ttACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACACAGGAGCCGATAATCCGCCGTTGATGGTGCa < 1:1749784/100‑1 (MQ=255) ttACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCa < 1:1600322/100‑1 (MQ=255) tACGCGGCCACATCTTCCAGTTCGTCATATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATTCGCCGTTGATGGGGCAt < 2:1211013/100‑1 (MQ=255) tACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCAt > 2:1938892/1‑100 (MQ=255) aCGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATc > 1:1996662/1‑100 (MQ=255) aCGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATc < 1:1548416/100‑1 (MQ=255) gcgGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCa < 2:270734/99‑1 (MQ=255) cgGCCACAGCTCCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGa > 2:919842/1‑100 (MQ=255) ggCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGa > 1:896599/1‑99 (MQ=255) gttacaGCTTCCAGTCCGTCAGATCATGTGCGTATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATc < 2:2085455/97‑1 (MQ=255) gCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATc > 1:102608/1‑100 (MQ=255) aGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGgcgc < 1:483926/100‑1 (MQ=255) aGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGgcgc < 1:531357/100‑1 (MQ=255) cTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTg < 1:1592476/100‑1 (MQ=255) tCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGAc < 1:557049/100‑1 (MQ=255) ccAGTCCGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGAcc > 1:397230/1‑100 (MQ=255) cAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCt > 2:445189/1‑100 (MQ=255) gTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCg < 2:1722928/100‑1 (MQ=255) cGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAAt < 1:1844694/100‑1 (MQ=255) cGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAAt < 1:138388/100‑1 (MQ=255) cGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAAt < 2:1797512/100‑1 (MQ=255) cGTCAGATCATGGGCGCATTTTTCCATACGGAACAGACGGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAAt < 1:227583/100‑1 (MQ=255) cGTCAGATCATGGACGCATTTTTTCATATGGTACAGACGGGTCACCCAGGAGCCGATAATACGCCGTTGATGGTGCATCAGATCGGCGCTGCCCTCGAAt > 2:1383228/1‑100 (MQ=255) gTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAAtt < 2:1510245/100‑1 (MQ=255) tCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTc < 2:1531324/100‑1 (MQ=255) cAGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTcc < 1:1225377/100‑1 (MQ=255) aGATCATGGTCTTTTTTTTCCATACGGAACAGACTGGTCACCCAGGAGCAGATAAGCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCa < 1:715696/100‑1 (MQ=255) aGATCATGGGCGCATTTTTCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCa > 2:1071041/1‑100 (MQ=255) ggtCATGGGCGCATTTTTCCATATGGATCAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCa < 2:1191648/97‑1 (MQ=255) gATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCAc > 1:660914/1‑100 (MQ=255) gATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCAc > 1:1531061/1‑100 (MQ=255) gATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCAc > 1:735308/1‑100 (MQ=255) tCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACtt > 1:1033923/1‑100 (MQ=255) ggCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCg > 2:1554611/1‑100 (MQ=255) gcgcATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTcc < 1:2101401/98‑1 (MQ=255) gcgcATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCgg > 1:2097133/1‑100 (MQ=255) gcATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGtt < 2:971527/100‑1 (MQ=255) ttttCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCAc < 2:1889989/100‑1 (MQ=255) ttttCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACCTTTCCGGTTTCAc < 1:1524809/100‑1 (MQ=255) ttCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCa < 1:921323/100‑1 (MQ=255) ttCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCa < 1:391964/100‑1 (MQ=255) ttCCATACGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCa > 1:1849701/1‑100 (MQ=255) cATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATg > 1:1330450/1‑100 (MQ=255) tatGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTa < 1:234972/100‑1 (MQ=255) | CATAGGCATCACCTGCCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTA > NC_000913/2674753‑2674948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |