| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,731,312 | G→A | 100% | intergenic (‑155/+288) | rrsG ← / ← clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,731,312 | 0 | G | A | 97.0% | 98.0 / ‑3.2 | 33 | intergenic (‑155/+288) | rrsG/clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/21); minor base C (1/0); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
GATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAC > NC_000913/2731275‑2731406 | gATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCt > 1:1126172/1‑100 (MQ=14) tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg < 1:861685/100‑1 (MQ=18) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 2:107477/1‑100 (MQ=255) tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt > 1:1082289/1‑100 (MQ=255) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt > 2:1050911/1‑100 (MQ=255) gATAAATTGTTAAAGAGCAGTGCCGCTTCACGTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg < 2:676552/95‑1 (MQ=16) tAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct > 1:1385106/1‑100 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTTAAGCATTTCTTTGCTTTtctc < 2:1054092/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 1:301051/100‑1 (MQ=255) aaaGTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTAGAGAGTCAAGCATTTCTTTGCTTTtctc < 2:618126/100‑1 (MQ=255) aTTGTTAAAGAGCAGTGCCGTTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg > 2:139908/1‑100 (MQ=255) ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt < 1:1731337/100‑1 (MQ=255) nttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATCCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 2:1854109/2‑100 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 1:1657495/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 1:1531411/1‑100 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:2037666/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 1:1049156/100‑1 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag < 1:420078/99‑1 (MQ=255) aaGAGCAGTGCCTCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt < 2:69823/100‑1 (MQ=255) aaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt > 2:2100290/1‑100 (MQ=255) agCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct < 2:343085/100‑1 (MQ=255) gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc < 2:1031281/100‑1 (MQ=255) aGTGCCGCTTCCCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg > 2:1172441/1‑100 (MQ=255) aGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg < 1:1078463/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 2:658625/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 2:567861/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 1:1074154/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 2:1105498/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 1:1712141/100‑1 (MQ=255) gTGCCGCTTCACTTGTTCTCAGCGGCGCGGCGGGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 2:1888779/100‑1 (MQ=18) tGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga > 1:721282/1‑100 (MQ=255) gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag < 2:1894901/100‑1 (MQ=255) ccGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga > 1:603633/1‑100 (MQ=255) gcttcacttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc > 2:99464/1‑100 (MQ=255) | GATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAC > NC_000913/2731275‑2731406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |