Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,751,278	G→A	32.5%	V150M (GTG→ATG)	nadK →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,278	0	G	A	32.5%	111.6 / 72.4	92	V150M (GTG→ATG)	nadK	NAD kinase
Reads supporting (aligned to +/- strand):  ref base G (27/35);  new base A (15/15);  total (42/50)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TGGAAGGCCACTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGA  >  NC_000913/2751180‑2751377                                                                                                   |                                                                                                    tGGAAGGCCACTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAgtggt                                                                                                    >  1:261294/1‑100 (MQ=255)   gAAGGCCACTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGc                                                                                                  <  1:1081270/100‑1 (MQ=255)       gCCACTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCa                                                                                              >  1:1623616/1‑100 (MQ=255)        ccACTACATCAGCGAGAAACGTTTTTTTTTTTTATTTCAAGTCTGTCAGCAAAATTGCAAGAAACACATCAGCACCGCGATAAATGAAGTGATGCTTCAt                                                                                             >  2:2118631/1‑100 (MQ=255)           cTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCa                                                                                          <  2:1622583/100‑1 (MQ=255)           cTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCa                                                                                          <  1:938646/100‑1 (MQ=255)             aCATCAGCGAGAAACGTTTTTTGCTAGAAGCGCAAGTCTGTCAACAAGATTGCCAGAAACGCATAAGCACCGCGATAAATGAAGTAGTGCTTCCTTCAg                                                                                         >  2:1908228/1‑99 (MQ=255)               aTCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGc                                                                                       <  1:314999/99‑1 (MQ=255)               aTCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCa                                                                                      >  2:385438/1‑100 (MQ=255)                 cAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCaaa                                                                                    <  2:278357/100‑1 (MQ=255)                   gCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGt                                                                                  >  1:1591062/1‑100 (MQ=255)                        aaaCGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGgcgc                                                                             <  1:1786831/100‑1 (MQ=255)                        aaaCGTTTTTTGCTGGAAGCGCAAGTCTGTAAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATTCAGGCAAAGTgg                                                                                <  2:22505/97‑1 (MQ=255)                         aaCGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCa                                                                            <  1:870882/100‑1 (MQ=255)                         aaCGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCa                                                                            >  1:149502/1‑100 (MQ=255)                         aaCGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCa                                                                            >  2:1640100/1‑100 (MQ=255)                               ttttGCTGGAAGCGGAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATg                                                                        <  1:749637/98‑1 (MQ=255)                                tttGCTGGAAGCGCGAGGCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCAGCCAGGCAAAGTGGCGCATATGAt                                                                      <  1:1564419/99‑1 (MQ=255)                                tttGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGAtt                                                                     >  2:1086700/1‑100 (MQ=255)                                 ttGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTg                                                                    <  2:1593665/100‑1 (MQ=255)                                  tGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGAttaa                                                                   >  1:754211/1‑98 (MQ=255)                                   gCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAg                                                                  <  1:1699804/100‑1 (MQ=255)                                    cTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGt                                                                 >  1:527902/1‑100 (MQ=255)                                     tGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGtt                                                                >  1:747083/1‑100 (MQ=255)                                     tGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGtt                                                                >  1:608128/1‑100 (MQ=255)                                     tGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGtt                                                                >  2:2043722/1‑100 (MQ=255)                                      ggAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTc                                                               >  1:366359/1‑100 (MQ=255)                                      ggAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTc                                                               >  2:1260725/1‑100 (MQ=255)                                      ggAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTc                                                               <  1:192782/100‑1 (MQ=255)                                        aaGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGa                                                             <  2:261295/100‑1 (MQ=255)                                          gcgcAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAg                                                           <  1:1746468/100‑1 (MQ=255)                                          gcgcAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAg                                                           <  1:2148762/100‑1 (MQ=255)                                          gcgcAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAg                                                           <  2:487193/100‑1 (MQ=255)                                            gcAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAgtg                                                         <  2:2074640/100‑1 (MQ=255)                                                gTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGtata                                                     <  2:1818218/100‑1 (MQ=255)                                                gTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGtata                                                     >  2:281013/1‑100 (MQ=255)                                                 tCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTatat                                                    <  2:528051/100‑1 (MQ=255)                                                 tCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTatat                                                    >  1:1765718/1‑100 (MQ=255)                                                  cTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATc                                                   <  2:985323/100‑1 (MQ=255)                                                   tGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATcg                                                  >  2:58115/1‑100 (MQ=255)                                                    gTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATcga                                                 >  1:303674/1‑100 (MQ=255)                                                      cAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATcgacg                                               >  1:1714454/1‑100 (MQ=255)                                                      cAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGCGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATcgacn                                               <  2:1430032/100‑2 (MQ=255)                                                        gCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACgag                                             >  1:232174/1‑100 (MQ=255)                                                           aGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATc                                          >  2:582468/1‑100 (MQ=255)                                                           aGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGAGCATATGAATGAGTTCGAAGTGTATATcgacg                                               >  2:298978/1‑95 (MQ=255)                                                             aTTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCtt                                        <  2:1148889/100‑1 (MQ=255)                                                              ttGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCttt                                       >  2:390447/1‑100 (MQ=255)                                                               tGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTg                                      <  2:1218417/100‑1 (MQ=255)                                                               tGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTg                                      <  1:947204/100‑1 (MQ=255)                                                               tGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGTAGTGTATATCGACGAGATCTTTg                                      <  2:1989966/100‑1 (MQ=255)                                                               tGCAAGGAACGCATCAGCACCGCGATAAATGAAGTGGTGCGTCAACCAGGCAAAGTGGCGCATGTGATTGAGTTTGAAGTGTATATCGACGAGATCttt                                       <  2:1776150/99‑1 (MQ=255)                                                                gCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGc                                     >  2:1892461/1‑100 (MQ=255)                                                                gCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGc                                     <  2:1107273/100‑1 (MQ=255)                                                                gCCAGAAACGCATCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGc                                     >  2:1439381/1‑100 (MQ=255)                                                                 ccAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCg                                    <  1:675953/100‑1 (MQ=255)                                                                 ccAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATACCGACGAGATCTTTGCg                                    >  1:1533444/1‑100 (MQ=255)                                                                  cAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGt                                   <  1:44021/100‑1 (MQ=255)                                                                    gAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGttt                                 <  2:232174/100‑1 (MQ=255)                                                                    gAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGttt                                 >  1:1095188/1‑100 (MQ=255)                                                                    gAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGttt                                 <  1:1207135/100‑1 (MQ=255)                                                                    gAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGttt                                 >  1:995343/1‑100 (MQ=255)                                                                     aaaCGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATGGAGTTCGAAGTGTATATCGACGAGATCTTTGCGtttt                                <  2:1196234/100‑1 (MQ=255)                                                                      aaCGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTtc                               <  1:458445/100‑1 (MQ=255)                                                                          cATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAg                           >  2:1587700/1‑100 (MQ=255)                                                                          cATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAg                           <  2:1095186/100‑1 (MQ=255)                                                                          cATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAg                           <  1:2209370/100‑1 (MQ=255)                                                                          cATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAg                           <  2:1026965/100‑1 (MQ=255)                                                                           aTCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGc                          <  1:1958221/100‑1 (MQ=255)                                                                           aTCAGCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGc                          >  2:266383/1‑100 (MQ=255)                                                                            tCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCg                         <  2:366360/100‑1 (MQ=255)                                                                               gCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATc                      >  1:2177747/1‑100 (MQ=255)                                                                               gCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATc                      >  1:270920/1‑100 (MQ=255)                                                                               gCACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATc                      <  1:35458/100‑1 (MQ=255)                                                                                cACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCt                     <  1:412919/100‑1 (MQ=255)                                                                                cACCGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCt                     <  1:1878777/100‑1 (MQ=255)                                                                                 aCCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCt                     <  1:525431/99‑1 (MQ=255)                                                                                 aCCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTg                    >  2:1087521/1‑100 (MQ=255)                                                                                  ctgcgATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGa                   >  1:161083/3‑100 (MQ=255)                                                                                  ccGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTCCGATTTTTCAGCGATCTg                    >  2:1308716/1‑99 (MQ=255)                                                                                  ccGCGATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGa                   >  2:2109952/1‑100 (MQ=255)                                                                                     cgATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATgg                <  1:751746/100‑1 (MQ=255)                                                                                     cgATAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATgg                <  1:843561/100‑1 (MQ=255)                                                                                       aTAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATACCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGAc              <  2:1533434/100‑1 (MQ=255)                                                                                        tAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATgg                >  2:146371/1‑97 (MQ=255)                                                                                        tAAATGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACt             <  1:202098/100‑1 (MQ=255)                                                                                         aaaTGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGACCTGTTGGACTa            <  2:1169920/100‑1 (MQ=255)                                                                                          aaTGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTaa           >  1:1380916/1‑100 (MQ=255)                                                                                           aTGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAat          >  2:553312/1‑100 (MQ=255)                                                                                           aTGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAat          >  2:978463/1‑100 (MQ=255)                                                                                            tGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAatt         <  1:544920/100‑1 (MQ=255)                                                                                            tGAAGTGATGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAatt         <  2:1748649/100‑1 (MQ=255)                                                                                              aaGTGGTACTTCACCCTGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCGCAGCGTTCCGATGGACTGattat       >  2:913791/1‑100 (MQ=255)                                                                                                gtgatgCTTCATCCAGGCAAAGTGGCGCATCTGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTAttt     >  2:1469988/1‑100 (MQ=255)                                                                                                gtgatgCTTCATCAAGGCAAAGTGGCGCATATGATTGACTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTAttt     >  2:1384307/1‑100 (MQ=255)                                                                                                 tggtgCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTc    <  1:899272/100‑1 (MQ=255)                                                                                                 tggtgCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTc    <  1:202647/100‑1 (MQ=255)                                                                                                  ggtgCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCg   >  1:89454/1‑100 (MQ=255)                                                                                                   gtgCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGa  <  2:1154213/100‑1 (MQ=255)                                                                                                   |                                                                                                    TGGAAGGCCACTACATCAGCGAGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAGAAACGCATCAGCACCGCGATAAATGAAGTGGTGCTTCATCCAGGCAAAGTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGA  >  NC_000913/2751180‑2751377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑