| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,856,887 | C→T | 70.1% | intergenic (‑81/‑206) | ygbA ← / → mutS | uncharacterized protein/methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,856,887 | 0 | C | T | 70.1% | 101.7 / 55.4 | 83 | intergenic (‑81/‑206) | ygbA/mutS | uncharacterized protein/methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (13/11); new base T (23/34); total (38/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTC > NC_000913/2856791‑2856982 | tACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCaa > 2:907119/1‑100 (MQ=255) cGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAAcc < 2:2103709/100‑1 (MQ=255) cTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGa > 1:1466049/1‑100 (MQ=255) gCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc < 1:1349920/100‑1 (MQ=255) aCATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGTGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAAttt < 2:217277/100‑1 (MQ=255) aCATGCTGCCTACACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAAtt < 2:2072848/99‑1 (MQ=255) cATGCTGCCTCCACCTCATTAAGATGTATATATATTACATCTTAATCTTAAAAGGCACTATGACTCCAAAGAAGAAAGGTTAGTCAACCGATACAAtttt > 2:424100/1‑100 (MQ=255) aTGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTg < 2:195522/100‑1 (MQ=255) aTGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTg < 2:737368/100‑1 (MQ=255) tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc > 1:870835/1‑100 (MQ=255) ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGTCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGt < 2:1291008/100‑1 (MQ=255) tgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTa < 1:2164778/100‑1 (MQ=255) ccTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACt < 2:758571/100‑1 (MQ=255) ccTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACt > 1:1609888/1‑100 (MQ=255) cTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACtt > 1:494480/1‑100 (MQ=255) ccACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGc < 1:159102/100‑1 (MQ=255) cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAACGGGCACTATGACTCTAAAGAAGAAGGGTTCGACAACCGCTATCATTTTGCGTACTTGCt > 2:13245/1‑100 (MQ=255) aCCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCtt > 1:1462185/1‑100 (MQ=255) aCCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCtt < 2:2153297/100‑1 (MQ=255) ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTc > 2:723912/1‑100 (MQ=255) cTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCtt < 2:955939/98‑1 (MQ=255) tCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGTAGTGTTATCCAAGCGATACAATTTTGCGTACTTGCTTCa < 2:1333230/99‑1 (MQ=255) cATTAAGATGTATTTATATTACATCTTAATCTTACAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTCCTTTCTTCATa < 2:1176454/100‑1 (MQ=255) cATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACAATGAATAAAAAAAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATa < 2:435120/100‑1 (MQ=255) aTTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATaa < 2:1884573/100‑1 (MQ=255) ttAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAg > 2:2073028/1‑100 (MQ=255) ttAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAg > 2:1867766/1‑100 (MQ=255) ttAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGACAACCGATACAATTTTGCGTACTTGCTTCATAAg > 2:1731995/1‑100 (MQ=255) ttAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATAACTCCAAAGAAGAAAGGTTAGCAAACCGATAAACTTTAGCGTACATACTTCATaa > 2:1359171/1‑99 (MQ=255) tAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGc < 2:1622655/100‑1 (MQ=255) gATGTATTTATAGTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATc > 1:1473410/1‑100 (MQ=255) aTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCa < 2:997590/100‑1 (MQ=255) tGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCAc > 2:1399419/1‑100 (MQ=255) tGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCAc > 1:529942/1‑100 (MQ=255) tATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGc > 1:1429736/1‑100 (MQ=255) tATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGc < 1:2188498/100‑1 (MQ=255) aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCa > 1:724008/1‑100 (MQ=255) aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGATTAACAAACAGATACAATTTTGCGTACTTGGTTTATAAGAATCACgaa > 2:2124249/1‑98 (MQ=255) tataTTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCaaa < 2:1857690/99‑1 (MQ=255) atatTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg < 1:1540834/100‑1 (MQ=255) tatTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGc < 1:354590/100‑1 (MQ=255) atTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGATGGGTTAGCTAACCGATACAATTTTGTGTACTTGTTTCATAAGCATCACGCAAAAGCt < 2:912274/100‑1 (MQ=255) atTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCt > 2:2227493/1‑100 (MQ=255) ttACATCTTAATCTTAAATGACACTATGACTACAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCAAGCAAAAGCTg > 2:948442/1‑100 (MQ=255) ttACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTg > 2:448203/1‑100 (MQ=255) cATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCa > 1:1881193/1‑99 (MQ=255) atcttaatcttaAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaa > 1:6878/1‑100 (MQ=255) atcttaatcttaAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaa < 1:513560/100‑1 (MQ=255) tcttaatcttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaaa > 1:1383967/1‑100 (MQ=255) atcgtaaAGGGCACTATTACTCCAAAGAAGAGGGGTTAGTCAACCTATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCa < 2:1943123/100‑1 (MQ=255) tcttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCAt > 2:1554712/1‑100 (MQ=255) cttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc < 1:435758/100‑1 (MQ=255) cttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc < 1:478796/100‑1 (MQ=255) ttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCt < 1:492051/100‑1 (MQ=255) ttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCa > 1:775757/1‑97 (MQ=255) ttaaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCt > 2:1728254/1‑100 (MQ=255) taaAGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCtt > 1:73243/1‑100 (MQ=255) aGGGCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTc < 2:1866962/99‑1 (MQ=255) gggCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc < 2:1734137/100‑1 (MQ=255) gggCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc < 1:1338487/100‑1 (MQ=255) ggCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg < 2:1609877/100‑1 (MQ=255) ggCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg > 1:254115/1‑100 (MQ=255) ggCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg < 1:809198/100‑1 (MQ=255) ggCACTATGACTCCAAAGAAGAAGGCTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg < 2:475536/100‑1 (MQ=255) gCACTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg > 2:696980/1‑100 (MQ=255) cTATGACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAAc > 1:1895336/1‑100 (MQ=255) cTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAAc > 2:1606097/1‑100 (MQ=255) gACTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc < 1:1099917/100‑1 (MQ=255) aCTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAATCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCa < 2:449517/100‑1 (MQ=255) cTCCAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTACTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt > 2:368911/1‑100 (MQ=255) ccAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTGCCGGAACCAGCATCa > 2:1553307/1‑100 (MQ=255) ccAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCa < 1:244928/100‑1 (MQ=255) ccAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACCTGCTTCATAAGCATCACGCAAAAGCTGCAACACAGCATCTTTCCCGGACCCAGCATc > 2:365981/1‑99 (MQ=255) cAAAGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCaa < 2:671933/100‑1 (MQ=255) cAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCaa > 1:1922173/1‑100 (MQ=255) aaaGAAGAAGGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAg < 1:1473098/100‑1 (MQ=255) gaagaagGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAAc < 2:619753/100‑1 (MQ=255) gaagGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCg < 2:1895324/100‑1 (MQ=255) aagGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCCTCAAGAACTCGc < 1:1469913/100‑1 (MQ=255) aagGGTTAGTCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGc < 2:1922507/100‑1 (MQ=255) gggTTAGTCACCCGATACAATTTTGCGGACTTGCCGCATGAGCATCCCGCAAAAGCTGCAAGCCAGCAGGTTTCCCGGAACCAGCATCAAGAACTCGcc < 2:795587/99‑1 (MQ=255) gTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGtt < 2:149041/100‑1 (MQ=255) tAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTc > 1:109095/1‑99 (MQ=255) | TACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTC > NC_000913/2856791‑2856982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |