Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,967,887 G→A 66.8% A183V (GCC→GTC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,967,8870GA66.8% 100.6 / 78.2 98A183V (GCC→GTC) ptsPPEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base G (15/17);  new base A (33/31);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTG  >  NC_000913/2967788‑2967983
                                                                                                   |                                                                                                
aGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCgg                                                                                                  <  2:134360/100‑1 (MQ=255)
aGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCgg                                                                                                  >  1:1638387/1‑100 (MQ=255)
 gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGc                                                                                                 >  1:1016135/1‑100 (MQ=255)
  cgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCa                                                                                                >  1:515455/1‑100 (MQ=255)
   gcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACaa                                                                                               >  1:278450/1‑100 (MQ=255)
      ccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACATGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCg                                                                                            <  2:1238078/100‑1 (MQ=255)
        ggTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGcc                                                                                          <  2:1016133/100‑1 (MQ=255)
        ggTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGcc                                                                                          <  1:1867769/100‑1 (MQ=255)
           cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCcaca                                                                                       <  2:1617537/100‑1 (MQ=255)
            aGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGacacac                                                                                      >  1:135611/1‑100 (MQ=255)
            aGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCacac                                                                                      >  2:1394242/1‑100 (MQ=255)
               cGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAACCTTCGACAATCGCCACACCAg                                                                                   >  1:887272/1‑100 (MQ=255)
                 ttCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGt                                                                                 <  2:57318/100‑1 (MQ=255)
                 ttCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGt                                                                                 <  2:383767/100‑1 (MQ=255)
                  tCGGGTTCCAGAGCGGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAATGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGtg                                                                                <  1:306565/100‑1 (MQ=255)
                  tCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGtg                                                                                >  2:977894/1‑100 (MQ=255)
                  tCGCGTTCCAGAGCCCAATCCACCGTAGATGCCTTATACACCTGTTCCATTAAATGTAACGTGGCATCCTGCTAGCCTTCGACAATCGCCAAACCAGGtg                                                                                <  2:462478/100‑1 (MQ=255)
                   cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGtgc                                                                               >  1:1492401/1‑100 (MQ=255)
                   cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGtgc                                                                               >  2:1174749/1‑100 (MQ=255)
                   cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGtgc                                                                               <  2:1549764/100‑1 (MQ=255)
                     cgTTCCCGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGAtgctg                                                                             >  2:442005/1‑100 (MQ=255)
                     cgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGCCAATCGCCACACCAGGtgctg                                                                             >  2:1444951/1‑100 (MQ=255)
                      gTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGtgctgc                                                                            >  1:1829986/1‑100 (MQ=255)
                       ttCCAGAGCCGGATCCAGCGTTGATGCATGATACACCTGTTCCATTAAAGGTCACGTGGCATCCTGCCAACCTTAGTCAATAGCCACACCACGt                                                                                 >  2:159274/1‑94 (MQ=255)
                         ccAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCgg                                                                         >  2:272176/1‑100 (MQ=255)
                           agagCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTa                                                                       <  2:251002/100‑1 (MQ=255)
                            gagCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTaa                                                                      >  1:2067724/1‑100 (MQ=255)
                              gCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATg                                                                    >  1:397132/1‑100 (MQ=255)
                                cGGATCCAGCGTTGATGCCTGATACACTTGTTCCATTAAAGGTAACGTGGTATCCTGCCATCCTTCGGAAATCGCCATACCAGGTGCTGCCGGTAATg                                                                    <  2:698540/98‑1 (MQ=255)
                                cGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGTTGCCGGTAATgcg                                                                  <  2:739075/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                                <  1:133417/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                                <  1:1138706/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                                >  1:833569/1‑100 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                                <  2:1137903/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                                <  1:888541/100‑1 (MQ=255)
                                    tCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGa                                                              <  2:107403/100‑1 (MQ=255)
                                    tCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGa                                                              <  2:278451/100‑1 (MQ=255)
                                      cAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGAtt                                                            >  1:419131/1‑100 (MQ=255)
                                      cAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGAtt                                                            >  2:527059/1‑100 (MQ=255)
                                      cAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGAtt                                                            >  2:1857080/1‑100 (MQ=255)
                                       aGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTc                                                           <  2:501208/100‑1 (MQ=255)
                                         cGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCAAACCAAGTGCTGACAGTAATACAAGAATTcgc                                                         >  1:2004123/1‑100 (MQ=255)
                                          gTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgcg                                                        <  2:1638376/100‑1 (MQ=255)
                                             gATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCt                                                     >  2:2121325/1‑100 (MQ=255)
                                                gCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATTCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGc                                                  <  2:469906/100‑1 (MQ=255)
                                                 ccTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCt                                                     <  1:2121339/96‑1 (MQ=255)
                                                 ccTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCg                                                 >  1:19433/1‑100 (MQ=255)
                                                  cTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATTCGCGGATTCGCGTCTGGCGa                                                <  2:1060983/100‑1 (MQ=255)
                                                  cTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGa                                                <  2:1824010/100‑1 (MQ=255)
                                                        cacCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                          >  1:1054841/1‑100 (MQ=255)
                                                        cacCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                          >  2:181808/1‑100 (MQ=255)
                                                         acCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGcc                                         <  2:1203846/100‑1 (MQ=255)
                                                         acCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTgac                                         <  1:1520719/100‑3 (MQ=255)
                                                          ccTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCATACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                          <  2:155660/98‑1 (MQ=255)
                                                            tGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCaa                                      >  1:2094238/1‑100 (MQ=255)
                                                             gTTCCATTACAGGTAACGTACCATCCTGCCAGCCTTCGACACTCGCCACACCAGGTGCTGCCGGTAATGCACTGATTCGCGTCTGGCGATATTGCCCaaa                                     >  2:356009/1‑100 (MQ=255)
                                                              ttccgatctaGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAAc                                    <  1:225687/91‑1 (MQ=255)
                                                               tCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACa                                   <  1:899531/100‑1 (MQ=255)
                                                               tCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACa                                   >  2:1334294/1‑100 (MQ=255)
                                                                ccATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACaa                                  >  2:18143/1‑100 (MQ=255)
                                                                 cATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACaa                                  <  2:673814/99‑1 (MQ=255)
                                                                 cATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAg                                 >  2:2225660/1‑100 (MQ=255)
                                                                 cATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAg                                 <  1:1786476/100‑1 (MQ=255)
                                                                 cATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAg                                 >  1:2221356/1‑100 (MQ=255)
                                                                 cATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAg                                 >  1:784451/1‑100 (MQ=255)
                                                                 cATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAg                                 >  1:1223560/1‑100 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAgg                                <  1:166536/100‑1 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAgg                                <  1:429464/100‑1 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAgg                                <  2:1317950/100‑1 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGc                               <  1:2022434/100‑1 (MQ=255)
                                                                     aaaGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAg                             <  1:181808/100‑1 (MQ=255)
                                                                      aaGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACCTCAGGTGCTGCCGGTACTGCGCGGATTCACGTCTCCCGATATTACCCAAACAAGGCa                              >  1:126580/1‑98 (MQ=255)
                                                                      aaGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGt                            >  2:15505/1‑100 (MQ=255)
                                                                      aaGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGt                            >  1:434455/1‑100 (MQ=255)
                                                                       aGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACagatcggaa                           >  2:225687/1‑92 (MQ=255)
                                                                       aGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTc                           >  2:1027756/1‑100 (MQ=255)
                                                                        ggTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGTGTCTGGCGATATTGCCCTAACAAGGCAGtga                          <  1:1508828/100‑3 (MQ=255)
                                                                         gTAACGTGGCATCCTGCCAGCCTTCGACAATAGCCACACCAGATGCTCCCGGTAATGCGCGGAATCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCaa                         >  2:898917/1‑100 (MQ=255)
                                                                           aaCGTGGCATCCTGCCAGCCGTCGGCAATCGCCACACCAGGTGCTTCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAAc                        <  2:472676/99‑1 (MQ=255)
                                                                            aCGTGGCATCCTGCCAGCCTTCGGCAATCGACACACCAGGTGCTGCCGGTAATGCGCGGATTCGCATCTGGCGATATTGCCCAAACAAGGCATTCAACt                       >  2:200100/1‑99 (MQ=255)
                                                                             gctggcaggatGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGc                     >  1:1494646/11‑100 (MQ=255)
                                                                               tGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGa                   >  1:1546295/1‑100 (MQ=255)
                                                                                 gCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACt                 >  2:263461/1‑100 (MQ=255)
                                                                                   aTCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTga               <  2:122349/100‑1 (MQ=255)
                                                                                    tCCTGCCAGCCTTCGGCAATCACCACACCAGGAGCTGCCGGTAATGCTCGGATTCGCGTCTGGCAAAATTGCACAAACAAGGCAGTCAACTGCGAc                  >  2:424404/1‑96 (MQ=255)
                                                                                    tCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTgag              <  1:2002552/100‑1 (MQ=255)
                                                                                     ccTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTgaga             <  1:15076/100‑1 (MQ=255)
                                                                                       tGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaa           >  1:1689144/1‑100 (MQ=255)
                                                                                       tGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaa           >  1:216902/1‑100 (MQ=255)
                                                                                        gCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaag          <  2:1025761/100‑1 (MQ=255)
                                                                                        gCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaag          >  1:733290/1‑100 (MQ=255)
                                                                                          cAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaagaa        >  2:2213308/1‑100 (MQ=255)
                                                                                           aGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt       <  1:1644077/100‑1 (MQ=255)
                                                                                           aGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAACGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaag          <  2:1902335/97‑1 (MQ=255)
                                                                                           aGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt       <  2:43853/100‑1 (MQ=255)
                                                                                            gCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATa      <  2:1640441/100‑1 (MQ=255)
                                                                                              cTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGc    >  1:393585/1‑100 (MQ=255)
                                                                                              cTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGc    >  1:1798527/1‑100 (MQ=255)
                                                                                               ttCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt       <  1:885179/96‑1 (MQ=255)
                                                                                                tCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGGGAAAGAATAGCTg  <  2:713876/100‑1 (MQ=255)
                                                                                                   |                                                                                                
AGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTG  >  NC_000913/2967788‑2967983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: