| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,338,374 | T→C | 36.9% | N30D (AAC→GAC) | mlaD ← | OM lipid asymmetry maintenance protein; membrane‑anchored ABC family periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,338,374 | 0 | T | C | 36.9% | 59.3 / 78.1 | 80 | N30D (AAC→GAC) | mlaD | OM lipid asymmetry maintenance protein; membrane‑anchored ABC family periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (25/25); new base C (17/12); total (43/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATT > NC_000913/3338278‑3338471 | caccaATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCcgc > 1:650661/1‑100 (MQ=255) ccaATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTcgccg > 1:2051977/1‑100 (MQ=255) ccaATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTcgccg > 1:156330/1‑100 (MQ=255) aaTACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTcgccg < 1:144732/98‑1 (MQ=255) aaTACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCgccgcc < 2:1416280/100‑1 (MQ=255) aaTACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCgccgcc > 2:374182/1‑100 (MQ=255) tACTGACCGGAGAGCGCGCTTTCAGGCCGCCTATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCtt < 2:576591/100‑1 (MQ=255) aCTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTc < 1:17475/100‑1 (MQ=255) tGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAg < 1:345546/100‑1 (MQ=255) naCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGATCGGTTCAGTCGGTATATACGTCACGTACGACGCCTTCAgg > 2:2057874/2‑100 (MQ=255) gACTGGAGAGCGCGCTTTCAGGCCGCCAATGTTAGCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAg < 1:1316692/99‑1 (MQ=255) gACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAgg < 1:1616849/100‑1 (MQ=255) gACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAg < 1:276487/99‑1 (MQ=255) aCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGc > 2:1465541/1‑100 (MQ=255) gcgcgcTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCaaacaaac > 1:1391976/1‑100 (MQ=255) gcgcGTTCAGGCCGCCAATGTTAGCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAaacaaaca < 1:711883/99‑1 (MQ=255) tttCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACTTTCGCCGCCTTTAGTCAAACAAACAGcgcc < 2:383476/100‑1 (MQ=255) tttCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGcgcc < 2:1104523/100‑1 (MQ=255) tCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGcgccgc < 2:1692365/100‑1 (MQ=255) tCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATAGACTTAAAATCCGACGCCTTCAGGCAAACAAACAGcgccgc > 2:817375/1‑100 (MQ=255) ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1285487/1‑100 (MQ=255) ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1713487/1‑100 (MQ=255) ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1827045/1‑100 (MQ=255) ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1402565/1‑100 (MQ=255) ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAACGCCGCcag > 1:576948/1‑100 (MQ=255) gccgccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcagc > 1:1356091/1‑100 (MQ=255) ccgccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGTCTTCAGGCAAACAAACAGCGCCGCcagca > 1:87662/1‑100 (MQ=255) ccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtg > 1:5608/1‑100 (MQ=255) ccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtg > 1:221484/1‑100 (MQ=255) aaTGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtgc < 1:289620/99‑1 (MQ=255) tGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtgctgc < 2:438250/100‑1 (MQ=255) gTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTgctgct < 2:2051963/100‑1 (MQ=255) ttATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTgctgct < 1:776956/99‑1 (MQ=255) tCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCtaata < 1:290438/100‑1 (MQ=255) cGAACGTCGCATAAAGCGTGTAGGTCGGTTAAGTACGTATGGACGTAACGTCCGCCGCCCTCAGGCAAACAAACAGCGCCGCCAGCAGTCCTGCtaataa > 2:1071775/1‑100 (MQ=255) gAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaa > 1:1234448/1‑100 (MQ=255) gAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaa < 2:1632905/100‑1 (MQ=255) aCGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaaaa < 2:143023/100‑1 (MQ=255) aCGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaaaa < 1:957821/100‑1 (MQ=255) cGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAg > 2:1814487/1‑100 (MQ=255) cGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAg < 1:53075/100‑1 (MQ=255) gTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAATCAGCGCCGCCAGCAGTGCTGCTAATAAAAAGa < 1:2132587/100‑1 (MQ=255) gTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGa < 2:255045/100‑1 (MQ=255) cGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATa > 1:886066/1‑100 (MQ=255) gCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCGGCAGTGCTGCTAATAAAAAGATac < 2:401742/100‑1 (MQ=255) gCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATac < 1:1389609/100‑1 (MQ=255) gCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATac > 1:308665/1‑100 (MQ=255) aTAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATaccc < 1:1415347/100‑1 (MQ=255) aTAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATaccc > 1:1598030/1‑100 (MQ=255) aaGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATacccacc < 2:1455490/100‑1 (MQ=255) gCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATAcccaccca > 1:81772/1‑100 (MQ=255) cGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCaa > 2:1055118/1‑100 (MQ=255) gtAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCaaagt < 2:654113/100‑3 (MQ=255) tAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAAttt > 2:1773399/1‑100 (MQ=255) aGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTc > 1:52465/1‑100 (MQ=255) aGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTc < 1:2019883/100‑1 (MQ=255) aGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTc > 2:1883084/1‑100 (MQ=255) ggTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCa > 1:2051651/1‑100 (MQ=255) ggTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCa > 1:909310/1‑100 (MQ=255) tCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCAtt < 1:164336/100‑1 (MQ=255) ggTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTGAttt > 1:1027512/1‑99 (MQ=255) ggTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCAtttt > 1:1700968/1‑100 (MQ=255) tCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCAttttttt > 2:185665/1‑100 (MQ=255) cAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCACCAGTGCTGATAATAAAAAGAAACCCACCCGAATTTCAtttttttt > 1:1333428/1‑100 (MQ=255) gTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTc > 2:472015/1‑99 (MQ=255) tACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGt < 1:1492700/100‑1 (MQ=255) aCGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATCCCCACCCAAATTTCATTTTTTTTCGtt < 2:1744483/100‑1 (MQ=255) cGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGttt < 1:1391375/100‑1 (MQ=255) tATGGACGTCACGTTGGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCTCCCAAATTTCATTTTTTTTCGttgg < 2:179113/99‑3 (MQ=255) tATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGc > 2:1008502/1‑100 (MQ=255) ggACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCTTTTGCATg > 2:609558/1‑100 (MQ=255) ggACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCAt > 1:771511/1‑99 (MQ=255) ggACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATg > 2:203454/1‑100 (MQ=255) gACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGa > 2:1498029/1‑100 (MQ=255) gACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGTTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGa < 2:1596079/100‑1 (MQ=255) cGTCACGTTCGCCGCCTTCAGGCAAACAAACCGCGCCGCCAGCAGTGCTGCTAATAAAAAGAAACCCACCCAAATTACAATTTTTTTCGTTTGCATGaa > 1:112275/1‑99 (MQ=255) cGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc > 2:711562/1‑100 (MQ=255) cGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc < 1:850499/100‑1 (MQ=255) cGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc < 2:81772/100‑1 (MQ=255) gTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAAtt > 1:955522/1‑100 (MQ=255) | CACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATT > NC_000913/3338278‑3338471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |