Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,420,599 C→T 31.0% Q156* (CAG→TAG)  yhdX → putative amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,420,5990CT31.0% 119.8 / 65.7 90Q156* (CAG→TAG) yhdXputative amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (31/31);  new base T (14/14);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

TTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGG  >  NC_000913/3420502‑3420695
                                                                                                 |                                                                                                 
ttCCACAATTTATATTGAGATCTTCCGTTATATGCCCGCGCTGCTGCAAATCTTCTTCTGGTACTTTGTCTTGTTGCGCAATTTACCCGGACCACGCCAg                                                                                                 <  2:900479/100‑1 (MQ=255)
 tCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAgg                                                                                                >  2:575380/1‑100 (MQ=255)
  ccACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGc                                                                                               >  1:2020229/1‑100 (MQ=255)
  ccACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGc                                                                                               >  2:991146/1‑100 (MQ=255)
   cacaATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCt                                                                                              >  1:1972679/1‑100 (MQ=255)
    acaATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCtg                                                                                             <  1:562281/100‑1 (MQ=255)
      aaTTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCtgtg                                                                                           <  2:722513/100‑1 (MQ=255)
        tttATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAg                                                                                         >  1:255337/1‑100 (MQ=255)
          tataTTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAgcg                                                                                       >  1:1130729/1‑100 (MQ=255)
             atTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGt                                                                                    >  2:1925813/1‑100 (MQ=255)
             atTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGt                                                                                    >  2:1564084/1‑100 (MQ=255)
             agtGAGATCTTCCATAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGt                                                                                    >  1:1522882/3‑100 (MQ=255)
               tGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGGGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGttt                                                                                  >  2:1085918/1‑100 (MQ=255)
                 agaTCTTCCGTACTATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCCGGCTGTGAGCGCGTTTg                                                                                 >  2:1450832/1‑99 (MQ=255)
                   aTCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGTTACTTTGCCGTGTTGCGCTATTTGCCCGGACCTTTCTAGGCTGTGAGAGCGTTTg                                                                                 <  2:935044/97‑1 (MQ=255)
                   aTCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATc                                                                              <  2:1496327/100‑1 (MQ=255)
                    tCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATc                                                                              <  1:338570/99‑1 (MQ=255)
                     cTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTg                                                                            <  2:1667153/100‑1 (MQ=255)
                      ttCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATGTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg                                                                           <  1:510107/100‑1 (MQ=255)
                        ccGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGcc                                                                         <  2:771624/100‑1 (MQ=255)
                         cGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCt                                                                        >  2:1845028/1‑100 (MQ=255)
                           tAATATTCCCCCGCTGCTGCAAATCTTATTCTGGTACTTAGCCGTGTTGCGCAATTTGCCCGCAGCAAGCCCGACTGTGGGCGCGTTTGATCTGGc                                                                          <  2:120071/96‑1 (MQ=255)
                            aaTATTTCCCCGCTGCTGCAAATCGTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGACCTGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCtttt                                                                     <  2:533553/100‑1 (MQ=255)
                            aaTATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCtttt                                                                     <  2:2023047/100‑1 (MQ=255)
                              tatTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTg                                                                   <  2:1522872/100‑1 (MQ=255)
                              tatTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTg                                                                   <  1:431174/100‑1 (MQ=255)
                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGa                                                                  <  2:363241/100‑1 (MQ=255)
                                ttCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAg                                                                 <  1:1273902/100‑1 (MQ=255)
                                 tCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGc                                                                <  1:575379/100‑1 (MQ=255)
                                  cccccGCTGCTGCCAATCTTCTTCTCGTACTTTGCCGTGTTTCGCAACTTGCCCGGACCACGCCTGGCTGTGAGCTCGTTTGATCTGGCCtcattg                                                                   >  2:85646/1‑91 (MQ=255)
                                  cccccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCa                                                               >  2:152716/1‑100 (MQ=255)
                                   ccccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGACTTTTTGAGCaa                                                              >  2:2117690/1‑100 (MQ=255)
                                      cGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATc                                                            >  2:1166305/1‑99 (MQ=255)
                                        ctgctgCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGt                                                          >  1:870385/1‑99 (MQ=255)
                                        ctgctgCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTg                                                         <  2:481953/100‑1 (MQ=255)
                                        ctgctgCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTg                                                         <  1:2099543/100‑1 (MQ=255)
                                          gctgcAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGCTCTGGCCTTTTTGAGCAATCGTgg                                                        >  1:1103063/1‑99 (MQ=255)
                                           ctgcAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGc                                                      <  1:546471/100‑1 (MQ=255)
                                             gcAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCtt                                                    <  1:685462/100‑1 (MQ=255)
                                             gcAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCtt                                                    >  2:116166/1‑100 (MQ=255)
                                              cAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCttt                                                   <  2:1361664/100‑1 (MQ=255)
                                              cAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCttt                                                   <  1:1023/100‑1 (MQ=255)
                                              cAAATCTTCTTATGGTACTTTGCAGTGTTGAGCAATTTGCCTGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCttt                                                   >  2:1842476/1‑100 (MQ=255)
                                               aaaTCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTta                                                  >  2:247354/1‑100 (MQ=255)
                                               aaaTCTTCTTCTGGTACTTTGCAGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCACTCGTGGGCTTta                                                  >  2:1277035/1‑100 (MQ=255)
                                                 aTCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTtata                                                >  2:1241498/1‑100 (MQ=255)
                                                    ttcttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTc                                             >  2:914369/1‑100 (MQ=255)
                                                    ttcttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTc                                             >  1:1417444/1‑100 (MQ=255)
                                                     tcttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTcc                                            <  2:916750/100‑1 (MQ=255)
                                                     tcttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTcc                                            >  2:1319271/1‑100 (MQ=255)
                                                      cttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCg                                           <  2:575727/100‑1 (MQ=255)
                                                      cttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCg                                           >  2:1650115/1‑100 (MQ=255)
                                                      cttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCg                                           >  1:389591/1‑100 (MQ=255)
                                                       ttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGt                                          <  1:586788/100‑1 (MQ=255)
                                                        tctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTc                                         >  1:1396540/1‑100 (MQ=255)
                                                         ctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCa                                        <  2:1090880/100‑1 (MQ=255)
                                                         ctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACACCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCa                                        >  2:1376894/1‑100 (MQ=255)
                                                          tGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGTGCATTTGTGTGCTTTATATTCCGTCAc                                       >  2:2192889/1‑100 (MQ=255)
                                                           ggTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCAcc                                      >  1:1419620/1‑100 (MQ=255)
                                                             tACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGc                                    <  1:1151355/100‑1 (MQ=255)
                                                              aCTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCa                                   <  2:109130/100‑1 (MQ=255)
                                                               cTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAg                                  >  1:1436691/1‑100 (MQ=255)
                                                                 ttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCt                                <  1:84418/100‑1 (MQ=255)
                                                                 ttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCt                                >  1:1931360/1‑100 (MQ=255)
                                                                    ccGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTggg                            <  2:648110/100‑1 (MQ=255)
                                                                    ccGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTggg                            <  1:1357477/100‑1 (MQ=255)
                                                                     cGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGga                           <  1:1228123/100‑1 (MQ=255)
                                                                      gtgtTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgag                          >  1:1767227/1‑100 (MQ=255)
                                                                       tgtTGCGCAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGga                           <  2:1244788/98‑1 (MQ=255)
                                                                         ttGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACg                       >  2:2117003/1‑100 (MQ=255)
                                                                           gcgcAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTTGGAGACGGa                     >  1:771213/1‑100 (MQ=255)
                                                                           gcgcAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGa                     >  2:1645271/1‑100 (MQ=255)
                                                                           gcgcAATTTGCCCGGACCACACTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGa                     <  1:1859182/100‑1 (MQ=255)
                                                                            cgcAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGAt                    >  2:244979/1‑100 (MQ=255)
                                                                            cgcAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGATTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGAt                    <  1:2138948/100‑1 (MQ=255)
                                                                              cAATTTGCCCGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCTCCCAGCTGCGGTGACGGaata                  <  1:1140390/100‑4 (MQ=37)
                                                                              cAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGAttt                  >  2:435389/1‑100 (MQ=255)
                                                                                   tGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCTCCCAGCTGCGGTGACGGaatataaag             <  1:1942173/100‑9 (MQ=25)
                                                                                    gCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCTCCCAGCTGCGGTGACGGaatataaagc            <  1:1448396/100‑10 (MQ=25)
                                                                                    gCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCg            >  1:1750156/1‑100 (MQ=255)
                                                                                      ccGGACCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGtt          >  1:1685756/1‑100 (MQ=255)
                                                                                      ccGGACCACGCCAGGCTGGGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATTTTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGt           <  2:1393391/99‑1 (MQ=255)
                                                                                       cGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGttt         >  2:1042228/1‑100 (MQ=255)
                                                                                       cGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGttt         <  2:1121757/100‑1 (MQ=255)
                                                                                          acgaCGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCGGACACCGCAGCTGGGAGACGGATTTATTGCCTTTAta      >  2:541033/4‑99 (MQ=255)
                                                                                          aCCACGCTAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATc      <  1:1264178/100‑1 (MQ=255)
                                                                                            cACGCTAGGCTGTGAGCGCGTTTGATCTGACCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATcc     <  2:359960/99‑1 (MQ=255)
                                                                                             aCGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTg   <  2:1882898/100‑1 (MQ=255)
                                                                                             aCGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTg   <  2:1280274/100‑1 (MQ=255)
                                                                                              cGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTgg  >  1:533023/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
TTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGG  >  NC_000913/3420502‑3420695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: