Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,076 T→C 29.1% A337A (GCA→GCG ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,0760TC29.1% 130.2 / 77.1 104A337A (GCA→GCGftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (35/38);  new base C (15/15);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 106 total aligned reads displayed.

CTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACT  >  NC_000913/3600978‑3601175
                                                                                                  |                                                                                                   
ctttccctgcaatgggttccgtagcagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTg                                                                                                    >  1:1121554‑M2/36‑100 (MQ=255)
CTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTg                                                                                                    >  1:1472945‑M1/1‑100 (MQ=255)
CTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTg                                                                                                    >  2:1396518‑M1/1‑100 (MQ=255)
 tttccctgcaatgggttccgtagcagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                                                   >  1:1057785‑M2/35‑100 (MQ=255)
 tttccctgcaatgggttccgtagcagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                                                   >  1:1057754‑M2/35‑100 (MQ=255)
 TTTCCCTGCTACGTAACTCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTg                                                                                                    >  1:300132‑M1/1‑99 (MQ=255)
 TTTCCCTGCTACGGAACCCATTGAAGGGAAAGAGTATAACACGATTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTTGCAAGCCACACTGc                                                                                                   <  2:2051979‑M1/100‑1 (MQ=255)
  ttccctgcaatgggttccgtagcagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGcc                                                                                                  >  1:2015628‑M2/34‑100 (MQ=255)
  ttccctgcaatgggttccgtagcagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGcc                                                                                                  >  1:1677245‑M2/34‑100 (MQ=255)
  ttccctgcaatgggttccgtagcagggaaagagTATAACACACTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGcc                                                                                                  >  2:1222402‑M2/34‑100 (MQ=255)
  TTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGcc                                                                                                  <  1:1824673‑M1/100‑1 (MQ=255)
   tccctgcaatgggttacgtagcagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCa                                                                                                 >  2:952972‑M2/33‑100 (MQ=255)
    CCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCAc                                                                                                >  2:79514‑M1/1‑100 (MQ=255)
    CCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCAc                                                                                                >  1:81976‑M1/1‑100 (MQ=255)
     CCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCAcc                                                                                               >  1:2063905‑M1/1‑100 (MQ=255)
        GCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAg                                                                                            <  1:933572‑M1/100‑1 (MQ=255)
        GCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAg                                                                                            >  1:441901‑M1/1‑100 (MQ=255)
        GCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAg                                                                                            <  2:260733‑M1/100‑1 (MQ=255)
         CTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGc                                                                                           >  2:882363‑M1/1‑100 (MQ=255)
          tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGcc                                                                                          <  1:866626/100‑1 (MQ=255)
          tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGcc                                                                                          >  2:95647/1‑100 (MQ=255)
           aCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCa                                                                                         >  1:780987/1‑100 (MQ=255)
            cGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCaa                                                                                        <  2:133640/100‑1 (MQ=255)
              gAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatc                                                                                      >  2:1645855/1‑100 (MQ=255)
              gAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAatc                                                                                      >  2:1565545/1‑100 (MQ=255)
               aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatca                                                                                     <  1:643187/100‑1 (MQ=255)
                ncccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcat                                                                                    >  1:929656/2‑100 (MQ=255)
                 cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                                                   <  1:1673533/100‑1 (MQ=255)
                    aTTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                                                <  2:2187272/100‑1 (MQ=255)
                    aTTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                                                <  2:619905/100‑1 (MQ=255)
                    aTTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAg                                                                                >  2:821621/1‑100 (MQ=255)
                     ttGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                                               <  1:1878147/100‑1 (MQ=255)
                     ttGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                                               <  2:1591884/100‑1 (MQ=255)
                        cagggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACTGTGGCAAGCCACGCCGCCAACCAGCCAATCATCGAGCATa                                                                            >  2:1582747‑M2/12‑100 (MQ=255)
                          gggaaagagTATAACACGCTTTTATTCTTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATAcc                                                                          >  1:1362012‑M2/10‑100 (MQ=255)
                          gggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATAcc                                                                          >  2:2225502‑M2/10‑100 (MQ=255)
                          gggaaagagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATAcc                                                                          <  1:2031066‑M2/91‑1 (MQ=255)
                               agagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                                                     >  2:429941‑M2/5‑100 (MQ=255)
                               agagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                                                     >  1:1602814‑M2/5‑100 (MQ=255)
                                gagTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCagcagc                                                                    <  1:1449613‑M2/97‑1 (MQ=255)
                                   tataACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAAt                                                                 <  2:1938715/100‑1 (MQ=255)
                                    ataACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATa                                                                >  1:2220534/1‑100 (MQ=255)
                                     taACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAg                                                               <  1:334467/100‑1 (MQ=255)
                                     taACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAAGTTGTACCGTGCCCAGCCCCGCTGCCACCCAGCCAATCATCGAGCATACAAGCAGCAAtcg                                                               <  2:661278/100‑3 (MQ=255)
                                       acacGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                                                             >  2:2149765/1‑100 (MQ=255)
                                        cacGCTTTTATTATTCAGGCGTAAAGTGGCTTAAAGGTTTAACCGTGGCAAGCCACGCCGCCACGCTGCCAATCATCTAGCATTCCAGCAGCAATAGc                                                              <  2:1641800/98‑1 (MQ=255)
                                          cGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGc                                                          <  2:106867/100‑1 (MQ=255)
                                             ttttATTATTCAGGCGTAAAGTGACGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGGATACCAACAGCAATAGCAGGCa                                                         >  2:1415410/1‑98 (MQ=255)
                                              tttATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTc                                                      >  2:2194185/1‑100 (MQ=255)
                                               ttattaTTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCa                                                     <  1:1141862/100‑1 (MQ=255)
                                                tattatTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCAt                                                    >  2:2222042/1‑100 (MQ=255)
                                                   tattCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGa                                                 <  2:840298/100‑1 (MQ=255)
                                                    attCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGaa                                                <  2:49292/100‑1 (MQ=255)
                                                     ttCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                                               >  1:1190887/1‑100 (MQ=255)
                                                     ttCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                                               <  2:2089126/100‑1 (MQ=255)
                                                     ttCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                                               <  2:1495948/100‑1 (MQ=255)
                                                     ttCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                                               <  1:2177865/100‑1 (MQ=255)
                                                      tCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATg                                              <  1:1625385/100‑1 (MQ=255)
                                                      tCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATg                                              <  2:1483076/100‑1 (MQ=255)
                                                      tCAGGCATAGAGTGGCGTAAATGTTGGGCCGTTGCAAGCCACGCTGCTCTCCAGCCAATCATCGAGCATACCAGCTGCAATATCAGGCATTCATCGAATg                                              <  2:1481142/100‑1 (MQ=255)
                                                         ggCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATa                                           >  1:1626585/1‑100 (MQ=255)
                                                         ggCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATa                                           <  1:1214601/100‑1 (MQ=255)
                                                         ggCGTAAAGTGGCGTAAATGTTGTACAGTGGCAAGCAACGCGGCCACACAGCCAATCATCGAGCATACCCGCAGCAATAGCAGGCATTCATCgca                                                >  2:2207676/1‑93 (MQ=255)
                                                           cGTAAAGTGGCGTAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc                                        <  2:2126232/100‑1 (MQ=255)
                                                            gTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc                                        <  1:2225517/100‑1 (MQ=255)
                                                                aGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCAtt                                    <  1:926368/100‑1 (MQ=255)
                                                                  tGGCGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAACCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGa                                  <  1:1872530/100‑1 (MQ=255)
                                                                   ggCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGat                                 >  2:729741/1‑100 (MQ=255)
                                                                    gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACGCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGata                                <  2:447355/100‑1 (MQ=255)
                                                                    gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGata                                >  2:1105325/1‑100 (MQ=255)
                                                                    gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGata                                <  1:548042/100‑1 (MQ=255)
                                                                     cGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGatat                               <  1:2236351/100‑1 (MQ=255)
                                                                     cGTAAATGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGatat                               >  1:1719532/1‑100 (MQ=255)
                                                                      gTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATc                              >  1:1138873/1‑100 (MQ=255)
                                                                       tAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCa                             <  2:1677234/100‑1 (MQ=255)
                                                                        aaaTGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCaa                            <  1:95647/100‑1 (MQ=255)
                                                                        aaaTGTTGTACCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCaa                            >  2:64801/1‑100 (MQ=255)
                                                                          aTGTTGTACCGTGGCAAGCCACGCTGCCCCCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAAc                          <  2:214657/100‑1 (MQ=255)
                                                                           tgttgtACCGTGGCAAGTCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACt                         >  2:543783/1‑100 (MQ=255)
                                                                            gttgtACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACtt                        >  2:811536/1‑100 (MQ=255)
                                                                               gtACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGt                     <  1:1972308/100‑1 (MQ=255)
                                                                               gtACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGt                     >  1:1331872/1‑100 (MQ=255)
                                                                                caCCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGtt                    >  2:1890899/2‑100 (MQ=255)
                                                                                 aCCGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTc                       >  1:1803606/1‑96 (MQ=255)
                                                                                  ccGTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTcc                  <  1:589014/100‑1 (MQ=255)
                                                                                    gTGGCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGa                >  1:2217523/1‑100 (MQ=255)
                                                                                     tggcttgCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGaa               >  1:1701240/7‑100 (MQ=255)
                                                                                       gCAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGaaaa             >  2:1641802/1‑100 (MQ=255)
                                                                                        cAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAc            <  1:1864313/100‑1 (MQ=255)
                                                                                        cAAGCCACGCCGCGACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAc            >  2:1387624/1‑100 (MQ=255)
                                                                                        cAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCATCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAc            <  2:950721/100‑1 (MQ=255)
                                                                                        cAAGCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAc            <  1:429293/100‑1 (MQ=255)
                                                                                           gcgaCGCTGCCACCGAGCCAATCATCGAGCATACCACCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAcc           <  2:922440/95‑1 (MQ=255)
                                                                                           gCCACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCt          >  1:869816/1‑99 (MQ=255)
                                                                                             cACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCtgtg       <  2:2015614/100‑1 (MQ=255)
                                                                                             cACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCtgtg       <  2:949061/100‑1 (MQ=255)
                                                                                             cACGCCGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCtgtg       >  1:2190512/1‑100 (MQ=255)
                                                                                               cGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGcc     <  1:416606/100‑1 (MQ=255)
                                                                                                 cTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCAc   <  1:1645866/100‑1 (MQ=255)
                                                                                                  tGCCACCCCGCCAATCATCGAGCATACAAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATCGATATAAAACTTCGATCCGAAAACCTGTGCCACt  >  2:92577/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACT  >  NC_000913/3600978‑3601175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: