| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,946,246 | G→A | 23.0% | noncoding (2543/2904 nt) | rrlC → | 23S ribosomal RNA of rrnC operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,946,246 | 0 | G | A | 23.0% | 115.2 / 24.6 | 60 | noncoding (2543/2904 nt) | rrlC | 23S ribosomal RNA of rrnC operon |
| Reads supporting (aligned to +/- strand): ref base G (25/21); new base A (2/12); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGT > NC_000913/3946148‑3946325 | gggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAgg > 1:472920/1‑100 (MQ=32) ggATAACAGGCCGATACCGCCCAAGAGTTCATATCGGCGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAggg < 1:1336690/100‑1 (MQ=12) gATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGt > 1:157221/1‑100 (MQ=32) aTAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGt < 1:1101717/99‑1 (MQ=33) tAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTAt > 1:1783888/1‑100 (MQ=32) aaCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATg < 2:487679/100‑1 (MQ=33) aaCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATg < 2:2169012/100‑1 (MQ=33) aaCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATg < 2:292574/100‑1 (MQ=17) aCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg > 1:1659605/1‑100 (MQ=32) tGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGt > 1:629187/1‑98 (MQ=32) gATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCg < 1:1556453/100‑1 (MQ=32) ccGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAt < 1:1306672/100‑1 (MQ=33) ccGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAt > 2:2033671/1‑100 (MQ=32) ccGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAt > 2:1042614/1‑100 (MQ=32) cGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAtt > 1:2130438/1‑100 (MQ=33) cGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAt > 1:729724/1‑99 (MQ=32) aaGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAg < 2:549563/100‑1 (MQ=17) aaGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAg < 2:563138/100‑1 (MQ=17) gagTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTg < 2:1428863/100‑1 (MQ=32) agTTCATATCGACGGCTGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTATGTCCCAAAGGTATGGCTGTTCGCCATTTAAAg < 2:228023/97‑1 (MQ=255) ttCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTa > 2:1500725/1‑100 (MQ=33) cATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTAcg < 2:1953058/100‑1 (MQ=18) tatCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcgcg > 1:2017371/1‑100 (MQ=33) atCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGa < 1:1969389/100‑1 (MQ=33) atCGACGGCGGTGTTTGGCACCTCGATGTCGGCACATCCCATCCCGGAGCAGAAGTAGGTCCCAAGGGAATAGCTGTTCGCCATTTAAAGTGGCAAGCGa > 1:573814/1‑100 (MQ=11) tCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAg > 1:602327/1‑100 (MQ=33) gACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCt > 1:1485730/1‑100 (MQ=33) gACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCt > 1:1048150/1‑100 (MQ=33) gACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCt < 2:1059199/100‑1 (MQ=33) gACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAg < 2:280751/98‑1 (MQ=18) ggcggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTggg < 2:1642689/100‑1 (MQ=32) ggcggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTggg < 2:2196449/100‑1 (MQ=18) gtgtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTa < 1:2124684/100‑1 (MQ=33) gtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGa < 2:1427512/100‑1 (MQ=32) gtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGa < 1:326297/100‑1 (MQ=32) gtTTGGCACCTCGATGTCGGCTAATCACATCCTGGAGCTGAAGGAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGa > 2:1372454/1‑100 (MQ=12) tttGGCACTTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAg < 2:1566766/98‑1 (MQ=17) ggCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgt > 2:506345/1‑100 (MQ=32) cACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcg < 1:1647617/100‑1 (MQ=33) cACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcg < 2:937145/100‑1 (MQ=18) ccTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTg > 1:639881/1‑100 (MQ=33) ccTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTg < 2:843199/100‑1 (MQ=17) ccTCGATGTCGGCTCATCACATCATGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTg > 2:1109435/1‑100 (MQ=17) cTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTga < 2:204179/100‑1 (MQ=32) tCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTg < 2:1543240/98‑1 (MQ=17) tGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAg < 2:2222986/100‑1 (MQ=33) gTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGt > 1:156827/1‑100 (MQ=33) gTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGt > 1:1093277/1‑100 (MQ=18) tCGGCTCATCACATACTGGAGCTGAAGTAAGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTTGGTTTAGAACGTCGTGAGACAGtt > 2:1744028/1‑100 (MQ=255) cGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTc < 2:472921/100‑1 (MQ=33) cGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTc < 2:1621191/100‑1 (MQ=33) ggCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTc < 2:1544253/99‑1 (MQ=32) ggCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCg < 2:1271431/100‑1 (MQ=18) ggCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCg < 2:781620/100‑1 (MQ=17) gCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCg < 2:558526/99‑1 (MQ=32) cTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTGCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGt > 1:1292786/1‑100 (MQ=18) catcaCATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTcc < 2:787636/100‑1 (MQ=33) tCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTg > 2:1612456/1‑100 (MQ=33) tCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTg > 1:1077250/1‑100 (MQ=32) cTGGAGCAGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGcc > 1:1177324/1‑100 (MQ=12) tGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCg > 1:154133/1‑100 (MQ=32) ggAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGt > 2:1751439/1‑100 (MQ=32) | GGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGT > NC_000913/3946148‑3946325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |