Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 12,075 (A)7→6 intergenic (‑289/‑88) yaaI ← / → dnaK UPF0412 family protein/chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091312,0690A.96.8% 399.5 / 5.5 94intergenic (‑283/‑94)yaaI/dnaKUPF0412 family protein/chaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base . (42/49);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 105 total aligned reads displayed.

GCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGG  >  NC_000913/11969‑12167
                                                                                                    |                                                                                                  
gcgAAATTTCTGCGCAAAAGCACAAAAAATTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCa                                                                                                    >  2:858628/1‑100 (MQ=255)
   aaaTTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCGTTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaa                                                                                                  <  2:3003309/100‑1 (MQ=255)
   aaaTTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaa                                                                                                  <  2:3000308/100‑1 (MQ=255)
    aaTTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaa                                                                                                 <  1:112022/100‑1 (MQ=255)
      tttCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaaaa                                                                                               >  1:275627/1‑100 (MQ=255)
      tttCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaaaa                                                                                               <  2:1545120/100‑1 (MQ=255)
       ttCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaaaat                                                                                              <  2:581336/100‑2 (MQ=255)
          tGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaaaatgaa                                                                                           <  1:260555/100‑5 (MQ=255)
             gcAAAAGCACAAAAAATTTTTGAATCTCCCCATTGATGACGTGGTTTACGACTCCATTTAGTAGTCAACCGCAGTGCGTGAGTCTGCAAAAAATGAAAtt                                                                                       >  2:2900118/1‑100 (MQ=255)
              cAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTg                                                                                      >  2:2275658/1‑100 (MQ=255)
               aaaaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCGTTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTgg                                                                                     <  1:1563665/100‑1 (MQ=255)
               aaaaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTg                                                                                      >  2:1422257/1‑99 (MQ=255)
                aaaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTggg                                                                                    <  2:918849/100‑1 (MQ=255)
                 aaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGc                                                                                   <  2:2679336/100‑1 (MQ=255)
                 aaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGc                                                                                   <  1:808432/100‑1 (MQ=255)
                 aaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGc                                                                                   <  1:2275687/100‑1 (MQ=255)
                 aaGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGc                                                                                   <  1:2096378/100‑1 (MQ=255)
                    cacaAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGt                                                                                >  1:1253545/1‑100 (MQ=255)
                     acaAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGCGTGAGTCTGCAAAAAATGAAATTGGGCAGtt                                                                               >  1:116668/1‑100 (MQ=255)
                     acaAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGtt                                                                               >  2:230767/1‑100 (MQ=255)
                      caAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTg                                                                              >  1:3041293/1‑100 (MQ=255)
                      caAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTg                                                                              <  2:296881/100‑1 (MQ=255)
                       aaaaaaTTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTg                                                                              <  2:1293491/99‑1 (MQ=255)
                       aaaaaaTTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGa                                                                             >  1:2270846/1‑100 (MQ=255)
                        aaaaaTTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGa                                                                             >  2:2190125/1‑99 (MQ=255)
                         aaaaTTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGaaa                                                                           >  2:212992/1‑100 (MQ=255)
                             tttttGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAg                                                                       >  1:2519909/1‑100 (MQ=255)
                               tttGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGAc                                                                     >  1:468642/1‑100 (MQ=255)
                                ttGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACg                                                                    <  2:104659/100‑1 (MQ=255)
                                  gCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGtt                                                                  >  2:1609022/1‑100 (MQ=255)
                                   cATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGttt                                                                 >  2:2366962/1‑100 (MQ=255)
                                   cATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGttt                                                                 >  2:1505694/1‑100 (MQ=255)
                                   cATCTCCCCCTTGATGACGTCGTTTACGACCCCATTTAGTAGTCAACCGCAGTGTGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGttt                                                                 >  2:1870269/1‑100 (MQ=255)
                                    aTCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTc                                                                <  1:2709234/100‑1 (MQ=255)
                                    aTCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTc                                                                >  2:2059975/1‑100 (MQ=255)
                                    aTCTCCCCCTTGATGACGTGGTTTAAAAAAACATTTAGTAGTCATCCGCAGTGAAAAAAAAAAAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTc                                                                <  2:468635/100‑1 (MQ=255)
                                     tctcCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCg                                                               <  1:2551812/100‑1 (MQ=255)
                                      ctcCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGc                                                              >  1:455956/1‑100 (MQ=255)
                                        cccccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGccc                                                            >  2:16050/1‑100 (MQ=255)
                                        cccccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGccc                                                            >  1:2212669/1‑100 (MQ=255)
                                         ccccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGcccc                                                           >  2:588616/1‑100 (MQ=255)
                                         ccccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGcccc                                                           <  2:3040515/100‑1 (MQ=255)
                                          cccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCt                                                          <  1:1653491/100‑1 (MQ=255)
                                           ccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTa                                                         <  1:59165/100‑1 (MQ=255)
                                           ccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTa                                                         <  1:417570/100‑1 (MQ=255)
                                           ccTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTa                                                         <  1:1419538/100‑1 (MQ=255)
                                              tgatgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTa                                                      <  1:733723/100‑1 (MQ=255)
                                              tgatgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTa                                                      <  1:1375338/100‑1 (MQ=255)
                                               gatgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTAc                                                     <  1:976462/100‑1 (MQ=255)
                                               gatgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTAc                                                     <  1:1505710/100‑1 (MQ=255)
                                               gatgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTAc                                                     <  1:737474/100‑1 (MQ=255)
                                                atgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACa                                                    <  1:858638/100‑1 (MQ=255)
                                                atgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACa                                                    <  1:1671228/100‑1 (MQ=255)
                                                atgaCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACa                                                    <  1:240923/100‑1 (MQ=255)
                                                   aCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGAc                                                 >  1:1969546/1‑100 (MQ=255)
                                                    cGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt                                                <  1:2031756/100‑1 (MQ=255)
                                                     gTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTc                                               <  2:2390161/100‑1 (MQ=255)
                                                      tGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca                                              >  1:2431977/1‑100 (MQ=255)
                                                       gtgttccGACCCCATTTAGTAGCCAACCGCAGTGAGAGAGTCGGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCCCCCCTTTTACACACTca                                              <  2:275620/93‑1 (MQ=255)
                                                        gTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca                                            >  2:1919640/1‑100 (MQ=255)
                                                        gTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca                                            >  1:2372912/1‑100 (MQ=255)
                                                          ttACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAc                                          <  1:2221462/100‑1 (MQ=255)
                                                             cGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTTAAACCACACGTTTCGCCCCTATTACAGACTCACAACcac                                       <  1:2900158/100‑1 (MQ=255)
                                                             cGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcac                                       <  2:1336213/100‑1 (MQ=255)
                                                              gACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcaca                                      <  2:3048111/100‑1 (MQ=255)
                                                              gACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcac                                       <  2:2493535/99‑1 (MQ=255)
                                                                ccccATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACatg                                    <  2:632549/100‑1 (MQ=255)
                                                                  ccATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACatgat                                  <  2:1766715/100‑1 (MQ=255)
                                                                  ccATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACatgat                                  <  1:2948754/100‑1 (MQ=255)
                                                                  ccATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACatgat                                  >  1:1992583/1‑100 (MQ=255)
                                                                       tagtagTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCg                             >  2:396184/1‑100 (MQ=255)
                                                                       tagtagTCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCg                             >  2:192475/1‑100 (MQ=255)
                                                                         gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGATGTTTCGCCCCTATTACAGACTCACAACCTCATGATGACCgta                           <  1:2066980/100‑3 (MQ=255)
                                                                         gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGaa                           >  1:893840/1‑100 (MQ=255)
                                                                          tagtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAat                          <  1:96827/100‑1 (MQ=255)
                                                                          tagtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAat                          >  2:2870882/1‑100 (MQ=255)
                                                                           agtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAata                         <  1:2060001/100‑1 (MQ=255)
                                                                           agtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAata                         <  1:1720017/100‑1 (MQ=255)
                                                                            gtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatat                        >  1:1304136/1‑100 (MQ=255)
                                                                            gtCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatat                        >  1:2919065/1‑100 (MQ=255)
                                                                             tCAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatata                       <  1:566697/100‑1 (MQ=255)
                                                                              cAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatatat                      <  2:1253533/100‑1 (MQ=255)
                                                                              cAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatatat                      >  2:1181982/1‑100 (MQ=255)
                                                                              cAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatatat                      <  1:2775590/100‑1 (MQ=255)
                                                                              cAACCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCCATTACAGACTCACAACCACATGATGACCGActagat                      >  2:1260404/1‑100 (MQ=255)
                                                                               aaCCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAAtatata                     >  1:2880115/1‑100 (MQ=255)
                                                                                aCCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAg                    >  2:664154/1‑100 (MQ=255)
                                                                                aCCGCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCACCTATTACAGACTCACAACCACATGATGACCGAATATATAg                    >  2:1483276/1‑100 (MQ=255)
                                                                                   gCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTgg                 <  2:1592775/100‑1 (MQ=255)
                                                                                   gCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTgg                 <  1:1383727/100‑1 (MQ=255)
                                                                                   gCAGTGAGTGAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTgg                 <  2:2612991/100‑1 (MQ=255)
                                                                                     agtgagtgAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGgag               <  1:851970/100‑1 (MQ=255)
                                                                                     agtgagtgAGTCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGgag               <  2:1847340/100‑1 (MQ=255)
                                                                                        gagtgagtCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACg            >  1:543709/1‑100 (MQ=255)
                                                                                           tgagtCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGttt         >  1:2930621/1‑100 (MQ=255)
                                                                                           tgagtCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGttt         <  2:429005/100‑1 (MQ=255)
                                                                                              gtCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGCATATATAGTGGAGACGTTTAGa      >  1:90121/1‑100 (MQ=255)
                                                                                               tCTGCAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGAt     <  2:2270817/100‑1 (MQ=255)
                                                                                                 tGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATg    <  1:2639669/100‑1 (MQ=255)
                                                                                                   cAAAAAAATGAAATTGGGCAGTTGAAACAAGACGTTTCGCCCCTATTACAGACTCACAACCCAATGATGACCAAATATATAGTGGAGACgaacacctggg  >  1:1780233/1‑90 (MQ=255)
                                                                                                    |                                                                                                  
GCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGG  >  NC_000913/11969‑12167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: