BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,728,118	(C)_8→7	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,111	0	C	.	97.1%	442.4 / 8.2	103	coding (1261/1455 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base . (45/54); total (48/55)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 119 total aligned reads displayed.

ACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACAC  >  NC_000913/3728012‑3728206

 cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcc                                                                                                <  1:1643754/100‑1 (MQ=255)

 cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcc                                                                                                >  1:1558473/1‑100 (MQ=255)

 cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcc                                                                                                <  2:848272/100‑1 (MQ=255)

  tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                               <  1:644193/100‑1 (MQ=255)

   gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATcccc                                                                                              >  2:931806/1‑100 (MQ=255)

   gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccag                                                                                              >  2:1643735/1‑98 (MQ=255)

    ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccc                                                                                             <  2:1783475/100‑1 (MQ=255)

     tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                            <  2:1660476/100‑1 (MQ=255)

      cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                           >  1:1313530/1‑100 (MQ=255)

      cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                           >  2:2542916/1‑100 (MQ=255)

       tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccccg                                                                                          <  2:1911718/100‑2 (MQ=255)

          tcttAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccccgtac                                                                                       <  2:1429177/97‑5 (MQ=255)

            gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACgg                                                                                    <  1:437179/100‑1 (MQ=255)

            gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACgg                                                                                    <  1:2136614/100‑1 (MQ=255)

             tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccccgtacaga                                                                                    >  1:1429190/1‑93 (MQ=255)

             tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGt                                                                                   <  2:565221/100‑1 (MQ=255)

              aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTa                                                                                  >  2:1918347/1‑100 (MQ=255)

               gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACCGTaa                                                                                 >  1:2810866/1‑100 (MQ=255)

                 tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATc                                                                               >  1:627535/1‑100 (MQ=255)

                 tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATc                                                                               <  1:2301834/100‑1 (MQ=255)

                   ctgCAACAATTCAATGAGCGATTTCTCTGGATTAGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGTTGGCCCCACAT‑CCCCCCCGTACGGTAATCGa                                                                             <  2:1509254/98‑1 (MQ=255)

                   cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGa                                                                             <  1:513971/100‑1 (MQ=255)

                   cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGa                                                                             >  2:599918/1‑100 (MQ=255)

                    ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAg                                                                            >  2:1642564/1‑100 (MQ=255)

                     gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgc                                                                           <  1:1424913/100‑1 (MQ=255)

                      caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgct                                                                          >  1:1840406/1‑100 (MQ=255)

                      caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgct                                                                          <  1:1971193/100‑1 (MQ=255)

                      caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgc                                                                           >  2:2152965/1‑99 (MQ=255)

                       aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgctg                                                                         <  1:209541/100‑1 (MQ=255)

                       aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgctg                                                                         <  1:209617/100‑1 (MQ=255)

                       aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgctg                                                                         <  2:149252/100‑1 (MQ=255)

                        acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgctgc                                                                        <  1:1794448/100‑1 (MQ=255)

                         caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgctgct                                                                       <  2:1351073/100‑1 (MQ=255)

                         caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgctgct                                                                       >  1:1078518/1‑100 (MQ=255)

                         caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAgatgc                                                                        >  2:914309/1‑99 (MQ=255)

                            ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGAc                                                                    <  1:1922998/100‑1 (MQ=255)

                              cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                  <  1:2861825/100‑1 (MQ=255)

                               aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                 <  2:2328535/100‑1 (MQ=255)

                                aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGAcccc                                                                 >  2:680844/1‑97 (MQ=255)

                                 tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCAGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                               <  2:1955249/100‑1 (MQ=255)

                                  gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                <  2:585212/98‑1 (MQ=255)

                                  gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                              <  2:2640979/100‑1 (MQ=255)

                                     cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                           >  2:1341187/1‑100 (MQ=255)

                                      gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                          >  2:2804701/1‑100 (MQ=255)

                                      gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTCGCTGCGCCCAGCGCTGGACCCACAT‑CCCCCACGTACGGTAATCGAGCTGCTGACCGCtcaca                                                            >  1:2572789/1‑94 (MQ=255)

                                       aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                         >  1:1418114/1‑100 (MQ=255)

                                       aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                         <  1:2963021/100‑1 (MQ=255)

                                       aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                         <  1:1501790/100‑1 (MQ=255)

                                       aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                         <  2:40825/100‑1 (MQ=255)

                                          tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                      <  1:1050137/100‑1 (MQ=255)

                                          tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                      <  2:1243483/100‑1 (MQ=255)

                                          tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTAACCGCTGATATCCGcc                                                      >  2:2016961/1‑100 (MQ=255)

                                           ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                      >  2:2391963/1‑99 (MQ=255)

                                               ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                                  >  1:1524680/1‑99 (MQ=255)

                                               ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                 >  2:3050702/1‑100 (MQ=255)

                                                gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                <  1:71583/100‑1 (MQ=255)

                                                    cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATACGCCAGCATCTGa                                             >  2:2576358/1‑99 (MQ=255)

                                                    cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                            <  1:406847/100‑1 (MQ=255)

                                                      ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                          >  2:2097442/1‑100 (MQ=255)

                                                       cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                         <  1:1451632/100‑1 (MQ=255)

                                                       cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                         <  1:1779969/100‑1 (MQ=255)

                                                       cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                         <  1:2824942/100‑1 (MQ=255)

                                                        gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                        <  1:2968407/100‑1 (MQ=255)

                                                         cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                       <  2:1650496/100‑1 (MQ=255)

                                                         cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                       <  1:701234/100‑1 (MQ=255)

                                                            tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                    <  2:1508653/100‑1 (MQ=255)

                                                              tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTc                                  >  1:984418/1‑100 (MQ=255)

                                                              tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                  <  1:599926/100‑1 (MQ=255)

                                                               gcgcCAGCATTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGTGCTGCTGACAGCTCATATACCCCCCCATCTGACGc                                          >  2:2612332/1‑91 (MQ=255)

                                                                cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                >  1:1678432/1‑100 (MQ=255)

                                                                 gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                               <  1:608624/100‑1 (MQ=255)

                                                                 gcCAGCCTTGCTGCGCCCAGTGCTGGCCACACA‑ACCCCCCCGTACGGTACTCGCGCTGCTGCCCGATGCTCTCCACCAGCATCTGACGCCAGTACTCGCt                               >  2:2471102/1‑100 (MQ=255)

                                                                    aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCGCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCTGCCAGCATCTGACGCCAGTACTCACTAcg                            >  2:1837097/1‑100 (MQ=255)

                                                                         tgctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                       >  1:915752/1‑100 (MQ=255)

                                                                          gctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                      <  2:534092/100‑1 (MQ=255)

                                                                           ctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACTGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTTACGCCAGTACTCACTACGcgccccg                     <  2:1984189/100‑1 (MQ=255)

                                                                            tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc                    >  2:813966/1‑100 (MQ=255)

                                                                             gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                   >  2:2917346/1‑100 (MQ=255)

                                                                              cgcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                  <  1:1188112/100‑1 (MQ=255)

                                                                               gcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                 >  1:1058722/1‑100 (MQ=255)

                                                                               gcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                 <  2:2286560/100‑1 (MQ=255)

                                                                               gcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                 <  1:1341200/100‑1 (MQ=255)

                                                                               gcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                 >  1:709585/1‑100 (MQ=255)

                                                                               gcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                 >  1:800361/1‑100 (MQ=255)

                                                                               gcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCCGATCTCCGCCAGCATCTGACGCCAGTACTCACTCCGCgccccgcccc                 >  1:1438143/1‑100 (MQ=255)

                                                                                cccAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                >  2:1565197/1‑100 (MQ=255)

                                                                                cccAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                <  1:2827969/100‑1 (MQ=255)

                                                                                   aGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt             <  1:304730/100‑1 (MQ=255)

                                                                                    gTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc            <  2:1673436/100‑1 (MQ=255)

                                                                                      gCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGAGCGATGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCaa          >  1:748188/1‑100 (MQ=255)

                                                                                        tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGGCAGTACTCACTACGCGCCCCGCCCCCAATCAAc         >  2:826226/1‑100 (MQ=255)

                                                                                        tGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg        <  1:996932/100‑1 (MQ=255)

                                                                                         ggCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt       >  2:1111150/1‑100 (MQ=255)

                                                                                           ccccACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa     >  1:596085/1‑100 (MQ=255)

                                                                                           ccccACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa     >  1:1948369/1‑100 (MQ=255)

                                                                                           ccccACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCCGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTaa     >  2:1392734/1‑100 (MQ=255)

                                                                                            cccACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac    <  2:1313518/100‑1 (MQ=255)

                                                                                            cccACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac    >  2:2607513/1‑100 (MQ=255)

                                                                                             ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac    <  2:344577/100‑1 (MQ=255)

                                                                                              cacaT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacgc  >  1:1578395/1‑98 (MQ=255)

ACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACAC  >  NC_000913/3728012‑3728206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`