Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 632,017 (G)7→6 intergenic (+18/‑165) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913632,0110G.100.0% 522.5 / NA 114intergenic (+12/‑171)cstA/ybdDcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (49/65);  total (49/65)

Only 100 of 127 total aligned reads displayed.

TCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTG  >  NC_000913/631912‑632105
                                                                                                   |                                                                                              
tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAg                                                                                                >  1:1003922/1‑100 (MQ=255)
tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAg                                                                                                >  2:2539957/1‑100 (MQ=255)
tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAg                                                                                                >  1:2908794/1‑100 (MQ=255)
tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGCGAg                                                                                                >  2:2254568/1‑100 (MQ=255)
 ccGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgg                                                                                               >  2:689554/1‑100 (MQ=255)
  cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggg                                                                                              <  1:1510874/100‑1 (MQ=255)
      gCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggggga                                                                                          <  1:2188886/100‑2 (MQ=255)
      gCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggggga                                                                                          <  2:2667031/100‑2 (MQ=255)
       ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggggat                                                                                         >  2:2616504/1‑98 (MQ=255)
        cAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggggatg                                                                                        >  1:2202744/1‑97 (MQ=255)
        cAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggggatg                                                                                        <  1:1675099/100‑4 (MQ=255)
           cGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTg                                                                                    >  2:86547/1‑100 (MQ=255)
            ggCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTgg                                                                                   >  1:2680609/1‑100 (MQ=255)
            ggCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTgg                                                                                   <  2:1931781/100‑1 (MQ=255)
            ggCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTgg                                                                                   <  1:1821174/100‑1 (MQ=255)
            ggCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTgg                                                                                   >  1:505475/1‑100 (MQ=255)
              cGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCg                                                                                 >  1:2840653/1‑100 (MQ=255)
              cGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCg                                                                                 <  1:1432444/100‑1 (MQ=255)
                aaagaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGaa                                                                               >  2:105200/1‑100 (MQ=255)
                  agaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCGCACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATg                                                                             <  1:696309/100‑1 (MQ=255)
                   gaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGt                                                                            >  2:351393/1‑100 (MQ=255)
                    tttcGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGtt                                                                           <  2:1292569/97‑1 (MQ=255)
                    aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGtt                                                                           >  1:1950065/1‑100 (MQ=255)
                    aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGtt                                                                           <  2:1563185/100‑1 (MQ=255)
                    aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGtt                                                                           >  2:2191043/1‑100 (MQ=255)
                    aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGtt                                                                           >  1:393411/1‑100 (MQ=255)
                      aCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTgg                                                                         <  2:510061/100‑1 (MQ=255)
                        gCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCt                                                                       <  2:308616/100‑1 (MQ=255)
                         ccGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCtt                                                                      >  2:86642/1‑100 (MQ=255)
                           gTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAg                                                                    <  2:2959496/100‑1 (MQ=255)
                           gTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAg                                                                    <  2:1795721/100‑1 (MQ=255)
                              tGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGc                                                                 >  1:379564/1‑100 (MQ=255)
                               gAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGcc                                                                >  2:2410012/1‑100 (MQ=255)
                                aaCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGccc                                                               >  2:227791/1‑100 (MQ=255)
                                aaCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGccc                                                               <  1:1433901/100‑1 (MQ=255)
                                 aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                                                              <  2:278273/100‑1 (MQ=255)
                                    aaTGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGTATGTTGGCGAATGTTGGCTTAGTGCCCAggg                                                           >  1:1668662/1‑100 (MQ=255)
                                     aTGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                                                          <  1:86551/100‑1 (MQ=255)
                                     aTGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                                                          <  2:1607158/100‑1 (MQ=255)
                                     aTGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                                                          <  1:1915564/100‑1 (MQ=255)
                                       gCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTc                                                        >  1:2820092/1‑100 (MQ=255)
                                       gCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGCGccaaaggctc                                                        >  1:2854308/1‑92 (MQ=255)
                                        ccGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                       >  1:2627844/1‑100 (MQ=255)
                                         cGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAGGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTccc                                                      <  1:2847180/100‑1 (MQ=255)
                                         cGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTccc                                                      <  1:497344/100‑1 (MQ=255)
                                         cGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTccc                                                      <  1:2122438/100‑1 (MQ=255)
                                           gAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc                                                    <  2:2161134/100‑1 (MQ=255)
                                           gAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc                                                    <  2:281391/100‑1 (MQ=255)
                                           gAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc                                                    <  1:2505484/100‑1 (MQ=255)
                                            aaaaTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                   <  2:2224821/100‑1 (MQ=255)
                                            aaaaTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                   <  1:1294638/100‑1 (MQ=255)
                                              aaTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCa                                                 <  2:2166240/100‑1 (MQ=255)
                                               aTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                <  2:2659456/100‑1 (MQ=255)
                                               aTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                <  2:2300663/100‑1 (MQ=255)
                                                 gTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAccc                                              >  2:585242/1‑100 (MQ=255)
                                                 gTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAccc                                              <  1:2616541/100‑1 (MQ=255)
                                                    gaggagATCGTGGCGCAGTCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTaa                                           <  1:1324415/100‑1 (MQ=255)
                                                      ggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                         <  1:255435/100‑1 (MQ=255)
                                                      ggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                         <  1:2018747/100‑1 (MQ=255)
                                                      ggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                         <  2:2156698/100‑1 (MQ=255)
                                                      ggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                         >  1:3034575/1‑100 (MQ=255)
                                                       gagaTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAccc                                        >  2:1547548/1‑100 (MQ=255)
                                                          aTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctct                                     >  1:1937391/1‑100 (MQ=255)
                                                          aTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctct                                     <  2:2245859/100‑1 (MQ=255)
                                                            cGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTcc                                   >  2:2246635/1‑100 (MQ=255)
                                                               ggCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCg                                <  2:2385279/100‑1 (MQ=255)
                                                               ggCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCg                                <  2:2202715/100‑1 (MQ=255)
                                                                 cgcAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGt                              >  2:543190/1‑100 (MQ=255)
                                                                   cAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                            >  1:162671/1‑100 (MQ=255)
                                                                   cAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                            <  1:585250/100‑1 (MQ=255)
                                                                   cAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                            <  2:2939154/100‑1 (MQ=255)
                                                                   cAGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                            <  1:952727/100‑1 (MQ=255)
                                                                    aGGCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTggg                           <  1:996150/100‑1 (MQ=255)
                                                                     ggCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgggg                          >  2:2325945/1‑100 (MQ=255)
                                                                     ggCAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgggg                          >  1:1495761/1‑100 (MQ=255)
                                                                       cAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                        >  1:1669762/1‑100 (MQ=255)
                                                                       cAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                        <  2:1623225/100‑1 (MQ=255)
                                                                       cAAAAGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCg                        <  2:2818238/100‑1 (MQ=255)
                                                                        aaaaGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGa                       <  1:2492992/100‑1 (MQ=255)
                                                                          aaGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgg                     <  2:899765/100‑1 (MQ=255)
                                                                           aGGCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                    >  2:423197/1‑100 (MQ=255)
                                                                            ggCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                   <  1:94879/100‑1 (MQ=255)
                                                                            ggCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                   <  2:201108/100‑1 (MQ=255)
                                                                            ggCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                   <  1:94567/100‑1 (MQ=255)
                                                                            ggCGCACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                   <  1:2410044/100‑1 (MQ=255)
                                                                              cgcACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGAc                 <  2:1172279/100‑1 (MQ=255)
                                                                               gcACACTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACt                >  1:2949611/1‑100 (MQ=255)
                                                                                  cacTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAc             <  1:2081115/100‑1 (MQ=255)
                                                                                   acTAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAcc            <  1:1840480/100‑1 (MQ=255)
                                                                                    ntAAAGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCg           >  2:680218/2‑100 (MQ=255)
                                                                                      aaaGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAg         <  1:2958570/100‑1 (MQ=255)
                                                                                      aaaGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAg         <  1:1748848/100‑1 (MQ=255)
                                                                                        aGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgcg       >  2:1168217/1‑100 (MQ=255)
                                                                                        aGTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgcg       >  2:723448/1‑100 (MQ=255)
                                                                                         gTCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgcgc      >  2:1028430/1‑100 (MQ=255)
                                                                                          tCAGAGTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACACTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg     >  2:304785/1‑100 (MQ=255)
                                                                                            agagTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt   <  2:452638/100‑1 (MQ=255)
                                                                                            agagTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt   >  1:363637/1‑100 (MQ=255)
                                                                                            agagTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt   <  1:11807/100‑1 (MQ=255)
                                                                                             gagTGA‑GGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTg  >  2:1166510/1‑100 (MQ=255)
                                                                                                   |                                                                                              
TCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTG  >  NC_000913/631912‑632105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑