BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,181	(T)_5→6	coding (516/951 nt)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,176	1	.	T	98.0%	337.3 / ‑0.6	99	coding (511/951 nt)	talA	transaldolase A
Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (48/49); total (48/51)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 114 total aligned reads displayed.

GGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑T‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGT  >  NC_000913/2579077‑2579270

ggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCg                                                                                                   <  2:1249006/100‑1 (MQ=255)

ggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCg                                                                                                   >  2:275093/1‑100 (MQ=255)

 gaaTTCGCTCGGCAGAAGAACAGAAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCgn                                                                                                  <  2:2646520/100‑2 (MQ=255)

      cgcgcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑tt                                                                                          >  2:1309922/1‑99 (MQ=255)

      cgcgcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑tt                                                                                          <  1:2940465/100‑2 (MQ=255)

        cgcgGCAGAAGAGCTGGAAAAAGAAGGTATTTACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttct                                                                                        <  1:3050629/100‑4 (MQ=255)

        cgcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttct                                                                                        <  1:607362/100‑4 (MQ=255)

         gcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttctg                                                                                       <  1:2485817/100‑5 (MQ=255)

         gcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttctg                                                                                       <  1:1092403/100‑5 (MQ=255)

         gcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttctg                                                                                       <  1:110571/100‑5 (MQ=255)

         gcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttctg                                                                                       >  1:1470695/1‑96 (MQ=255)

          cgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttctga                                                                                      <  2:669623/100‑6 (MQ=255)

          cgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑t‑ttt‑ttctca                                                                                      <  1:2074215/100‑6 (MQ=255)

           ggCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGAt                                                                                      <  1:886140/100‑1 (MQ=255)

           ggCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGAt                                                                                      <  2:1632193/100‑1 (MQ=255)

            gCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGAtt                                                                                     <  1:686982/100‑1 (MQ=255)

              agaagaGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTc                                                                                   >  2:922896/1‑100 (MQ=255)

                aagaGCTGGACAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGGCTCTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCgt                                                                                 <  2:2007104/100‑2 (MQ=255)

                aagaGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGc                                                                                 <  1:2211885/100‑1 (MQ=255)

                  gagCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCg                                                                               <  1:3033994/100‑1 (MQ=255)

                  gagCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCg                                                                               <  1:1913648/100‑1 (MQ=255)

                   agCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGt                                                                              <  1:1644570/100‑1 (MQ=255)

                   agCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGt                                                                              <  2:689391/100‑1 (MQ=255)

                   agCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGt                                                                              >  2:2454386/1‑100 (MQ=255)

                    gCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGtt                                                                             <  2:2175035/100‑1 (MQ=255)

                     cTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGttt                                                                            <  1:1495654/100‑1 (MQ=255)

                     cTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGttt                                                                            >  2:1469905/1‑100 (MQ=255)

                      tGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTg                                                                           >  2:1540469/1‑100 (MQ=255)

                      tGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTg                                                                           <  1:49451/100‑1 (MQ=255)

                         aaaaaGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCg                                                                        <  1:992261/100‑1 (MQ=255)

                         aaaaaGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCg                                                                        <  1:949538/100‑1 (MQ=255)

                          aaaaGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCgg                                                                       <  1:1469918/100‑1 (MQ=255)

                           aaaGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCggg                                                                      <  2:1125674/100‑1 (MQ=255)

                             agaagGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGGAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCg                                                                    <  1:1010953/100‑1 (MQ=255)

                               aagGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTa                                                                  <  2:835743/100‑1 (MQ=255)

                                   tATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTa                                                              <  1:2764866/100‑1 (MQ=255)

                                   tATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTa                                                              >  2:1714211/1‑100 (MQ=255)

                                   tATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTa                                                              >  1:46884/1‑100 (MQ=255)

                                    aTTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTAt                                                             <  2:2728373/100‑1 (MQ=255)

                                    aTTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTAt                                                             >  2:400864/1‑100 (MQ=255)

                                    aTTAACTGCAACCGGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTAt                                                             <  1:179217/100‑1 (MQ=255)

                                       aaCTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTAttt                                                               >  2:2938998/1‑95 (MQ=255)

                                       aaCTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGAc                                                          >  1:145621/1‑100 (MQ=255)

                                       aaCTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGAGCGTATTTATGAc                                                          >  1:1027627/1‑100 (MQ=255)

                                         cTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑TATTT‑TCTGATTTCGCCGTTTGTCGGTCGTATTTATGACt                                                         <  2:1888764/99‑1 (MQ=255)

                                         cTGCAACCTGACGCTGCTGTTATCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TATGATTTCGCCGTTTGTCGGGCGTATTTATGACTg                                                        >  2:1763436/1‑100 (MQ=255)

                                          tGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTgg                                                       <  1:100758/100‑1 (MQ=255)

                                          tGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTgg                                                       <  1:102212/100‑1 (MQ=255)

                                              aCCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATc                                                   >  1:1568303/1‑100 (MQ=255)

                                              aCCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATc                                                   <  2:2283514/100‑1 (MQ=255)

                                                 tGACGCTGCTGTTTTCTTTTTAACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAg                                                 <  2:1963202/99‑1 (MQ=255)

                                                 tGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAgg                                                <  1:400871/100‑1 (MQ=255)

                                                 tGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAgg                                                >  1:493399/1‑100 (MQ=255)

                                                 tGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAgg                                                <  2:2415198/100‑1 (MQ=255)

                                                  gACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑T‑TTTGTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGc                                               <  2:161796/100‑1 (MQ=255)

                                                   aCGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCa                                              >  1:3020977/1‑100 (MQ=255)

                                                    cGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCAc                                             >  2:1794901/1‑100 (MQ=255)

                                                    cGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCAc                                             >  2:3033503/1‑100 (MQ=255)

                                                     gctgctGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACg                                            <  1:2202883/100‑1 (MQ=255)

                                                       tgctgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCa                                          <  2:1583246/100‑1 (MQ=255)

                                                       atctgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCa                                          <  1:275667/98‑1 (MQ=255)

                                                        ttccgatctttttGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCaa                                         <  2:2501169/91‑1 (MQ=255)

                                                        gctgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGcta                                         <  1:2827116/100‑3 (MQ=255)

                                                         ctgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAg                                        <  2:196158/100‑1 (MQ=255)

                                                          tgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGc                                       >  1:984231/1‑100 (MQ=255)

                                                          tgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGc                                       <  2:2128129/100‑1 (MQ=255)

                                                           gTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAgat                                      >  2:275660/1‑98 (MQ=255)

                                                           gTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGcc                                      <  1:638625/100‑1 (MQ=255)

                                                             tttCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGc                                       >  2:1850540/1‑97 (MQ=255)

                                                              ttCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGa                                    >  2:602831/1‑99 (MQ=255)

                                                              ttCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGAt                                   <  1:2754519/100‑1 (MQ=255)

                                                                cttccgCACAGGCACGTGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATgg                                 >  1:2049853/6‑100 (MQ=255)

                                                                cTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCCGGCATGCCAGCCGATgg                                 >  2:2127990/1‑100 (MQ=255)

                                                                cTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATgg                                 >  1:1192775/1‑100 (MQ=255)

                                                                cTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATgg                                 >  1:2308571/1‑100 (MQ=255)

                                                                cTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATgg                                 >  1:2489703/1‑100 (MQ=255)

                                                                 ttttGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCaaagatcggaa                                >  1:2501203/1‑91 (MQ=255)

                                                                  tccgCACAGGCCCGTGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAc                               >  2:1033527/4‑100 (MQ=255)

                                                                    tGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TTTTATTTTTCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAccc                             >  2:941555/1‑100 (MQ=255)

                                                                    tGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAccc                             >  1:2822344/1‑100 (MQ=255)

                                                                    tGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAccc                             >  2:1157682/1‑100 (MQ=255)

                                                                    cgCACAGGCCCGTGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAccc                             >  1:2272634/2‑100 (MQ=255)

                                                                    cgCACAGGCCCGTGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAccc                             >  1:1529782/2‑100 (MQ=255)

                                                                     gCACAGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCg                            >  1:2310646/1‑100 (MQ=255)

                                                                      nacaGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGt                           >  1:1342450/2‑100 (MQ=255)

                                                                       acaGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTa                          <  2:2175369/100‑1 (MQ=255)

                                                                        caGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTAt                         >  1:751745/1‑100 (MQ=255)

                                                                        caGGCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTAt                         >  1:1750785/1‑100 (MQ=255)

                                                                          ggCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTAtgt                       >  1:468806/1‑100 (MQ=255)

                                                                          ggCACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTAtgt                       <  2:1105299/100‑1 (MQ=255)

                                                                            cACGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATgtgg                     >  1:281483/1‑100 (MQ=255)

                                                                             aCGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATgtggt                    <  2:1980597/100‑1 (MQ=255)

                                                                              cGGGCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATgtggtg                   <  1:1893777/100‑1 (MQ=255)

                                                                               gggCCTGTGCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGtggtgg                  <  2:1224218/100‑1 (MQ=255)

                                                                                     gtgCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGaagaag            >  1:2569790/1‑100 (MQ=255)

                                                                                       gCGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGAt          >  1:1525642/1‑100 (MQ=255)

                                                                                        cGGAAGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATc         >  2:1805468/1‑100 (MQ=255)

                                                                                           aaGCAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCgg      <  1:1157694/100‑1 (MQ=255)

                                                                                              cAGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCg   >  1:481706/1‑100 (MQ=255)

                                                                                               aGGCGTT‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGt  <  1:1714231/100‑1 (MQ=255)

GGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCG‑T‑TTT‑TCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGT  >  NC_000913/2579077‑2579270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`