BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	91.8%	356.0 / 25.8	110	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (4/5); new base C (49/52); total (53/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 123 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCA  >  NC_000913/3379160‑3379354

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGt‑tt                                                                                                     >  1:1412983/1‑100 (MQ=255)

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑c                                                                                                   >  1:1127151/1‑100 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc                                                                                               >  1:2979416/1‑100 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑c                                                                                           >  1:1111274/1‑100 (MQ=255)

       cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑cc                                                                                          <  2:2381683/100‑1 (MQ=255)

        gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CC‑C‑CCg                                                                                         <  1:2353430/100‑1 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑cccgcc                                                                                      <  2:1494199/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑cccgcc                                                                                      >  1:2836236/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑cccgcc                                                                                      <  1:893263/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑cccgcc                                                                                      <  1:894517/100‑5 (MQ=255)

            taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑ccccgcc                                                                                     >  1:1151552/1‑95 (MQ=255)

              aaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑cc‑c‑cccccc                                                                                      >  2:3024354/1‑96 (MQ=255)

               aaGAGACCGCCATCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAg                                                                                     <  1:1798748/98‑1 (MQ=255)

               aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAgcg                                                                                   <  2:357378/100‑1 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CC‑C‑CCGCCAGCGcc                                                                                 <  1:2642001/100‑1 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTACCGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCaa                                                                               >  2:2620547/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCaa                                                                               >  1:2267545/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCaa                                                                               <  1:1872456/100‑1 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCaa                                                                               <  2:2499113/100‑1 (MQ=255)

                     ccGCCAGCGTGTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGCATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAAcc                                                                             <  2:2004780/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAAcc                                                                             <  1:1553914/100‑1 (MQ=255)

                      cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCa                                                                            <  2:419471/100‑1 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCa                                                                            >  2:1324760/1‑99 (MQ=255)

                         cAGCGTCTGGTTCTTGACGCCCATTGTTGCTGATGGCCGATGTTTGATTCCTAACGTCAGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATaa                                                                         <  2:1251528/100‑2 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAg                                                                         <  1:2087133/100‑1 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCGTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGt                                                                        >  2:19648/1‑100 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGt                                                                        <  1:1185884/100‑1 (MQ=255)

                            cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGttt                                                                      >  1:1450187/1‑100 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTa                                                                     >  2:1147092/1‑100 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATcc                                                                  >  1:2247421/1‑100 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATcc                                                                  <  2:1006409/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCa                                                                 >  1:2873683/1‑100 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCa                                                                 <  2:1512815/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCa                                                                 <  2:2916212/100‑1 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCAt                                                                >  1:1315774/1‑100 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCAtt                                                               >  1:427410/1‑100 (MQ=255)

                                    tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCAttt                                                              >  1:1033724/1‑100 (MQ=255)

                                    tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CC‑C‑CCACCAGCGCCAACCATAGTTTATCCATTTg                                                             <  1:2889751/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTg                                                             <  1:1338323/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTg                                                             <  1:1270527/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTg                                                             <  2:135323/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTg                                                             <  1:415096/100‑1 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                        >  1:157891/1‑100 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                     <  2:1252177/100‑1 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                    >  1:9578/1‑100 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                    >  2:1452843/1‑100 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CC‑CACCGCCAGCGGCAACCATAGTTTATCCATTTGCGCCCGGGt                                                    >  2:764461/1‑100 (MQ=255)

                                                ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                  >  2:2705351/1‑100 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                 >  2:257578/1‑100 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                 >  1:1877724/1‑100 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                                <  2:658118/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATca                                             <  2:2673480/100‑2 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                             <  2:2301320/100‑1 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGATGAATTTTCTCGT‑TT‑CC‑CCAC‑CCGCCAGAGCCAAACATAGTTTATCCATTTGCGACCAGGCTTCAt                                               >  2:1389758/1‑97 (MQ=255)

                                                       tAATGGCCGATTGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                           <  1:620507/100‑1 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                           <  2:2700233/100‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                          >  2:3006446/1‑100 (MQ=255)

                                                         ttGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                          <  2:2590505/98‑1 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                        >  1:2590542/1‑100 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                       <  1:14322/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                      <  1:675488/100‑1 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                    >  2:2373973/1‑100 (MQ=255)

                                                               gATGGTTGATTTCTAGCGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                   <  1:3006487/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                              <  1:360914/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                              >  2:1637210/1‑100 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTTAAGTGGCGAAAGCca                            >  2:786425/1‑100 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                            <  1:297126/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATTCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                          <  2:1673877/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                          <  2:313619/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                          >  1:1555825/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                          <  1:706476/100‑1 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                         >  2:1651583/1‑100 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                        >  2:140404/1‑100 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                        >  1:535766/1‑100 (MQ=255)

                                                                           cTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                       >  2:3012667/1‑100 (MQ=255)

                                                                             aaCGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                     >  1:1435215/1‑100 (MQ=255)

                                                                              aCGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                    <  2:768923/100‑1 (MQ=255)

                                                                               cGTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCAAAGATgcgc                   >  2:196888/1‑100 (MQ=255)

                                                                                gTCGGTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                  <  2:407406/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga               >  2:1389437/1‑100 (MQ=255)

                                                                                   ggTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga               >  1:2129534/1‑100 (MQ=255)

                                                                                   ggTGAATTTTCTCGT‑TT‑CCACC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCTCGGGTTTCATGGTGAAGTGGCGAAAGCCACAGATGCGCCtga               >  1:1134986/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag              >  2:2114351/1‑100 (MQ=255)

                                                                                      gAATTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg            <  1:2374003/100‑1 (MQ=255)

                                                                                        nttttCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag          >  2:1725816/2‑100 (MQ=255)

                                                                                        aTTTTCTCGTCTC‑CC‑CC‑C‑CCGCCAGCGACACCAATAGTTTATACACTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag          <  1:1668318/100‑1 (MQ=255)

                                                                                        aTTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag          >  1:1041401/1‑100 (MQ=255)

                                                                                        aTTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag          <  1:573520/100‑1 (MQ=255)

                                                                                        aTTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag          <  2:2267516/100‑1 (MQ=255)

                                                                                        aTTTTCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga           >  1:1575256/1‑99 (MQ=255)

                                                                                         ttttCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt         >  2:785590/1‑100 (MQ=255)

                                                                                          tttCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg        <  1:2257390/100‑1 (MQ=255)

                                                                                           ttCTCGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt       >  1:2220636/1‑100 (MQ=255)

                                                                                            tctcGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa      <  1:790786/100‑1 (MQ=255)

                                                                                            tctcGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa      >  2:2261239/1‑100 (MQ=255)

                                                                                             ctcGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc     >  1:2515653/1‑100 (MQ=255)

                                                                                             ctcGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc     >  1:364320/1‑100 (MQ=255)

                                                                                               cGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc   >  1:2878415/1‑100 (MQ=255)

                                                                                               cGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc   >  2:2427555/1‑100 (MQ=255)

                                                                                               cGT‑TTCCC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc   <  2:1408659/100‑1 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CC‑C‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCA  >  NC_000913/3379160‑3379354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`