Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,525,826 (A)6→7 coding (324/561 nt) nudE ← adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,525,8201.A91.2% 366.7 / 26.7 114coding (330/561 nt)nudEadenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase
Reads supporting (aligned to +/- strand):  ref base . (6/4);  new base A (52/52);  total (58/56)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

CTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTT  >  NC_000913/3525721‑3525914
                                                                                                      |                                                                                                 
ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCttcttc                                                                                                    >  2:1808205/1‑100 (MQ=255)
ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCttcttc                                                                                                    >  1:767928/1‑100 (MQ=255)
 tctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCa                                                                                                  <  1:2632180/100‑1 (MQ=255)
 tctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCa                                                                                                  >  1:1363692/1‑100 (MQ=255)
 tctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCa                                                                                                  <  1:1633354/100‑1 (MQ=255)
  ctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaa                                                                                                 >  2:2457816/1‑100 (MQ=255)
  ctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaa                                                                                                 >  2:612594/1‑100 (MQ=255)
   tGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaa                                                                                                >  1:581964/1‑100 (MQ=255)
    ggCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaaa                                                                                              >  2:1142186/1‑100 (MQ=255)
      ntCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGGTATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCCTCTTCaaaaaa                                                                                           >  1:1137460/2‑100 (MQ=255)
      cTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaaaaa                                                                                           >  2:2293059/1‑100 (MQ=255)
      cTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCGGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaaaaa                                                                                           >  2:561103/1‑100 (MQ=255)
        cATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaaaaaag                                                                                         <  2:1226705/100‑3 (MQ=255)
         aTCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaaaaaagt                                                                                        >  2:514021/1‑97 (MQ=255)
          tCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCaaaaaaagtc                                                                                       >  2:1665789/1‑96 (MQ=255)
             ccTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGa                                                                                    >  1:1159801/1‑100 (MQ=255)
              cTTCCAGTGATTCCGGGTAGAGATCTTGCGATAACACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAGAAAGTCAGAt                                                                                   >  2:2008406/1‑100 (MQ=255)
                 ccAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGt                                                                                >  2:1343815/1‑100 (MQ=255)
                   aGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTc                                                                              >  1:684618/1‑100 (MQ=255)
                   aGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTc                                                                              <  2:2678342/100‑1 (MQ=255)
                    gTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCg                                                                             >  2:1512546/1‑100 (MQ=255)
                       aTTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTc                                                                          <  2:2661206/100‑1 (MQ=255)
                       aTTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTc                                                                          >  1:759551/1‑100 (MQ=255)
                        ttCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTcc                                                                         >  1:2755313/1‑100 (MQ=255)
                          ccGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCaa                                                                       >  1:1349077/1‑100 (MQ=255)
                            gggTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAAt                                                                     <  1:2378662/100‑1 (MQ=255)
                              gTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATcc                                                                   <  1:1215295/100‑1 (MQ=255)
                               tAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCa                                                                  >  2:592610/1‑100 (MQ=255)
                                agagATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTTAAAAAAAGTCAGATCGTTCGCTCCAAATCCaa                                                                 <  1:2898176/100‑1 (MQ=255)
                                agagATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAAc                                                                >  1:2745540/1‑100 (MQ=255)
                                 gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAAc                                                                >  2:1992188/1‑100 (MQ=255)
                                 gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAAc                                                                <  1:1969538/100‑1 (MQ=255)
                                 gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAAc                                                                >  1:1577330/1‑100 (MQ=255)
                                  agagCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAAcc                                                               <  2:32735/100‑1 (MQ=255)
                                  agaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAAcc                                                               <  1:2046555/100‑1 (MQ=255)
                                  agaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAAcc                                                               >  1:2889291/1‑100 (MQ=255)
                                        tGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGATGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCtcttct                                                         <  2:1663217/100‑1 (MQ=255)
                                        tGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCtcttct                                                         >  2:865870/1‑100 (MQ=255)
                                          cgcTACCACGATATTCATTTTGTTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                       >  1:2613958/1‑100 (MQ=255)
                                          cgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                       <  1:2293088/100‑1 (MQ=255)
                                          cgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                       <  2:1308906/100‑1 (MQ=255)
                                          cgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                       <  2:56774/100‑1 (MQ=255)
                                          cgcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCtttt                                                      <  2:2097463/100‑1 (MQ=255)
                                           gttaaaaCGATATTCATTTAGCTGGAAAAGTAAGACGGTGCCATGTTAAAAAAAAAAAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCtttt                                                      <  2:1298042/94‑1 (MQ=255)
                                            cTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTa                                                     <  2:1872866/100‑1 (MQ=255)
                                              aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGc                                                   >  1:1961422/1‑100 (MQ=255)
                                              aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGc                                                   <  2:2010277/100‑1 (MQ=255)
                                               ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCt                                                  >  1:1519162/1‑100 (MQ=255)
                                               ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCt                                                  <  2:207862/100‑1 (MQ=255)
                                               ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCt                                                  >  2:2321210/1‑100 (MQ=255)
                                               ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCt                                                  >  2:2935993/1‑100 (MQ=255)
                                                 aCGATATTCATTTTGCTGGAAAAGTCAGACGGTGCAATGCTGAGCTTCTTCCAAAAAAGTCAGATAGTTCGTTCCCCATCCAACCTCTTCttttcg                                                    >  2:567789/1‑94 (MQ=255)
                                                 aCGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcg                                                >  2:373808/1‑100 (MQ=255)
                                                  cGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCGACCTCTTCTTTTAGCTcgc                                               <  1:561110/100‑1 (MQ=255)
                                                  cGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcgc                                               >  1:164901/1‑100 (MQ=255)
                                                    atatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCgg                                             <  1:1170085/100‑1 (MQ=255)
                                                     tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                            >  2:1213258/1‑100 (MQ=255)
                                                     tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                            <  2:1748162/100‑1 (MQ=255)
                                                     tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                            >  1:2983541/1‑100 (MQ=255)
                                                     tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                            <  2:1520030/100‑1 (MQ=255)
                                                      atTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                           <  2:790993/100‑1 (MQ=255)
                                                       ttCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTa                                          >  2:1012784/1‑100 (MQ=255)
                                                        tCATTTTGCGGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAg                                         <  1:1234207/100‑1 (MQ=255)
                                                         cATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGc                                        <  2:843820/100‑1 (MQ=255)
                                                          aTTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCg                                       >  1:837356/1‑100 (MQ=255)
                                                            tgtGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGc                                     >  1:2064457/3‑100 (MQ=255)
                                                              tGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCtt                                   >  1:2919989/1‑100 (MQ=255)
                                                               gCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTc                                  <  1:2219048/100‑1 (MQ=255)
                                                                ccggAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCg                                 <  1:2572355/98‑1 (MQ=255)
                                                                cTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCg                                 >  2:879158/1‑100 (MQ=255)
                                                                cTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCg                                 <  1:2518430/100‑1 (MQ=255)
                                                                 tGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGt                                >  1:1466084/1‑100 (MQ=255)
                                                                 tGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGt                                <  1:627659/100‑1 (MQ=255)
                                                                    aaaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCGCTTCTTTTAGCTCGCGGTTAGCgtctacgaagc                             <  2:2001999/100‑11 (MQ=255)
                                                                    aaaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGTCtacgaagcc                            >  2:652791/1‑92 (MQ=255)
                                                                     aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGAc                            <  1:2180197/100‑1 (MQ=255)
                                                                     aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGAc                            <  2:1073938/100‑1 (MQ=255)
                                                                     aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGAc                            >  1:3013580/1‑100 (MQ=255)
                                                                       aGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGc                          >  1:2616140/1‑100 (MQ=255)
                                                                        gTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCt                         >  1:84130/1‑100 (MQ=255)
                                                                          aaGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCttt                       >  2:1721847/1‑100 (MQ=255)
                                                                           aGACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTc                      <  1:1137915/100‑1 (MQ=255)
                                                                            gACGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCa                     <  1:2689963/100‑1 (MQ=255)
                                                                              cGGTGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCAcc                   >  1:2203931/1‑100 (MQ=255)
                                                                                 tGCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCgg                >  1:351306/1‑100 (MQ=255)
                                                                                  gCCATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGa               >  1:2439751/1‑100 (MQ=255)
                                                                                   ccATGCTGAGCTCCTTCAAAAAAAGTCAGATCGTTCGCTCCACATCCAACCACTACTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTCTCAACCGGa               >  2:830524/1‑99 (MQ=255)
                                                                                    cATGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATc             <  1:181131/100‑1 (MQ=255)
                                                                                    cATGCTGAGCTTCTTCAAAAAGAGTCAGATCGTTCGCTCCAAATCCAACCTCTGCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATc             <  2:556237/100‑1 (MQ=255)
                                                                                     aTGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCa            <  2:1504620/100‑1 (MQ=255)
                                                                                     aTGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCa            >  1:153163/1‑100 (MQ=255)
                                                                                     aTGCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCa            >  2:1093976/1‑100 (MQ=255)
                                                                                       gCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAt          >  1:3019734/1‑100 (MQ=255)
                                                                                       gCTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAt          >  2:2278276/1‑100 (MQ=255)
                                                                                        cTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAtt         <  1:35457/100‑1 (MQ=255)
                                                                                        cTGAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAtt         <  2:3019693/100‑1 (MQ=255)
                                                                                          gAGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTaa       <  2:2559822/100‑1 (MQ=255)
                                                                                           aGCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAAt      <  2:178352/100‑1 (MQ=255)
                                                                                            gCTTCTTCAAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATc     <  2:2528269/100‑1 (MQ=255)
                                                                                                tcttcaAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt  <  1:205045/100‑1 (MQ=255)
                                                                                                      |                                                                                                 
CTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTT  >  NC_000913/3525721‑3525914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: