Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9580AT23.7% 221.0 / 76.0 114intergenic (+11/+14)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (38/49);  new base T (8/19);  total (46/68)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 123 total aligned reads displayed.

CGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTT  >  NC_000913/1674859‑1675057
                                                                                                   |                                                                                                   
cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                     <  2:2755186/100‑1 (MQ=255)
 gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                    >  1:2156197/1‑100 (MQ=255)
 gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                    >  2:272900/1‑100 (MQ=255)
 gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGACGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                     >  2:420850/1‑99 (MQ=255)
  ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCaga                                                                                                   >  2:2268810/1‑100 (MQ=255)
      cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGcc                                                                                               <  1:343466/100‑1 (MQ=255)
       aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCg                                                                                              <  1:686397/100‑1 (MQ=255)
         ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                            <  2:2218158/100‑1 (MQ=255)
         ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                            <  1:287680/100‑1 (MQ=255)
         ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                            <  1:2252548/100‑1 (MQ=255)
         ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTc                                                                                            <  1:2033413/100‑1 (MQ=255)
          tattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCa                                                                                           <  1:310245/100‑1 (MQ=255)
           attGTCAAAATTGCGCTTGAACAAGCAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                          <  1:2856058/100‑2 (MQ=255)
           attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                          <  2:1917916/100‑2 (MQ=255)
           attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                          <  2:2366155/100‑2 (MQ=255)
           attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                          <  1:447805/100‑2 (MQ=255)
           attGTAAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                          <  2:1437815/100‑2 (MQ=255)
            ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                         <  1:1801529/100‑3 (MQ=255)
            ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                         <  1:2434246/100‑3 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                        >  1:890796/1‑100 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                        <  1:804449/100‑1 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                        <  1:2080734/100‑1 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                        <  1:2310536/100‑1 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                        >  1:1643693/1‑100 (MQ=255)
                  aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactctttat                                                                                   <  2:215101/100‑9 (MQ=38)
                  aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACa                                                                                   <  2:1460045/100‑1 (MQ=255)
                  aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACa                                                                                   >  2:1539029/1‑100 (MQ=255)
                   aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                                  <  1:2788162/100‑1 (MQ=255)
                    aTTGCGCTTAAACAAACAGCGGGATGTCAAAGCATCGCCGTTCTGTTAAGCAATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCactctttattg                                                                                 <  1:1374934/100‑11 (MQ=38)
                     ttGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                                <  2:2612896/100‑1 (MQ=255)
                        cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                             >  1:1388215/1‑100 (MQ=255)
                          cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                           <  2:2939534/100‑1 (MQ=255)
                          cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                           <  2:343145/100‑1 (MQ=255)
                          cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                           >  2:704236/1‑100 (MQ=255)
                            tGAACACACAGCGGGAGGTTAATGTATTGTTGTTCTGTTAAGCGATCACAATAAAGAGTGTCTGCCTCAGTATAGGCCGTCAGGGTTACagag                                                                                <  2:2549521/93‑1 (MQ=255)
                            ngAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAg                                                                         >  1:706665/2‑100 (MQ=255)
                             gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAgg                                                                        >  2:1000600/1‑100 (MQ=255)
                             gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAgg                                                                        <  2:1420010/100‑1 (MQ=255)
                             gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAg                                                                         >  2:2933877/1‑99 (MQ=255)
                               ncaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTACGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGATACAGAGCTTTCAg                                                                         >  2:1810035/2‑97 (MQ=255)
                                 agacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtag                                                                    >  1:2065171/3‑98 (MQ=255)
                                  aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                   >  2:1259362/1‑100 (MQ=255)
                                    caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAt                                                                 >  2:2198937/1‑100 (MQ=255)
                                       cGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCa                                                              <  2:2421860/100‑1 (MQ=255)
                                       cGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCa                                                              <  2:199505/100‑1 (MQ=255)
                                         ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                            <  1:354419/100‑1 (MQ=255)
                                           aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                          <  1:1880877/100‑1 (MQ=255)
                                           aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                          <  1:1881068/100‑1 (MQ=255)
                                           aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                          <  1:2923165/100‑1 (MQ=255)
                                           aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                          <  2:826569/100‑1 (MQ=255)
                                             gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgn                                                        <  2:1293707/100‑2 (MQ=255)
                                              tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                       <  2:1752303/100‑1 (MQ=255)
                                               cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                      >  2:1657026/1‑100 (MQ=255)
                                                aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt                                                     <  1:1607011/100‑1 (MQ=255)
                                                aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt                                                     >  2:1217444/1‑100 (MQ=255)
                                                   gCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                                  <  1:229104/100‑1 (MQ=255)
                                                          cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAcc                                           <  1:1551767/100‑1 (MQ=255)
                                                          cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAcc                                           <  2:2772912/100‑1 (MQ=255)
                                                            ttCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaa                                         <  2:1028958/100‑1 (MQ=255)
                                                             tCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaaa                                        <  1:1539047/100‑1 (MQ=255)
                                                              cTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAAc                                       <  1:2326922/100‑1 (MQ=255)
                                                               tGTTAAGCGATCTCAATAAAAAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                      >  2:1867775/1‑100 (MQ=255)
                                                                gTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGACAGGGATACAAAGCGTTCAGGATTGCATCCACGCTGGCTTTGGCGTCCCCAAACa                                      >  2:1030120/1‑99 (MQ=255)
                                                                   aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAt                                  >  2:2114675/1‑100 (MQ=255)
                                                                    aGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtg                                 <  2:1188644/100‑1 (MQ=255)
                                                                    aGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGACTTTGGCGTCACCAAACAGCAt                                  >  2:1368553/1‑99 (MQ=255)
                                                                      cGATCACAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgtg                               <  1:2904427/100‑1 (MQ=255)
                                                                       gATCTCAATACAGGGTGACGGCCTCAGCAGAGGACGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                              <  2:137196/100‑1 (MQ=255)
                                                                       gATCTCAATAAAGAGTGCCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGGTTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                              >  2:1456053/1‑100 (MQ=255)
                                                                        aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                             <  2:1388202/100‑1 (MQ=255)
                                                                          ctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg                           >  2:56837/1‑100 (MQ=255)
                                                                          ctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg                           >  1:494577/1‑100 (MQ=255)
                                                                            cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                         >  2:1399438/1‑100 (MQ=255)
                                                                            cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                         >  2:2642711/1‑100 (MQ=255)
                                                                               tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc                      >  1:136940/1‑100 (MQ=255)
                                                                               tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc                      >  1:1024639/1‑100 (MQ=255)
                                                                                aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                     <  1:3021456/100‑1 (MQ=255)
                                                                                  accctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                   >  1:797080/5‑100 (MQ=39)
                                                                                   gagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                  <  1:2273867/100‑1 (MQ=255)
                                                                                   gagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                  >  1:1064623/1‑100 (MQ=255)
                                                                                   ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                  >  1:986777/4‑100 (MQ=255)
                                                                                   ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                  >  1:2326527/4‑100 (MQ=39)
                                                                                   ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAAGTGGGTGTTTTCCTTg                  >  2:1791534/4‑100 (MQ=38)
                                                                                   ccctGACGGCCTCGGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                  >  2:2095402/4‑100 (MQ=255)
                                                                                    cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga                 >  1:1082775/3‑100 (MQ=255)
                                                                                    agTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga                 <  2:800082/100‑1 (MQ=255)
                                                                                     gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCGTCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa                <  2:2939957/100‑1 (MQ=255)
                                                                                     gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa                <  1:1031844/100‑1 (MQ=255)
                                                                                     gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa                <  1:1355727/100‑1 (MQ=255)
                                                                                     gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTAAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga                 <  2:1332349/99‑1 (MQ=255)
                                                                                     ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa                >  1:202671/2‑100 (MQ=255)
                                                                                       gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga              <  2:453327/100‑1 (MQ=255)
                                                                                       gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga              <  2:494570/100‑1 (MQ=255)
                                                                                       gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga              >  1:1034019/1‑100 (MQ=255)
                                                                                          ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa           <  2:2813763/100‑1 (MQ=255)
                                                                                          ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa           <  1:3043244/100‑1 (MQ=255)
                                                                                           gCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAg          >  1:432380/1‑100 (MQ=255)
                                                                                            ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc         >  2:1908983/1‑100 (MQ=255)
                                                                                               cagcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg       <  1:1867797/99‑1 (MQ=255)
                                                                                                agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGt     <  1:1681199/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTT  >  NC_000913/1674859‑1675057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑