Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,246,073	C→T	G435D (GGC→GAC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,073	0	C	T	98.9%	307.4 / ‑5.4	93	G435D (GGC→GAC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (49/43);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245979‑1246168                                                                                               |                                                                                                gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            >  1:2871165/1‑100 (MQ=255) gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            >  2:2160562/1‑100 (MQ=255) gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            >  2:1174390/1‑100 (MQ=255)  ttttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCgg                                                                                           <  1:297892/100‑1 (MQ=255)    ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         >  2:211373/1‑100 (MQ=255)    ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         <  1:197916/100‑1 (MQ=255)     tGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGtt                                                                                        <  1:2160591/100‑1 (MQ=255)     tGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGtt                                                                                        <  2:2587086/100‑1 (MQ=255)      gTAATCCTTCTGTCGCGACCGACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTg                                                                                       >  2:1866837/1‑100 (MQ=255)      gTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTg                                                                                       >  1:2400744/1‑100 (MQ=255)       tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                      >  1:397727/1‑100 (MQ=255)       tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                      >  2:1405689/1‑100 (MQ=255)       tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                      <  1:1264690/100‑1 (MQ=255)        aaTCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgca                                                                                     <  2:1461174/100‑1 (MQ=255)         aTCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcag                                                                                    >  2:2321587/1‑100 (MQ=255)         aTCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcag                                                                                    <  1:2251062/100‑1 (MQ=255)          tCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagc                                                                                   >  2:1224022/1‑100 (MQ=255)           ccTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagca                                                                                  <  2:640211/100‑1 (MQ=255)             ttCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGa                                                                                <  2:972537/100‑1 (MQ=255)              tCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAt                                                                               <  1:1347992/100‑1 (MQ=255)                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                            <  1:61992/100‑1 (MQ=255)                   cgcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATgtgt                                                                           >  1:1859039/1‑99 (MQ=255)                    gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGttt                                                                         >  2:1140867/1‑100 (MQ=255)                       aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                      <  1:1174402/100‑1 (MQ=255)                        cccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgt                                                                     >  2:570529/1‑100 (MQ=255)                        cccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgt                                                                     >  2:3032416/1‑100 (MQ=255)                        cccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCAGTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATgtgt                                                                           <  2:3021452/94‑1 (MQ=255)                         ccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                                                    <  2:9251/100‑1 (MQ=255)                          cACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                                   >  1:1017364/1‑100 (MQ=255)                          cACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                                   >  1:2525716/1‑100 (MQ=255)                             tGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                                >  2:2959241/1‑100 (MQ=255)                             tGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                                <  1:1685706/100‑1 (MQ=255)                               tAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtc                                                              <  2:1546712/100‑1 (MQ=255)                               tAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtc                                                              <  1:1950771/100‑1 (MQ=255)                                aaCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcg                                                             >  2:1979603/1‑100 (MQ=255)                                aaCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTCcgtagccgtcg                                                             <  2:1609817/100‑1 (MQ=255)                                 aCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGCGTTTTcgtcgccgtcgc                                                            >  1:2339225/1‑100 (MQ=255)                                  cGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTAGCCGGGTTGCAGCAGATGTGTTTTCgtcgccgtcgcc                                                           >  1:1784436/1‑100 (MQ=255)                                     tGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtt                                                        >  2:2579466/1‑100 (MQ=255)                                      gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                       >  1:2797414/1‑100 (MQ=255)                                      gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTCGCAGCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                       <  1:2746343/100‑1 (MQ=255)                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCCGGTCGCCGGGTTTCACCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                       >  2:2880818/1‑98 (MQ=255)                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     >  2:461642/1‑100 (MQ=255)                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     <  1:2208233/100‑1 (MQ=255)                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     >  2:172388/1‑100 (MQ=255)                                         cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCCGGTCGCCGGGTTGCAGAAGATGTGTTTTCGTGGCCGTCGCCATTttg                                                     >  2:2799143/1‑99 (MQ=255)                                         cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    >  2:169966/1‑100 (MQ=255)                                         cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    <  2:2244017/100‑1 (MQ=255)                                          aGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgtt                                                   <  1:846110/100‑1 (MQ=255)                                          aGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgtt                                                   <  2:2855756/100‑1 (MQ=255)                                           gCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgttg                                                  >  1:1105975/1‑100 (MQ=255)                                              aTTTGGCGCATCACATTGTTGCTCACGTGTCACCTCCGTGGTGTTCATGTCGCCGTGTTGCAGCTGATGTGTTTTCGTCGCCGTCGCTATGttgttg                                                  <  2:1667440/97‑1 (MQ=255)                                                 tGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGa                                            >  1:1103835/1‑100 (MQ=255)                                                  ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                           <  2:397720/100‑1 (MQ=255)                                                    cgcATCCCATTGTTGCCCACTTTTCACCGGCGTTGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATc                                          <  2:593338/99‑1 (MQ=255)                                                     gcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCtt                                        >  1:1378047/1‑100 (MQ=255)                                                        tCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                                     <  1:2726904/100‑1 (MQ=255)                                                        tCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                                     <  2:2400713/100‑1 (MQ=255)                                                           cATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                                  >  2:983377/1‑100 (MQ=255)                                                           cATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                                  >  2:243542/1‑100 (MQ=255)                                                           cATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                                  >  2:1906085/1‑100 (MQ=255)                                                             ttgttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGcc                                <  1:1552319/100‑1 (MQ=255)                                                               gttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGc                              >  1:213182/1‑100 (MQ=255)                                                                ttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                             <  2:2176268/100‑1 (MQ=255)                                                                ttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                             <  1:181970/100‑1 (MQ=255)                                                                 tgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                            <  1:1094900/100‑1 (MQ=255)                                                                 tgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                            >  2:478461/1‑100 (MQ=255)                                                                 tgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                            >  1:2916268/1‑100 (MQ=255)                                                                 tgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                            >  2:2679861/1‑100 (MQ=255)                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                           >  1:533179/1‑100 (MQ=255)                                                                   cccACTTTTCACCGACGTTGTGTTCTGGTCGCCGTGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg                          <  2:1000324/100‑1 (MQ=255)                                                                   cccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg                          <  2:198723/100‑1 (MQ=255)                                                                    ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         >  2:322750/1‑100 (MQ=255)                                                                    ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         >  2:2465424/1‑100 (MQ=255)                                                                    ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         <  2:2663993/100‑1 (MQ=255)                                                                     cACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGAc                        <  1:386320/100‑1 (MQ=255)                                                                      aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg                          <  2:887075/97‑1 (MQ=255)                                                                       ctgcgcACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       <  2:19597/94‑1 (MQ=255)                                                                       cTTTTCAACGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGt                      >  1:1612645/1‑100 (MQ=255)                                                                          ttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa                   <  1:1224034/100‑1 (MQ=255)                                                                          ttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa                   >  2:1582699/1‑100 (MQ=255)                                                                          tgcACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTAc                    <  2:129454/97‑1 (MQ=255)                                                                            cACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGc                 <  2:2836171/100‑1 (MQ=255)                                                                            cACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGc                 >  2:700853/1‑100 (MQ=255)                                                                              ccGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCgg               >  1:184153/1‑100 (MQ=255)                                                                               cGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCggg              <  1:1140879/100‑1 (MQ=255)                                                                                  cGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTACCGCATTGACGTACAGCGGGAAc           <  1:436683/100‑1 (MQ=255)                                                                                    tggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg         <  2:2792078/100‑1 (MQ=255)                                                                                    tggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg         >  2:1191528/1‑100 (MQ=255)                                                                                     ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg        >  2:1602968/1‑100 (MQ=255)                                                                                     ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg        >  2:806162/1‑100 (MQ=255)                                                                                       tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc      >  2:2950796/1‑100 (MQ=255)                                                                                           cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  2:148990/1‑100 (MQ=255)                                                                                           cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  2:1334912/1‑100 (MQ=255)                                                                                           cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  2:1261930/1‑100 (MQ=255)                                                                                           cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  1:702376/1‑100 (MQ=255)                                                                                               |                                                                                                GTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245979‑1246168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑