Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,868,250 A→G Y448C (TAC→TGC)  yeaG → protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,2500AG99.1% 372.4 / ‑4.8 105Y448C (TAC→TGC) yeaGprotein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (45/59);  total (45/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 105 total aligned reads displayed.

ATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGC  >  NC_000913/1868153‑1868346
                                                                                                 |                                                                                                
aTGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCg                                                                                                <  1:2781200/100‑1 (MQ=255)
aTGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCg                                                                                                <  2:1329492/100‑1 (MQ=255)
 tGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGt                                                                                               >  1:481861/1‑100 (MQ=255)
  gAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTc                                                                                              <  2:2810456/100‑1 (MQ=255)
   aaCGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTcc                                                                                             <  1:975900/100‑1 (MQ=255)
     cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                           <  2:2661495/100‑1 (MQ=255)
     cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                           <  1:177634/100‑1 (MQ=255)
      ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                          >  1:807609/1‑100 (MQ=255)
      ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTgg                                                                                          <  2:2621826/100‑1 (MQ=255)
       gTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTTGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGa                                                                                         <  1:2285285/100‑1 (MQ=255)
        tCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGaa                                                                                        <  2:1749626/100‑1 (MQ=255)
         cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                       >  2:1362867/1‑100 (MQ=255)
         cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                       <  1:1520446/100‑1 (MQ=255)
          tGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAca                                                                                      <  2:1169760/100‑1 (MQ=255)
           gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcag                                                                                     >  2:183421/1‑100 (MQ=255)
           gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcag                                                                                     >  1:1694231/1‑100 (MQ=255)
           gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcag                                                                                     >  1:2693121/1‑100 (MQ=255)
           gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcaa                                                                                     >  1:1169772/1‑99 (MQ=255)
            tCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagc                                                                                    <  1:2692049/100‑1 (MQ=255)
            tCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagc                                                                                    <  2:1050222/100‑1 (MQ=255)
              gACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagcag                                                                                  <  1:10931/100‑1 (MQ=255)
               aCGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGa                                                                                 >  2:191926/1‑100 (MQ=255)
               aCGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGa                                                                                 >  1:2282605/1‑100 (MQ=255)
                 gcgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                               >  2:579119/1‑100 (MQ=255)
                  cgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGAGCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAttn                                                                              <  2:332814/100‑2 (MQ=255)
                   gTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGa                                                                             >  1:60871/1‑100 (MQ=255)
                    ttttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAg                                                                            >  2:2673952/1‑100 (MQ=255)
                       tGCGTTTGAGAGCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgcgc                                                                         >  2:1603405/1‑100 (MQ=255)
                        gCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg                                                                        >  1:2171074/1‑100 (MQ=255)
                        gCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg                                                                        <  1:840175/100‑1 (MQ=255)
                        gCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg                                                                        >  2:671341/1‑100 (MQ=255)
                        gCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg                                                                        <  2:412239/100‑1 (MQ=255)
                        gCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg                                                                        >  1:152919/1‑100 (MQ=255)
                        gCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg                                                                        >  1:1980013/1‑100 (MQ=255)
                         cGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGa                                                                       >  1:1751068/1‑100 (MQ=255)
                         cGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGa                                                                       >  1:1052407/1‑100 (MQ=255)
                          gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                      <  2:89859/100‑1 (MQ=255)
                             tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                   >  2:357901/1‑100 (MQ=255)
                             tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                   <  1:1717467/100‑1 (MQ=255)
                             tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                   <  1:1378713/100‑1 (MQ=255)
                             tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                   <  2:2424439/100‑1 (MQ=255)
                             tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                   <  1:600096/100‑1 (MQ=255)
                             tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                   <  1:183428/100‑1 (MQ=255)
                               aGATCCTCTCCCGCGTGTTAAACTTAGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGtt                                                                 >  2:1778627/1‑100 (MQ=255)
                                gATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTc                                                                >  2:2272532/1‑100 (MQ=255)
                                gATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTc                                                                <  2:536531/100‑1 (MQ=255)
                                gATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTc                                                                <  1:2075995/100‑1 (MQ=255)
                                  tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc                                                              <  1:1318483/100‑1 (MQ=255)
                                   ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc                                                              <  2:398179/99‑1 (MQ=255)
                                    ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcac                                                            <  2:936293/100‑1 (MQ=255)
                                     tctcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcaca                                                           <  1:2272561/100‑1 (MQ=255)
                                      ctcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACaa                                                          >  2:2987283/1‑100 (MQ=255)
                                       tcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCTGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag                                                         <  2:1946904/100‑1 (MQ=255)
                                       tcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag                                                         <  2:1994695/100‑1 (MQ=255)
                                         ccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                       <  1:1034037/100‑1 (MQ=255)
                                           gcgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgca                                                     <  2:927629/100‑1 (MQ=255)
                                             gtgtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcagg                                                   <  2:2524751/100‑1 (MQ=255)
                                              tgtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggc                                                  <  1:1284061/100‑1 (MQ=255)
                                               gtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGCACCAGCAGATTGAGCGCGAGCAGTGCCCACAAGAgcaggca                                                 <  2:1878086/100‑1 (MQ=255)
                                                ttCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggcag                                                >  1:1380740/1‑100 (MQ=255)
                                                ttCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggcag                                                <  1:2852694/100‑1 (MQ=255)
                                                    aCTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACg                                            <  2:2523622/100‑1 (MQ=255)
                                                    aCTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACg                                            >  1:333543/1‑100 (MQ=255)
                                                     cTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGc                                           >  1:1969383/1‑100 (MQ=255)
                                                       tCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTa                                         <  1:191933/100‑1 (MQ=255)
                                                        cGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTAt                                        >  1:311917/1‑100 (MQ=255)
                                                        cGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTAt                                        >  1:2545776/1‑100 (MQ=255)
                                                        cGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTAt                                        >  2:2862356/1‑100 (MQ=255)
                                                         gATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATc                                       <  2:1124990/100‑1 (MQ=255)
                                                          aTCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCt                                      >  2:66850/1‑100 (MQ=255)
                                                          aTCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCt                                      <  2:3036884/100‑1 (MQ=255)
                                                          aTCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCt                                      <  2:2282576/100‑1 (MQ=255)
                                                            cATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTgg                                    <  1:2859180/100‑1 (MQ=255)
                                                              tGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                  <  2:1297391/100‑1 (MQ=255)
                                                              tGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                  <  1:1362880/100‑1 (MQ=255)
                                                              tGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                  <  2:583703/100‑1 (MQ=255)
                                                               gTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGt                                 >  2:2810816/1‑100 (MQ=255)
                                                                 ngAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTc                               >  2:2575354/2‑100 (MQ=255)
                                                                  gAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTGcc                              <  2:1100354/100‑1 (MQ=255)
                                                                     gTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGa                           >  1:1142754/1‑100 (MQ=255)
                                                                       ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa                         <  2:1573790/100‑1 (MQ=255)
                                                                        gcagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAg                        >  1:258916/1‑100 (MQ=255)
                                                                        gcagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAg                        <  2:389526/100‑1 (MQ=255)
                                                                         cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                       <  2:2515264/100‑1 (MQ=255)
                                                                         cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                       >  2:2241723/1‑100 (MQ=255)
                                                                              aaCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATc                  <  1:516848/100‑1 (MQ=255)
                                                                               aCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt                 <  1:1659739/100‑1 (MQ=255)
                                                                                cccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTg                >  1:2424189/1‑100 (MQ=255)
                                                                                cccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTg                <  2:1712025/100‑1 (MQ=255)
                                                                                 ccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGa               >  1:1085275/1‑100 (MQ=255)
                                                                                 ccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGa               >  2:1447876/1‑100 (MQ=255)
                                                                                       cATCTGTTCTGCGTCCTGGAACAGCAGATTGAGGGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGa         >  1:2523964/1‑100 (MQ=255)
                                                                                       cATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGa         >  1:1372666/1‑100 (MQ=255)
                                                                                       cATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGa         >  1:2841916/1‑100 (MQ=255)
                                                                                        aTCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaa        >  1:2842464/1‑100 (MQ=255)
                                                                                          cTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAat      <  2:791454/100‑1 (MQ=255)
                                                                                          cTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAat      <  2:887249/100‑1 (MQ=255)
                                                                                           tGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAata     <  2:2672268/100‑1 (MQ=255)
                                                                                           tGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAat      <  2:1052396/99‑1 (MQ=255)
                                                                                              tCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGc  >  1:225338/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGC  >  NC_000913/1868153‑1868346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑