Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,702	T→C	L329P (CTG→CCG)	yeiH →	UPF0324 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,702	0	T	C	100.0%	323.6 / NA	90	L329P (CTG→CCG)	yeiH	UPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/45);  total (45/45)

TGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCT  >  NC_000913/2250609‑2250796                                                                                              |                                                                                               tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGc                                                                                          >  1:631168/1‑100 (MQ=255)  gctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGcc                                                                                         >  1:1251357/1‑100 (MQ=255)    tgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTg                                                                                       <  2:1381690/100‑1 (MQ=255)     ggCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTgg                                                                                      <  2:748881/100‑1 (MQ=255)      gCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGc                                                                                     >  1:934390/1‑100 (MQ=255)       cAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCt                                                                                    <  2:388472/100‑1 (MQ=255)       cAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCt                                                                                    <  2:1229220/100‑1 (MQ=255)       cAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCt                                                                                    >  2:2180722/1‑100 (MQ=255)        aaTGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTg                                                                                   <  1:950972/100‑1 (MQ=255)        aaTGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTg                                                                                   >  2:3046069/1‑100 (MQ=255)          tggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTg                                                                                   >  2:407411/1‑98 (MQ=255)           ggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGAtt                                                                                <  1:1071342/100‑1 (MQ=255)           ggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGAtt                                                                                >  1:2699539/1‑100 (MQ=255)            gcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGAttg                                                                               <  1:1829/100‑1 (MQ=255)            gcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGAttg                                                                               <  2:1034533/100‑1 (MQ=255)             cgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGAttgt                                                                              <  2:3023905/100‑1 (MQ=255)                 ggcggcGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTtggt                                                                          >  2:2650185/1‑100 (MQ=255)                 ggcggcGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTtggt                                                                          >  1:1210660/1‑100 (MQ=255)                    ggcgCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTtggtggt                                                                       >  2:77168/1‑100 (MQ=255)                      cgcTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTtggtggtgg                                                                     <  1:1253268/100‑1 (MQ=255)                      cgcTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCATGGCTGATTGTtggtggtgg                                                                     >  1:518005/1‑100 (MQ=255)                         tGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                                                  <  1:1126432/100‑1 (MQ=255)                          gggtgggACCACGCACGGCAGCGCGCTGAAAAAAGCTGGGGGGAAACCTCTGCTGGTGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                                 <  1:2617935/94‑1 (MQ=255)                          gggTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                                 <  2:577806/100‑1 (MQ=255)                          gggTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                                 >  1:1894978/1‑100 (MQ=255)                            ntCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                                               >  2:2151571/2‑100 (MQ=255)                            gTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                                               <  1:361682/100‑1 (MQ=255)                             tCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAtt                                                              <  2:1719470/100‑1 (MQ=255)                              cTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAtt                                                              <  2:1722345/99‑1 (MQ=255)                              cTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTa                                                             >  1:1900650/1‑100 (MQ=255)                               tGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTaa                                                            >  1:2451467/1‑100 (MQ=255)                                ggccgctggcGTTAGCGTGCTGAAAGAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTAGTGCGATTAAc                                                           <  2:560364/91‑1 (MQ=255)                                gACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTaan                                                           <  1:792522/100‑2 (MQ=255)                                  ccACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTa                                                         >  1:1312145/1‑100 (MQ=255)                                    tcTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtg                                                       <  2:911870/99‑1 (MQ=255)                                     cTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGCTGGCACTGGTACCATTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtgt                                                      >  2:1419626/1‑100 (MQ=255)                                     cTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtga                                                      >  1:911880/1‑99 (MQ=255)                                     cTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtg                                                       <  2:1806379/99‑1 (MQ=255)                                        aCGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAt                                                   <  1:1859424/100‑1 (MQ=255)                                          gTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGTCTGACTGTTGGGGGGGGTGCGAGTGACTATGTGATTc                                                 >  2:1014200/1‑100 (MQ=255)                                          gTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTc                                                 <  2:1442105/100‑1 (MQ=255)                                            cAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCaa                                               >  1:1173573/1‑100 (MQ=255)                                             aGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCaaa                                              <  2:1821219/100‑1 (MQ=255)                                             aGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCaaa                                              >  1:1341858/1‑100 (MQ=255)                                             aGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCaaa                                              >  1:1341792/1‑100 (MQ=255)                                               cgcgcTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAg                                             <  2:2433948/99‑1 (MQ=255)                                               cgcgcTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGc                                            >  1:1253477/1‑100 (MQ=255)                                                gcgcTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCg                                           <  1:2861479/100‑1 (MQ=255)                                                 cgcTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGt                                          >  1:1520555/1‑100 (MQ=255)                                                    tGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCAt                                       >  1:2837747/1‑100 (MQ=255)                                                     gAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATc                                      >  1:306585/1‑100 (MQ=255)                                                     gAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATc                                      >  2:1549885/1‑100 (MQ=255)                                                     gAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATc                                      >  2:1357386/1‑100 (MQ=255)                                                     gAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATc                                      <  2:643191/100‑1 (MQ=255)                                                      aaaaaaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCg                                     <  2:1004397/100‑1 (MQ=255)                                                      aaaaaaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCg                                     <  2:875136/100‑1 (MQ=255)                                                      aaaaaaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCg                                     >  2:1925375/1‑100 (MQ=255)                                                       aaaaaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGc                                    >  1:1226404/1‑100 (MQ=255)                                                         aaaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCAt                                  <  1:2180751/100‑1 (MQ=255)                                                          aaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATa                                 <  2:1396520/100‑1 (MQ=255)                                                            gCTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATaaa                               <  1:2581400/100‑1 (MQ=255)                                                             cTGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAc                              >  2:1624710/1‑100 (MQ=255)                                                              tGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAcc                             <  2:2695966/100‑1 (MQ=255)                                                              tGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAcc                             <  1:2774104/100‑1 (MQ=255)                                                              tGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAcc                             >  1:2585907/1‑100 (MQ=255)                                                              tGGGGCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAcc                             <  2:120089/100‑1 (MQ=255)                                                               gggtCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCa                            >  2:901483/1‑100 (MQ=255)                                                                gggCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCAc                           <  2:2699502/100‑1 (MQ=255)                                                                 ggCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACt                          <  2:1251345/100‑1 (MQ=255)                                                                 ggCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACt                          >  1:1641931/1‑100 (MQ=255)                                                                 ggCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGATGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACt                          >  2:1953089/1‑100 (MQ=255)                                                                  gCGAAACCTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTa                         <  2:328105/100‑1 (MQ=255)                                                                        ccTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCtt                   <  1:325954/100‑1 (MQ=255)                                                                        ccTCTGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCtt                   <  1:739328/100‑1 (MQ=255)                                                                          tctGCTGATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc                 >  2:2803484/1‑100 (MQ=255)                                                                             gctgATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTcc              >  1:549748/1‑100 (MQ=255)                                                                              ctgATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCt             >  1:2726269/1‑100 (MQ=255)                                                                              ctgATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCt             <  1:1947741/100‑1 (MQ=255)                                                                                gATGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGt           <  1:3046112/100‑1 (MQ=255)                                                                                 aTGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGtt          <  2:2726232/100‑1 (MQ=255)                                                                                 aTGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGtt          <  2:2228720/100‑1 (MQ=255)                                                                                  tGGCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTa         >  1:2325806/1‑100 (MQ=255)                                                                                   ggCACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTaa        >  2:833636/1‑100 (MQ=255)                                                                                     cACTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAAcc      >  1:797041/1‑100 (MQ=255)                                                                                      aCTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAAccc     <  1:2502745/100‑1 (MQ=255)                                                                                      aCTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAAccc     >  1:160762/1‑100 (MQ=255)                                                                                       cTGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCg    >  1:15679/1‑100 (MQ=255)                                                                                        tGGTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTACTGTTAAccc     >  1:202748/1‑98 (MQ=255)                                                                                        tGGTACCGTTTGCCTGGCTGAATGTTGGTGGTCGTGCTATTAACTATGTGATTAAAAGAGTCATCGCATAAACCACTACATCTTACTCCTGTTAAccc     >  2:2570570/1‑98 (MQ=255)                                                                                          gTACCGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCt  <  1:3040552/99‑1 (MQ=255)                                                                                              |                                                                                               TGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCT  >  NC_000913/2250609‑2250796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑