Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,312	G→A	intergenic (‑155/+288)	rrsG ← / ← clpB	16S ribosomal RNA of rrnG operon/protein disaggregation chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,312	0	G	A	100.0%	176.2 / NA	56	intergenic (‑155/+288)	rrsG/clpB	16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/32);  total (24/32)

CATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCC  >  NC_000913/2731225‑2731408                                                                                        |                                                                                                 cATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGATTGCCAGCACCGATTTTCTGACTGATGGATACAGAGCAGTCAcgcctcgattatggagc                                                                                        >  2:2741224/1‑91 (MQ=0)                                                  aGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTc                                     <  1:1710652/100‑1 (MQ=11)                                                  aGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCATTCCCGCTACAGAGTCAAGCATTTc                                     <  2:405586/100‑1 (MQ=2)                                                   gATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCt                                    <  2:2570763/100‑1 (MQ=14)                                                     ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt                                  <  1:1379291/100‑1 (MQ=17)                                                     ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt                                  <  1:628053/100‑1 (MQ=17)                                                      tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                 <  1:1389393/100‑1 (MQ=18)                                                      tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                 <  1:164617/100‑1 (MQ=18)                                                       gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                                <  1:1769249/100‑1 (MQ=25)                                                        tCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCt                               <  1:1995401/100‑1 (MQ=255)                                                          tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACATAGTCAAGCATTTCTTTGCttt                             <  2:743622/100‑1 (MQ=255)                                                          tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                             >  1:1125050/1‑100 (MQ=255)                                                          tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                             <  1:2047572/100‑1 (MQ=255)                                                          tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                             >  1:1283432/1‑100 (MQ=255)                                                          tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTAAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                             >  1:678260/1‑100 (MQ=255)                                                           gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                            >  2:1768971/1‑100 (MQ=255)                                                            atataTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                           <  1:1568194/96‑1 (MQ=255)                                                            aTAAATTGTTAAAGAGTAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                           <  1:2583796/100‑1 (MQ=255)                                                            aTAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                           <  1:1164152/100‑1 (MQ=255)                                                            aTAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGGCAAGCATTTCTTTGCTTTtc                           >  1:885094/1‑100 (MQ=255)                                                             tAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                          <  2:766139/100‑1 (MQ=255)                                                             tAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                          <  2:1007315/100‑1 (MQ=255)                                                              aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGTGGCGCGGGGTGTGCATAATTCGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                         <  2:1667010/100‑1 (MQ=255)                                                              aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                         >  2:903027/1‑100 (MQ=255)                                                              aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                         <  1:1373643/100‑1 (MQ=255)                                                              aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                         <  1:712063/100‑1 (MQ=255)                                                              aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGCGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                         <  2:2657454/100‑1 (MQ=255)                                                               aaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                        <  1:1745762/100‑1 (MQ=255)                                                                 ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt                      >  1:1180363/1‑100 (MQ=255)                                                                  tGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                     <  2:97315/100‑1 (MQ=255)                                                                  tGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                     >  1:75837/1‑100 (MQ=255)                                                                   gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                    >  2:1381568/1‑100 (MQ=255)                                                                   gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                    <  2:2534831/100‑1 (MQ=255)                                                                   gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                    >  1:1493593/1‑100 (MQ=255)                                                                    ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                   <  2:537964/100‑1 (MQ=255)                                                                    ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                   >  1:431006/1‑100 (MQ=255)                                                                    ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                   <  2:2781931/100‑1 (MQ=255)                                                                     tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCCAGCATTTCTTTGCTTTTCTCTGTTgag                  >  1:891618/1‑100 (MQ=255)                                                                     tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCACGCATTTCTTTGCTTTTCTCTGTTgag                  <  1:1806510/100‑1 (MQ=255)                                                                     tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag                  >  1:1341235/1‑100 (MQ=255)                                                                      aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                 >  2:2488668/1‑100 (MQ=255)                                                                       aaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACCGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt                >  1:613898/1‑100 (MQ=255)                                                                          agCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct             >  1:2271331/1‑100 (MQ=255)                                                                           gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc            >  1:1893924/1‑100 (MQ=255)                                                                            cAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa           <  2:2851079/100‑1 (MQ=255)                                                                             aGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg          <  1:375470/100‑1 (MQ=255)                                                                              gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTTCATAATACGCCTTCCCGCTATAGAGTCAAGAATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg         <  2:1326145/100‑1 (MQ=18)                                                                              gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg         >  1:1415733/1‑100 (MQ=255)                                                                              gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg         >  1:2211263/1‑100 (MQ=255)                                                                              gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg         >  2:2320464/1‑100 (MQ=255)                                                                               tgtcgcttcacttTTTCTCAGCGGCGCGGGGTGTGCATAATACTCCTTACCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga        <  1:2108446/97‑1 (MQ=18)                                                                               tGCCGCTTCACTTTTTCTCAGCGGCGAGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga        >  1:1859226/1‑100 (MQ=255)                                                                               tGCCGCTTCACTTTTTCTCAGCGGCGAGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga        >  1:1859233/1‑100 (MQ=255)                                                                                gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag       <  2:1791507/100‑1 (MQ=255)                                                                                gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag       <  2:443649/100‑1 (MQ=255)                                                                                gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag       <  2:51306/100‑1 (MQ=255)                                                                                gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag       >  2:1186511/1‑100 (MQ=255)                                                                                 ccGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga      >  1:303592/1‑100 (MQ=255)                                                                                  cgcttcacttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa     >  2:1379906/1‑100 (MQ=255)                                                                                   gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc    <  2:1596811/100‑1 (MQ=255)                                                                                    cttcacttTTTCTCACCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc   <  1:3011663/100‑1 (MQ=255)                                                                                     ttcacttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcca  >  2:461910/1‑99 (MQ=255)                                                                                        |                                                                                                 CATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCC  >  NC_000913/2731225‑2731408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑