Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,008	G→A	T110I (ACT→ATT)	yhfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,008	0	G	A	100.0%	323.9 / NA	94	T110I (ACT→ATT)	yhfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/45);  total (49/45)

TGCAAAAGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACC  >  NC_000913/3506911‑3507104                                                                                                  |                                                                                                 tGCAAAAGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATa                                                                                                >  2:2901764/1‑100 (MQ=255)  gCAAAAGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATaa                                                                                               >  2:108843/1‑100 (MQ=255)   cAAAAGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAg                                                                                              >  2:3055925/1‑100 (MQ=255)   cAAAAGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAg                                                                                              >  2:1385747/1‑100 (MQ=255)      aaGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGAt                                                                                           >  1:770813/1‑100 (MQ=255)       aGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATc                                                                                          <  2:1251716/100‑1 (MQ=255)       aGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACAGCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATc                                                                                          <  2:287514/100‑1 (MQ=255)          tGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAac                                                                                       >  2:2447692/1‑100 (MQ=255)          tGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCCCGTTTACTGACAACAGGCAAATAAGGATCAac                                                                                       >  2:2059964/1‑100 (MQ=255)           gAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAaca                                                                                      <  1:429466/100‑1 (MQ=255)            aaaCCACCGGCGAGCTTAATTCCCCCAGGCTGCCTAACACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAacac                                                                                     <  2:2051914/100‑1 (MQ=255)               ccaccGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGcc                                                                                  <  1:2393072/100‑1 (MQ=255)               ccaccGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGcc                                                                                  >  2:601001/1‑100 (MQ=255)                   cggctggCTGAATTCCCCCGGGTTACCTACTCCGTCCACCGGCAGCGCGGTCAGCATCTTGGTTACTGGCAAAAGGCAAATAAGGATCAaca                                                                                      <  2:630506/86‑1 (MQ=255)                   cGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAg                                                                              <  1:338612/100‑1 (MQ=255)                      cGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATa                                                                           <  2:1186764/100‑1 (MQ=255)                         gCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAgcg                                                                        <  1:315955/100‑1 (MQ=255)                            tAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCa                                                                     <  2:872846/100‑1 (MQ=255)                            tAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCa                                                                     <  1:2933085/100‑1 (MQ=255)                             aaTTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCaa                                                                    >  2:2421749/1‑100 (MQ=255)                              aTTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAg                                                                   <  1:749726/100‑1 (MQ=255)                              aTTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAg                                                                   <  1:3056490/100‑1 (MQ=255)                                   cccAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCaa                                                              >  2:2929790/1‑100 (MQ=255)                                   cccAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCaa                                                              <  1:2784784/100‑1 (MQ=255)                                       ggCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGc                                                          >  2:2535614/1‑100 (MQ=255)                                        gCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGcc                                                         <  2:1628602/100‑1 (MQ=255)                                           aCCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATc                                                      >  2:2386790/1‑100 (MQ=255)                                             cTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAg                                                    >  2:651334/1‑100 (MQ=255)                                              tAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAgg                                                   >  1:651431/1‑100 (MQ=255)                                              tAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAgg                                                   >  1:2305719/1‑100 (MQ=255)                                              tAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAgg                                                   >  2:42304/1‑100 (MQ=255)                                               aaTACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGc                                                  <  1:738539/100‑1 (MQ=255)                                               aaTACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGc                                                  >  1:1001486/1‑100 (MQ=255)                                               aaTACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGc                                                  >  1:1929706/1‑100 (MQ=255)                                                  aCATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGt                                               <  2:2337265/100‑1 (MQ=255)                                                  aCATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGt                                               <  1:1708820/100‑1 (MQ=255)                                                     tCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCTCCGGATAGCGCCAAGACCAACCGCCATCAGGCTGTTTa                                            >  2:1135238/1‑100 (MQ=255)                                                     tCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTa                                            <  2:1178959/100‑1 (MQ=255)                                                     tCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTa                                            <  2:2477955/100‑1 (MQ=255)                                                     tCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTa                                            <  1:190692/100‑1 (MQ=255)                                                      ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTAt                                           <  2:914136/100‑1 (MQ=255)                                                       cACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCGGGCTGTTTATc                                          <  1:1273212/100‑1 (MQ=255)                                                        aCCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCg                                         >  2:3026236/1‑100 (MQ=255)                                                        aCCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCg                                         <  1:601009/100‑1 (MQ=255)                                                         ccGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCg                                         <  1:37106/99‑1 (MQ=255)                                                           ggCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCa                                      >  2:268845/1‑100 (MQ=255)                                                             cAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAgg                                    >  1:1637899/1‑100 (MQ=255)                                                             cAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAgg                                    <  2:81317/100‑1 (MQ=255)                                                              aGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCGGTTTATCGCCAGGa                                   >  2:2077085/1‑100 (MQ=255)                                                               gcgcGGTCAGCAGCTGGTCTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGAc                                  >  1:617429/1‑100 (MQ=255)                                                                cgcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACg                                 >  1:544402/1‑100 (MQ=255)                                                                cgcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACg                                 >  1:1097898/1‑100 (MQ=255)                                                                 gcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCCGGACg                                 >  2:858981/1‑99 (MQ=255)                                                                    gTCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGTCCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGa                             <  2:1630080/100‑1 (MQ=255)                                                                     tCAGCAGCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAg                            <  2:200999/100‑1 (MQ=255)                                                                      cagcagCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAgg                           <  1:1807704/100‑1 (MQ=255)                                                                      cagcagCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAgg                           >  2:2929676/1‑100 (MQ=255)                                                                      cagcagCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAgg                           <  2:894433/100‑1 (MQ=255)                                                                      cagcagCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAgg                           >  1:1752772/1‑100 (MQ=255)                                                                      cagcagCTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAgg                           >  2:2353033/1‑100 (MQ=255)                                                                      cagcagCTGGTTTACTGGCAACAGACAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAgg                           <  2:1805954/100‑1 (MQ=255)                                                                       atcagcTCGTTTACTGTCAACAGACAAACAAGTCTCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCTGGATGCCGAgg                           <  2:1606351/97‑1 (MQ=255)                                                                        gcagcTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGat                         >  2:2033864/1‑100 (MQ=255)                                                                        gcagcTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGat                         >  1:1141752/1‑100 (MQ=255)                                                                          agcTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATTAGGCTGTTTATCGCCAGTACGCCGAGGattt                       <  1:2059990/100‑3 (MQ=255)                                                                          agcTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGatat                       <  1:2447725/100‑1 (MQ=255)                                                                            cTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCg                     >  2:2406812/1‑100 (MQ=255)                                                                            cTGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCg                     >  2:75938/1‑100 (MQ=255)                                                                             tGGTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCgg                    <  2:97731/100‑1 (MQ=255)                                                                              ggTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCgg                    <  1:2092851/99‑1 (MQ=255)                                                                              ggTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGt                   >  1:271825/1‑100 (MQ=255)                                                                              ggTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGt                   <  2:701344/100‑1 (MQ=255)                                                                              ggTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGt                   >  1:1910909/1‑100 (MQ=255)                                                                               gTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTa                  <  1:2137014/100‑1 (MQ=255)                                                                               gTTTACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTa                  >  1:2333429/1‑100 (MQ=255)                                                                                tttACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAg                 >  2:993180/1‑100 (MQ=255)                                                                                 ttACTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAgg                <  1:2684632/100‑1 (MQ=255)                                                                                    cTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGgaag             <  1:1385760/100‑1 (MQ=255)                                                                                    cTGGCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAACACCAAACGCCATCAGGCTGTTTATCGCCAGCACCCCGAGGATATCgc                    <  2:2899187/93‑2 (MQ=255)                                                                                      ggCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGgaagaa           >  2:1595796/1‑100 (MQ=255)                                                                                       gCAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGaagaaa          >  2:636978/1‑100 (MQ=255)                                                                                        cAACAGGGAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTAACGCCAGGACGCAGAGGATATCGGTAGGaagaaac         <  2:676960/100‑2 (MQ=255)                                                                                        cAACAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGaagaaag         >  2:2735185/1‑100 (MQ=255)                                                                                         aaCAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGaagaaaga        <  1:278/100‑1 (MQ=255)                                                                                          aCAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAagag       <  1:886068/100‑1 (MQ=255)                                                                                           cAGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAagag       >  2:2939107/1‑99 (MQ=255)                                                                                            aGGCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTa     >  2:61016/1‑100 (MQ=255)                                                                                             ggCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTaa    >  2:1610614/1‑100 (MQ=255)                                                                                             ggCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTaa    >  1:2413064/1‑100 (MQ=255)                                                                                             ggCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTaa    >  1:2324797/1‑100 (MQ=255)                                                                                             ggCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTaa    >  1:1032134/1‑100 (MQ=255)                                                                                             ggCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGACTaa    >  2:237997/1‑100 (MQ=255)                                                                                              gCAAATAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAAc   >  2:1994051/1‑100 (MQ=255)                                                                                               cAAATAAGGATCAACACGCCCCAGATCGCGCCAAGACCAAACGCAATCAGGCTGTTTATCGCCAGGACGCAGCGGATATCGGTAGGAAGAAAGAGTAAcc  <  2:901561/100‑1 (MQ=255)                                                                                                  |                                                                                                 TGCAAAAGCTGAAACCACCGGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACC  >  NC_000913/3506911‑3507104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑