Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,214,649	A→T	I124F (ATC→TTC)	metA →	homoserine O‑transsuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,214,649	0	A	T	100.0%	329.7 / NA	95	I124F (ATC→TTC)	metA	homoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (41/54);  total (41/54)

CTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTC  >  NC_000913/4214552‑4214745                                                                                                  |                                                                                                 cTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTc                                                                                                <  2:2014125/100‑1 (MQ=255)  tttGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCa                                                                                               >  2:2021874/1‑100 (MQ=255)    tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCaa                                                                                              <  2:2284124/99‑1 (MQ=255)    tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCa                                                                                               <  1:983086/98‑1 (MQ=255)      aaGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACa                                                                                           <  2:2754873/100‑1 (MQ=255)         atatTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGt                                                                                        >  2:1679806/1‑100 (MQ=255)         atatTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGt                                                                                        <  1:2334193/100‑1 (MQ=255)           gatctggATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGc                                                                                      <  1:579821/95‑1 (MQ=255)           atTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGc                                                                                      >  1:2740737/1‑100 (MQ=255)             tCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTg                                                                                    <  1:1988369/100‑1 (MQ=255)              cacgATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTgg                                                                                   <  2:1417941/97‑1 (MQ=255)               aGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTgg                                                                                   >  1:55543/1‑99 (MQ=255)                ngATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGcagatc                                                                                 >  2:579813/2‑95 (MQ=255)                ggATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAg                                                                                 <  2:428508/100‑1 (MQ=255)                ggATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAg                                                                                 <  1:2839420/100‑1 (MQ=255)                   tCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAg                                                                                 >  2:14301/1‑97 (MQ=255)                   tCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTgg                                                                              <  2:2720003/100‑1 (MQ=255)                   tCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTgg                                                                              <  2:1365822/100‑1 (MQ=255)                     aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                            <  2:2957843/100‑1 (MQ=255)                     aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                            >  2:393228/1‑100 (MQ=255)                     aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                            <  2:2711880/100‑1 (MQ=255)                     aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                            <  2:2265044/100‑1 (MQ=255)                      gAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCg                                                                           >  2:2504861/1‑100 (MQ=255)                        aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaa                                                                         >  1:1301724/1‑100 (MQ=255)                        aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaa                                                                         <  2:1071084/100‑1 (MQ=255)                          tttGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAg                                                                       <  1:2078107/100‑1 (MQ=255)                          tttGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAg                                                                       <  1:1122296/100‑1 (MQ=255)                            tGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGAt                                                                     >  2:2596559/1‑100 (MQ=255)                            tGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGAt                                                                     >  1:405484/1‑100 (MQ=255)                            tGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGAt                                                                     >  1:384215/1‑100 (MQ=255)                              aCGGTTTGATTGTAACTGGTGCGGCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCa                                                                   <  2:2338364/100‑1 (MQ=255)                               cGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCAc                                                                  <  1:2860190/100‑1 (MQ=255)                                 gTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGt                                                                >  1:2012745/1‑100 (MQ=255)                                    tGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCAc                                                             <  2:1579553/100‑1 (MQ=255)                                    tGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCAc                                                             <  1:289360/100‑1 (MQ=255)                                     gATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCAcc                                                            <  1:1303077/100‑1 (MQ=255)                                      aTTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAGTGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCt                                                           >  2:1634812/1‑100 (MQ=255)                                         gTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCatccct                                                         >  2:2986469/1‑94 (MQ=255)                                         gTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGa                                                        <  1:1376567/100‑1 (MQ=255)                                          tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCATACAGGTACTGGAGTGGTCGAAAGATCACGTCACCTCGAc                                                       >  2:2147870/1‑100 (MQ=255)                                          tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGAc                                                       >  2:1022967/1‑100 (MQ=255)                                           aaCTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACg                                                      <  2:2418345/100‑1 (MQ=255)                                             cTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCt                                                    >  1:2809915/1‑100 (MQ=255)                                               ggTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGt                                                  >  2:2730920/1‑100 (MQ=255)                                                gTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTCCTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGt                                                  >  1:2700286/1‑99 (MQ=255)                                                gTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGtt                                                 >  2:966542/1‑100 (MQ=255)                                                gTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGtt                                                 <  1:2263245/100‑1 (MQ=255)                                                gTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGtt                                                 >  2:2176028/1‑100 (MQ=255)                                                atctgccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGtt                                                 <  1:1489010/96‑1 (MQ=255)                                                 tGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGttt                                                <  1:2598571/100‑1 (MQ=255)                                                  gcgcCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTg                                               >  2:2085100/1‑100 (MQ=255)                                                  gcgcCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTg                                               <  2:256744/100‑1 (MQ=255)                                                  gcgcCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTg                                               <  2:495150/100‑1 (MQ=255)                                                    gccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGttagat                                             >  2:1488997/1‑96 (MQ=255)                                                      cgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                           <  2:405477/100‑1 (MQ=255)                                                      cgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                           <  2:384208/100‑1 (MQ=255)                                                       gcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctgc                                          <  1:191816/100‑1 (MQ=255)                                                        cTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctgct                                         <  2:1237415/100‑1 (MQ=255)                                                         tGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctgctg                                        >  1:1627010/1‑100 (MQ=255)                                                         tGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctgctg                                        >  1:1626830/1‑100 (MQ=255)                                                            gCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                     >  1:731276/1‑100 (MQ=255)                                                            gCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                     <  2:861704/100‑1 (MQ=255)                                                            gCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                     >  1:1490852/1‑100 (MQ=255)                                                            gCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                     <  1:1011434/100‑1 (MQ=255)                                                              cTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCgg                                   <  1:1135328/100‑1 (MQ=255)                                                              cTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCgg                                   <  1:1743712/100‑1 (MQ=255)                                                              cTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCgg                                   <  1:589916/100‑1 (MQ=255)                                                              cTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCgg                                   <  1:589978/100‑1 (MQ=255)                                                                   ggAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAg                              <  1:2042026/100‑1 (MQ=255)                                                                      gTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGcc                           >  1:899023/1‑100 (MQ=255)                                                                       tttAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcg                          >  1:729701/1‑100 (MQ=255)                                                                        ttAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgc                         <  1:1400155/100‑1 (MQ=255)                                                                         tAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgcg                        >  2:591738/1‑100 (MQ=255)                                                                          aaTGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                       <  2:1143929/100‑1 (MQ=255)                                                                           atgatgTCGCTTACTGGCCGCAGTTCAAACAGGTGCCGGAGTGGTCGCAAGATCACGTCACCCCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCt                      <  2:1822852/100‑1 (MQ=255)                                                                             gatgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCa                    <  1:2587613/100‑1 (MQ=255)                                                                              atgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCaa                   <  1:523290/100‑1 (MQ=255)                                                                              atgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCaa                   <  1:191982/100‑1 (MQ=255)                                                                                gTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAata                 <  1:2504895/100‑1 (MQ=255)                                                                                 tCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAatat                <  1:1572544/100‑1 (MQ=255)                                                                                  cGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATc               >  2:2893836/1‑100 (MQ=255)                                                                                   gCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATcc              >  1:149597/1‑100 (MQ=255)                                                                                    cTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATcc              <  1:1041351/99‑1 (MQ=255)                                                                                     ttACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCctc            <  1:1035367/100‑1 (MQ=255)                                                                                        cTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTAc         >  1:1073143/1‑100 (MQ=255)                                                                                         tGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACg        <  1:1400553/100‑1 (MQ=255)                                                                                          ggCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACgg       >  2:746494/1‑100 (MQ=255)                                                                                          ggCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACgg       >  1:325132/1‑100 (MQ=255)                                                                                           gCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGc      >  1:267460/1‑100 (MQ=255)                                                                                            ccGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCa     >  2:348319/1‑100 (MQ=255)                                                                                             cGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCAt    <  2:2923931/100‑1 (MQ=255)                                                                                             cGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCAt    >  1:2607220/1‑100 (MQ=255)                                                                                             cGCAGTTCAAACAGGTGCTGGAGTGGACGAAAGATCACGTCACCTCGACGCTGTTTATCTGCGGGGCGGTACAGGCCGCGCACAATATCCTCTACGGCAt    >  2:676946/1‑100 (MQ=255)                                                                                              gCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCAtt   <  2:1301712/100‑1 (MQ=255)                                                                                               cAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTc  >  1:1088423/1‑100 (MQ=255)                                                                                                  |                                                                                                 CTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTC  >  NC_000913/4214552‑4214745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑