Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,243,370 C→T L49L (CTG→CTA malG ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,243,3700CT96.9% 308.1 / ‑0.4 98L49L (CTG→CTAmalGmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (41/53);  total (44/54)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

AGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAG  >  NC_000913/4243272‑4243465
                                                                                                  |                                                                                               
agTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCa                                                                                                <  2:2162876/100‑1 (MQ=255)
 gTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAg                                                                                               <  1:2666868/100‑1 (MQ=255)
   aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGc                                                                                             <  2:2841484/100‑1 (MQ=255)
   aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGc                                                                                             <  2:877365/100‑1 (MQ=255)
    ccGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCt                                                                                            >  2:217260/1‑100 (MQ=255)
       ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGcc                                                                                         <  2:2909743/100‑1 (MQ=255)
       ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGcc                                                                                         >  2:247931/1‑100 (MQ=255)
        gAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCg                                                                                        <  1:1278969/100‑1 (MQ=255)
        gAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCg                                                                                        <  1:415318/100‑1 (MQ=255)
            tgCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCg                                                                                    <  2:2237378/99‑1 (MQ=255)
            ggCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCg                                                                                    >  1:2830301/1‑100 (MQ=255)
            ggCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCg                                                                                    <  1:167083/100‑1 (MQ=255)
             gCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCga                                                                                   >  1:2237407/1‑99 (MQ=255)
              cGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCa                                                                                  >  1:1329939/1‑100 (MQ=255)
              cGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCa                                                                                  >  1:1446927/1‑100 (MQ=255)
              cGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCa                                                                                  >  2:2491480/1‑100 (MQ=255)
                gTGGCGTAATGCGACCATCAGCCTGTTCAATGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCaaa                                                                                <  1:618367/100‑1 (MQ=255)
                 tGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAg                                                                               <  2:547245/100‑1 (MQ=255)
                  ggCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGt                                                                              >  2:332247/1‑100 (MQ=255)
                   gCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGCTTTGCTCCGGGATTAGGCTGCCGGTCGCaaatt                                                                              <  1:1523555/99‑3 (MQ=255)
                    cGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGttt                                                                            >  2:1426094/1‑100 (MQ=255)
                    cGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGttt                                                                            >  1:1012266/1‑100 (MQ=255)
                     gTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTc                                                                           >  1:2778996/1‑100 (MQ=255)
                      tAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTcc                                                                          >  2:1716984/1‑100 (MQ=255)
                      tAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATGTGCTCCGGGATTAGGCTGCCGGGCGCAAAGTGTcc                                                                          >  2:912470/1‑100 (MQ=255)
                       aaTGCTACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTccc                                                                         <  2:1147957/100‑1 (MQ=255)
                       aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTccc                                                                         <  1:870972/100‑1 (MQ=255)
                       aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTccc                                                                         >  1:465213/1‑100 (MQ=255)
                         tGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTg                                                                       <  2:1406978/100‑1 (MQ=255)
                           cGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGAc                                                                     <  1:168081/100‑1 (MQ=255)
                           cGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGAc                                                                     >  2:1979349/1‑100 (MQ=255)
                               cATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAg                                                                 >  1:2315534/1‑100 (MQ=255)
                                 tCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCg                                                               <  2:1202975/100‑1 (MQ=255)
                                 tCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCg                                                               >  2:2035608/1‑100 (MQ=255)
                                   aGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCAAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgag                                                             <  2:1908679/100‑1 (MQ=255)
                                    gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCTAAGTTTACCTGACGCAGCgcga                                                            >  1:1295007/1‑100 (MQ=255)
                                    gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga                                                            >  2:2793375/1‑100 (MQ=255)
                                    gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga                                                            >  1:1127899/1‑100 (MQ=255)
                                    gCCTGTTCAACGCCAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTACGGGATTAGGCTGCCGGTCGCAAAGTTTCGCTGACGCAGCgag                                                             >  2:1893394/1‑99 (MQ=255)
                                      cttttCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGATATTTGCTCCTCGATTAGGCTGCCGGTCGCAAAATTTCCCTCACGCAGCGAGAt                                                           <  2:2927874/96‑1 (MQ=255)
                                        gTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGc                                                        <  1:768299/100‑1 (MQ=255)
                                         ttCAACGCTAAAACCTAACGCCAGTTTCCGGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcg                                                       <  2:128726/100‑1 (MQ=255)
                                         ttCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcg                                                       <  1:1310108/100‑1 (MQ=255)
                                         ttCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcg                                                       <  1:2976605/100‑1 (MQ=255)
                                           cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac                                                     <  1:2137845/100‑1 (MQ=255)
                                           cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac                                                     <  2:2397016/100‑1 (MQ=255)
                                           cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac                                                     <  2:850312/100‑1 (MQ=255)
                                            aaCGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgacg                                                    <  2:1313854/100‑1 (MQ=255)
                                             aCGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgacga                                                   <  2:266076/100‑1 (MQ=255)
                                              cGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCgacgac                                                  >  1:2993354/1‑100 (MQ=255)
                                              cGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCgacgac                                                  <  2:63029/100‑1 (MQ=255)
                                               gCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGAcc                                                 <  1:1745586/100‑1 (MQ=255)
                                               gCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGAcc                                                 <  2:1679548/100‑1 (MQ=255)
                                               gCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGAcc                                                 <  1:325244/100‑1 (MQ=255)
                                                cTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCa                                                >  2:1813184/1‑100 (MQ=255)
                                                cTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCa                                                >  1:1221726/1‑100 (MQ=255)
                                                  aaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc                                              <  1:2936694/100‑1 (MQ=255)
                                                   aaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATca                                             >  2:969685/1‑100 (MQ=255)
                                                   aaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATca                                             <  2:235800/100‑1 (MQ=255)
                                                    aaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcagc                                           >  1:98218/1‑100 (MQ=255)
                                                     aCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcagc                                           <  1:1099808/100‑1 (MQ=255)
                                                      ccTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcagca                                          <  1:1836153/100‑1 (MQ=255)
                                                       cTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcagn                                           <  2:1808450/98‑2 (MQ=255)
                                                        tAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCagcagc                                        <  2:1812362/100‑1 (MQ=255)
                                                         aaCGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCg                                       <  1:1717004/100‑1 (MQ=255)
                                                         aaCGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCg                                       >  2:2718551/1‑100 (MQ=255)
                                                          aCGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCgg                                      >  2:1661906/1‑100 (MQ=255)
                                                          aCGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCgg                                      >  2:2367528/1‑100 (MQ=255)
                                                           cGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCggg                                     >  1:238933/1‑100 (MQ=255)
                                                            gCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGa                                    <  2:2545232/100‑1 (MQ=255)
                                                             ccAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGaa                                   >  1:2746406/1‑100 (MQ=255)
                                                               aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACa                                 <  1:16726/100‑1 (MQ=255)
                                                                  ttCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATaa                              >  1:2188940/1‑100 (MQ=255)
                                                                   tCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAAt                             <  1:2179631/100‑1 (MQ=255)
                                                                    ccAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACTACCATCAGCAGCGGGAACATAATc                            <  2:2870426/100‑1 (MQ=255)
                                                                    ccAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATc                            >  1:125062/1‑100 (MQ=255)
                                                                    ccAGTGATCCCAGAAGATTTGCTCCGGGATTAGGCTGCGGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAAt                             >  2:1890375/1‑99 (MQ=255)
                                                                      aGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATc                            <  2:888107/98‑1 (MQ=255)
                                                                      aGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc                          >  2:2379323/1‑100 (MQ=255)
                                                                         gATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGc                       >  1:1128514/1‑100 (MQ=255)
                                                                          aTCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg                      <  2:1445333/100‑1 (MQ=255)
                                                                            cccAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg                      <  1:2998977/98‑1 (MQ=255)
                                                                              cAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaa                  >  2:2998294/1‑100 (MQ=255)
                                                                                  agaTTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag              >  2:378540/1‑100 (MQ=255)
                                                                                    aTTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagta            >  1:81445/1‑100 (MQ=255)
                                                                                    aTTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagta            <  2:816336/100‑1 (MQ=255)
                                                                                     tttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag           <  1:1979372/100‑1 (MQ=255)
                                                                                     tttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTGTCCCGGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag           <  2:3037087/100‑1 (MQ=255)
                                                                                      ttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagc          <  1:2396124/100‑1 (MQ=255)
                                                                                      ttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagc          >  2:847846/1‑100 (MQ=255)
                                                                                      ttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagc          <  2:2830262/100‑1 (MQ=255)
                                                                                      ttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagc          <  1:784491/100‑1 (MQ=255)
                                                                                       tGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagca         >  1:2537290/1‑100 (MQ=255)
                                                                                       tGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagca         <  1:2998335/100‑1 (MQ=255)
                                                                                         cTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagt       >  2:335539/1‑100 (MQ=255)
                                                                                          tCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagta      >  1:1435995/1‑100 (MQ=255)
                                                                                           ccGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtag     <  1:2843240/100‑1 (MQ=255)
                                                                                              ggATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGtagcagtcgcag  >  1:1300886/1‑100 (MQ=255)
                                                                                                  |                                                                                               
AGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAG  >  NC_000913/4243272‑4243465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: