Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913811,7210TG38.3% 618.8 / 33.9 327V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (39/161);  new base G (120/4);  total (162/165)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

TGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGTTACCC  >  NC_000913/811484‑811962
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  
tgaatgccgggATACCGACCGTCTGCTGGGGGTGCTGCTGGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                                                                                                                                                                       <  2:4720086/242‑1 (MQ=255)
                    gTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCAAGCGGAGGGGACGGcccttgag                                                                                                                                                                                                                                       >  2:4301021/1‑225 (MQ=255)
                    gTCGGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATGGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                                                                                                                                                                       <  1:4301767/231‑1 (MQ=255)
                      ctggtgAAGGTTCGGCATGCAAACGGTTAAAAAACAAGCCATTGCAGCGTCATTTGGTCGGGCAGCCGCCCACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGGGGTTTGTGGACCGGGCTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTc                                                                                                                                                                                                                 <  2:1357753/251‑1 (MQ=255)
                                  ctgcATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGGGAAGGCCCTCGATCTCTCGCCGCCCAAGCTTGTTTAAGGACGCCCGa                                                                                                                                                                                                     >  1:3484942/1‑251 (MQ=255)
                                     cATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGCCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATCCCCCCACGTACTGGCCGCGGGTTGTGGCCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  1:5662769/250‑1 (MQ=255)
                                      aTGGAAACGTTAAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAGGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACGGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGa                                                                                                                                                                                                 <  2:5539364/251‑1 (MQ=255)
                                        ggCAACGTTTAAAAACCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGGGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:849349/247‑1 (MQ=255)
                                               gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc                                                                                                                                                                                                                                      <  2:2317828/205‑1 (MQ=255)
                                               gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCCTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGAAGGATGCCGCCGa                                                                                                                                                                                        >  1:1091117/1‑251 (MQ=255)
                                               gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCCTAGAtc                                                                                                                                                                                                                                      >  1:2318236/1‑205 (MQ=255)
                                               gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGGACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGGAGGTGACGGCCCTTGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGCAGGATGCCGccgg                                                                                                                                                                                        >  1:2907139/1‑248 (MQ=255)
                                                               ttGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGc                                                                                                                                                                                                                                              <  2:1665003/181‑1 (MQ=255)
                                                                       ncATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCCCGCGCAGGGGAAGGCCTTAGAtc                                                                                                                                                                                                                                      >  2:1602290/2‑181 (MQ=255)
                                                                       gCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc                                                                                                                                                                                                                                      <  1:1602591/181‑1 (MQ=255)
                                                                                 gggCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                                                                                                                                                                       <  1:4617584/170‑1 (MQ=255)
                                                                                    cAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACCCCACGCGCAGGGGACGGc                                                                                                                                                                                                                                              >  1:5865694/1‑160 (MQ=255)
                                                                                      gCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCCCGCGCAGGGGGCCGGCTTTGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGGAGGAGGCCGCAGACCCTTTTCTGGGGGGAGATACAGAAACCCTGcccgtaag                                                                                                                                                >  2:3387906/1‑245 (MQ=255)
                                                                                      gCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                                                                                                                                                                       <  2:5629942/165‑1 (MQ=255)
                                                                                      gCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCCCGCGCAGGGGACGGCCTTAGaa                                                                                                                                                                                                                                       >  1:5630878/1‑164 (MQ=255)
                                                                                      gCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCCCCGGTGACGGCCTTAGATCTCTCGCCGCCCAAGGTTGTTCAGGCACGCCAGGAGGGTGCCGCCGAGCATTATCt                                                                                                                                                                               >  1:1015038/1‑221 (MQ=255)
                                                                                            cacTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCACGCGCAGGGGACGGCCTTTGAt                                                                                                                                                                                                                                       >  1:3322260/1‑159 (MQ=255)
                                                                                            cacTAGGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGc                                                                                                                                                                                           <  2:5847253/203‑1 (MQ=255)
                                                                                              cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGGGACGGCCCTAGATCTCCCGCCGCCAATGCCTGTTCCGGCACGCCAGAAGGATGCCCCAGACCCTTATCTGGCGGGGGATAACTAAACCCCGcccgttgcggcctcga                                                                                                                                        >  1:817329/1‑237 (MQ=255)
                                                                                              cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                                                                                                                                                                       <  1:4094452/157‑1 (MQ=255)
                                                                                              cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAGGATGCCGCCGACCCTTATCTGGCGGGGGATATCGAAATCCTGCCCGTAGCGACTGCGa                                                                                                                                        >  2:3336504/1‑251 (MQ=255)
                                                                                              cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCCCGCGCAGGGggcggc                                                                                                                                                                                                                                              >  1:4773222/1‑150 (MQ=255)
                                                                                              cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTAGCGCCTTACTGGCAATGCTTCCACAGCGTAAATCCCCCCACGTCCTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtctct                                                                                                                                                                                                                                   <  1:182592/161‑1 (MQ=255)
                                                                                                       aCATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGc                                                                                                                                                                                                                                              <  2:2853414/141‑1 (MQ=255)
                                                                                                         aTGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCCGGGGACGGCCCCAGATCTCTCGCCGCCCAAGCTTGTTCAGGCACCCCCGAAGGATGCCGCCGACCCTTATCTTGCGGGGGATATCGAATc                                                                                                                                                            >  1:1700133/1‑220 (MQ=255)
                                                                                                            cAGATCTACAGCGCCAGGGTGCTGACGCCTTACGGGCAATGCTTCCACAGCGTAAATCCCCCCGCGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:3077517/179‑1 (MQ=255)
                                                                                                            cAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCACGCGCAGGGGACGGCCTTAGATCTCCCGCCCCCAATGCTTGTTCAGGCACGcccaaag                                                                                                                                                                                                   >  1:3078016/1‑174 (MQ=255)
                                                                                                              gATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                                                                                                                                                                       <  1:3098730/141‑1 (MQ=255)
                                                                                                              gATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCt                                                                                                                                                                                                                      <  2:5685912/158‑1 (MQ=255)
                                                                                                              gATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACCCGCAGGGGACGGGCTTAGATCTCTCGCCCCCCATGCTTTTTCAGGCACGCCCGAAGGATGCCCCAGGCCCCTTTCTGGGGGGGGATATCGCATCCccgccg                                                                                                                                                     >  2:1547965/1‑222 (MQ=255)
                                                                                                              gATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGAAg                                                                                                                                                                                                   >  1:3847855/1‑177 (MQ=255)
                                                                                                                 cTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCACGCGGCGGGGGCGGGCTTAGAt                                                                                                                                                                                                                                       >  2:5393810/1‑138 (MQ=255)
                                                                                                                         ccAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc                                                                                                                                                                                                                                      <  2:4683726/131‑1 (MQ=255)
                                                                                                                         ccAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGACGGCCTTAGAt                                                                                                                                                                                                                                       >  1:2483079/1‑130 (MQ=255)
                                                                                                                         ccAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCACGCGCAGGGGAGGGCCCTAGAtc                                                                                                                                                                                                                                      >  1:4684528/1‑131 (MQ=255)
                                                                                                                                       ccTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGCCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   >  2:4785806/1‑152 (MQ=255)
                                                                                                                                           aCTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGa                                                                                                                                                                                                 <  1:2618950/150‑1 (MQ=255)
                                                                                                                                           aCTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCAGAAGGa                                                                                                                                                                                                 >  2:2618512/1‑150 (MQ=255)
                                                                                                                                              ggCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc                                                                                                                                                                                                                                      <  2:4442204/110‑1 (MQ=255)
                                                                                                                                              ggCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCCCAGGGGACGGCCTTAGAtc                                                                                                                                                                                                                                      >  1:4442972/1‑110 (MQ=255)
                                                                                                                                                   tGCTTCCACAGCGTAAATCCCCCCACGTCCTGGAGGCGGGTTGTGGCCCTGGCTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATcc                                                                                                                                                           <  2:3477187/179‑1 (MQ=255)
                                                                                                                                                   tGCTTCCACAGCGTAAATCCCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGc                                                                                                                                                                                           <  1:1520414/148‑1 (MQ=255)
                                                                                                                                                   tGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc                                                                                                                                                                                                                                      <  1:1331813/105‑1 (MQ=255)
                                                                                                                                                   tGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACCCGCCGGTGACCGCCCTAGATCCCTCGCCGCCAATGCt                                                                                                                                                                                                                      >  2:3290622/1‑121 (MQ=255)
                                                                                                                                                             gcatAAATCCCCCCACGAACGGCGCGGGGGTTGGGGCCGGGGGGGGAGGGGCCGCCACGGGCGGGAACGTCACGCGCAGGTGACGGCTTTAGACCTCTCGCCGCCAATGCTTGTTAAGGCACGCCAGAAGGAGGCCGCGGACCATTATCTGGGGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTGCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg                                                                                             <  1:3181005/228‑1 (MQ=255)
                                                                                                                                                             gCGTAAATCCCCCCACGTCCTGGCCGGGGGTTGGGGCCTGGGGTGGAGGGGCCGCCACGGGCGGGAACGTCCCGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAgtggtg                                                                                                <  1:2147157/228‑1 (MQ=255)
                                                                                                                                                             gCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:5304384/130‑1 (MQ=255)
                                                                                                                                                             gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:1835924/130‑1 (MQ=255)
                                                                                                                                                             gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTTGATCTCTCGCCCCCAATGCTTGGTCCGGCACGCCAGAAg                                                                                                                                                                                                   >  1:1836261/1‑130 (MQ=255)
                                                                                                                                                             gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCCGGGGAAGGCCTTAGATCCTTCCCCGCCCAAGCTTTGTCAAGGACGCCCGAAGGAATCCGCCGACCATTATTTTGCGGGGGGTATTGGATTCCTTGCGTTTACCGCCTCGGCCGTTGGTCTTGCCTTGGGGAAACTCGCCGGGCAGTGGTgggggtgtttctttttcaacat                                                                           >  2:3180501/1‑231 (MQ=255)
                                                                                                                                                             gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACCTCCCGCGCAGGGGACGGGCTTAGATTTCTCGCCGCCAATGCTTGTTCAAGCACGCCCGaaa                                                                                                                                                                                                   >  2:5098468/1‑129 (MQ=255)
                                                                                                                                                             gCGTAAATACACCCACGTACGGGACGCGGGTTGGGGACCTGGCGGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATcc                                                                                                                                                           <  2:3002358/169‑1 (MQ=255)
                                                                                                                                                               gTAAATACCCCCACGTACTGGACGCGGGTTGTGGCCCGGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:3774544/128‑1 (MQ=255)
                                                                                                                                                                      cccccACGTACTGGACGCGGTTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCGGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:1793550/119‑1 (MQ=255)
                                                                                                                                                                        cccACGTACGGGACGCGGGTTGTGGACTTGGCGGGAGGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGAcc                                                                                                                                                                                      <  1:3023629/132‑1 (MQ=255)
                                                                                                                                                                          ccggtccTGGCCGCGGTTTGGGGCCCTGGCTGGATGACCCGCCCCTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGc                                                                                                                    <  1:5307892/189‑1 (MQ=255)
                                                                                                                                                                          cccGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCgccgcc                                                                                                                                                                                                                            <  1:2747688/90‑1 (MQ=255)
                                                                                                                                                                          cACGTGCTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCt                                                                                                                                                                                                                      <  2:3686373/98‑1 (MQ=255)
                                                                                                                                                                          cACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCACGCGCAGGGGACGGCCTTCGATCTCTCGCCGCCCAAGCTTTTTCCGGCACGCCAGAAGGa                                                                                                                                                                                                 >  1:505735/1‑119 (MQ=255)
                                                                                                                                                                          cACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGAAGTCACGCGGAGGTGGCggg                                                                                                                                                                                                                                              >  1:5430563/1‑73 (MQ=255)
                                                                                                                                                                          cACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTc                                                                                                                                                                                                                 <  2:448686/103‑1 (MQ=255)
                                                                                                                                                                          cACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  1:3671463/117‑1 (MQ=255)
                                                                                                                                                                          cACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGACGGGCTTAGATCTCTCGCCGCCAATGCTTGGTCAGGGACGCCAGAAg                                                                                                                                                                                                   >  1:995077/1‑117 (MQ=255)
                                                                                                                                                                            cGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGGGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTTGATCTTGCATGGAGCAATCTCTCAGTGCAGGGGGGCgg                                                                                             >  2:2079280/1‑216 (MQ=255)
                                                                                                                                                                            cGTACGGGACGCGGGTTGTGGCCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGCCGGCTTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg                                                                                             <  1:2079654/216‑1 (MQ=255)
                                                                                                                                                                             gtccTGGGCGCGGGTTGTGGCCCTGGTTGGTTGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCt                                                                                                                                                                               <  1:2815465/131‑1 (MQ=255)
                                                                                                                                                                             gTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCACGCGGAGGGGAAGGGCTTTGGTCTCTCGCCGCCAAAGGTTGTTTAGGGACGGCAGAAGGATGGCGCAGAACATTAttt                                                                                                                                                                               >  2:2815010/1‑132 (MQ=255)
                                                                                                                                                                              tgcgggccggggtttggggcctgggcgggATGGGCCCCCACTGGCGGGACCGTCACGCGCAGGTGACGGCCTTAGACCTCTCGCCGCCAGTGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGggt                                                         <  1:1970096/227‑1 (MQ=255)
                                                                                                                                                                                   gACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGGCGGCCTTAGATCTCTCGCCGCCAATTCTTGTTCAGGCACGCCAGAAGGATGCCGCCGACCCTTATCTgg                                                                                                                                                                             >  2:1125486/1‑130 (MQ=255)
                                                                                                                                                                                         ggTTTTGAACCTGCCGGGGGGAGCCGCCACTGGCGGAACGGTCCCGCGCAGGTGGCGGCTTTAGACCTCTCGCCCCCAATGCTTGTTCAGCCCCGCCAGAGGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAAtct                                                                                                               <  1:3702462/185‑1 (MQ=255)
                                                                                                                                                                                         ggTTGTGGCCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCg                                                                                                                                                                <  1:4992004/136‑1 (MQ=255)
                                                                                                                                                                                          gtgggggcccTGGCGGGAGGGCCCGCCACGGGCGGGAACGCCCCGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAgtgg                                                                                                  <  2:2177613/189‑1 (MQ=255)
                                                                                                                                                                                          gTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCCGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCCCGCCAGAAg                                                                                                                                                                                                   >  2:719205/1‑101 (MQ=255)
                                                                                                                                                                                          gTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGGGATATCGAAACCCTGCCGGTAGCGACTGCGGCGTTCGATCTTGCATGGGGCAAACTCGCCGTGCCGTGGGGCGGGAAttttttc                                                                                   >  2:2375243/1‑208 (MQ=255)
                                                                                                                                                                                          gTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  1:719349/101‑1 (MQ=255)
                                                                                                                                                                                             gtgGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGGACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGggga                                                                                                                                                                      >  1:4565262/1‑127 (MQ=255)
                                                                                                                                                                                                    tggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAAAGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCCTTATCTGGCGGGAGATATCGAATCCCTGCCg                                                                                                                                                     >  1:1418164/1‑136 (MQ=255)
                                                                                                                                                                                                    gggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCg                                                                                                                                                     <  2:1417892/135‑1 (MQ=255)
                                                                                                                                                                                                     ggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACCCCCGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATcc                                                                                                                                                           >  1:114481/1‑129 (MQ=255)
                                                                                                                                                                                                     ggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATcc                                                                                                                                                           <  2:114432/129‑1 (MQ=255)
                                                                                                                                                                                                     ggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCCCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCCGACCATTATCTGGCGGGGGATATCGAATCCCCGCCGTTAGCGACTGCGCCGTTCGATCTTGCATGGAGCAATCTCGCACTGCAGTGGGGCGGGAATTTATCCCCGGCACTCCGCGAGCTGTGTCGGGGGGGGGGGCCCCAAgaggggggcgg                                 >  2:4126316/1‑241 (MQ=255)
                                                                                                                                                                                                     ggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTTAGGCACGCCAGAAGGATGCCGCAGACCCTTATCTGGCGGGGGATATCCAATTCCTGCCGTTAGCGACCGCGGCGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAgtgg                                                                                                  >  1:4876623/1‑186 (MQ=255)
                                                                                                                                                                                                     ggctggATGAGCCGCCACTGGCGGGAAAGTTACGCGCCGGGGACGGCCCTAGATCTCCCCCCGCCCAAGCTTGTTCAGGCACGCCCGAAGGATGCCGCAGACCCTTATCTGGCGGGGGATATTGTATCCCTGCCGTTAGCGACTGCGCCGGTCGATCTTGCATGGAGGAAACCCGCAGGGCAgtgg                                                                                                  >  2:1973191/1‑186 (MQ=255)
                                                                                                                                                                                                         ggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAAAGCTTGGTCAGGCCCGCCAGAAGGGTGCCGCAGACCATTATCt                                                                                                                                                                               >  1:3412120/1‑106 (MQ=255)
                                                                                                                                                                                                              gcccgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGAc                                                                                                                                             <  1:3350613/132‑1 (MQ=255)
                                                                                                                                                                                                                        ggCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAgtgg                                                                                                  >  1:5699100/1‑167 (MQ=255)
                                                                                                                                                                                                                            ggAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                                                                                                                                                                   <  2:4594542/67‑1 (MQ=255)
                                                                                                                                                                                                                             gAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCg                                        >  2:4071264/1‑220 (MQ=255)
                                                                                                                                                                                                                                 gTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGggtgg                                                       >  1:5197786/1‑201 (MQ=255)
                                                                                                                                                                                                                                 gTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGggtgg                                                       <  2:5196903/201‑1 (MQ=255)
                                                                                                                                                                                                                                    ccgcgCAGGTGCCGCCCTTAGCCCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGTTAccc  <  2:5003971/250‑1 (MQ=255)
                                                                                                                                                                                                                                         cAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCg                                                                                                                                                                >  2:3107918/1‑88 (MQ=255)
                                                                                                                                                                                                                                           ggTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGGGCGCCCCAAAGGCGTGGTGGCGTTTACAACGCTGGTGCAGGGa           >  1:5388066/1‑235 (MQ=255)
                                                                                                                                                                                                                                           ggTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACCCCAGAAGGATGCCGCAGACCATTATCTGGGGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCg                                                            <  1:1129002/186‑1 (MQ=255)
                                                                                                                                                                                                                                           ggTGACGGCCTTAGATCTCCCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGGGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCt                                                                                                                              <  2:3546224/120‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  
TGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGTTACCC  >  NC_000913/811484‑811962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: