Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,915,743	0	A→G	100.0%	0.6	1	V77A (GTG→GCG)	atpC	F1 sector of membrane‑bound ATP synthase, epsilon subunit
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/0):  ref base (0/0):  total (1/0)

TTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGATCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGC  >  NC_000913/3915540‑3915749                                                                                                                                                                                                            |       tttCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTTAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGATCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCGCGTTGc  >  2:212219/1‑210 (MQ=255)                                                                                                                                                                                                            |       TTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGATCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGC  >  NC_000913/3915540‑3915749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.